MG1655
W3110
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Gene Report : mngA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  mngA
Related strains
     Strains Search Search strains by gene name:  mngA   Search strains by all related name:  mngA b0731 ECK0719 hrsA JW0720

General information  (Go to Linear View:)
 Gene Name mngA  
 Alternative name b0731,ECK0719,hrsA,JW0720  
 Location, Length 766,406 - 768,382 (  +  ) ;   16.49 min ; 1977 (bp) ,   658 (aa) Go to Linear View
 Product fused 2-O-a-mannosyl-D-glycerate specific PTS enzyme IIABC components  
 Note ECK0719:JW0720:b0731  
 Function  
 PID 85674750  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) HRSA_ECOLI ( P54745 )
    -  Protein name RecName: Full=Heat-responsive suppressor hrsA; AltName: Full=Putative PTS system EIIABC component; Includes: RecName: Full=Phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=PTS system EIIA component; Includes: RecName: Full=Phosphotransferase enzyme IIB component; EC=2.7.1.69; AltName: Full=PTS system EIIB component; Includes: RecName: Full=Permease IIC component; AltName: Full=PTS system EIIC component;  
    -  Synonyms  
    -  Gene name Name=hrsA; OrderedLocusNames=b0731, JW0720;  
    -  Comments
  • FUNCTION:Involved in thermoinduction of ompC.
  • FUNCTION:The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (By similarity).
  • CATALYTIC ACTIVITY:Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
  • CATALYTIC ACTIVITY:Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
  • SUBCELLULAR LOCATION:Cell inner membrane; Multi-pass membrane protein.
  • DOMAIN:The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
  • DOMAIN:The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
  • DOMAIN:The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
  • SIMILARITY:Contains 1 PTS EIIA type-2 domain.
  • SIMILARITY:Contains 1 PTS EIIB type-2 domain.
  • SIMILARITY:Contains 1 PTS EIIC type-2 domain.  

    •  Linear View
    View Location
       755.0  –  780.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP  
        PDB
             PSI-BLAST  
        SWISS-PROT
             BLAST RecName: Full=Heat-responsive suppressor hrsA; AltName: Full=Putative PTS system EIIABC component; Includes: RecName: Full=Phosphotransferase enzyme IIA component; AltName: Full=PTS system EIIA component; Includes: RecName: Full=Phosphotransferase enzyme IIB component; AltName: Full=PTS system EIIB component; Includes: RecName: Full=Permease IIC component; AltName: Full=PTS system EIIC component  
            PSI-BLAST
        nr
             BLAST fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component [Escherichia coli str. K-12 substr. MG1655]  
    Pfam 27.0   (database updated : 2013-03 )
        Pfam

    Other Cross-Reference
        EcoCyc mngA 

    MMBR References
    Biosci Biotechnol Biochem. 1996;60(2):309-15.
    Isolation and characterization of the heat-responsive genes in Escherichia coli.
    Utsumi R, Horie T, Katoh A, Kaino Y, Tanabe H, Noda M. ( 9063979 )

    Sequences
    Amino acid
    FASTA format 
    0001 MVLFYRAHWR DYKNDQVRIM MNLTTLTHRD ALCLNARFTS REEAIHALTQ RLAALGKISS TEQFLEEVYR 
0071 RESLGPTALG EGLAVPHGKT AAVKEAAFAV ATLSEPLQWE GVDGPEAVDL VVLLAIPPNE AGTTHMQLLT 
0141 ALTTRLADDE IRARIQSATT PDELLSALDD KGGTQPSASF SNAPTIVCVT ACPAGIAHTY MAAEYLEKAG 
0211 RKLGVNVYVE KQGANGIEGR LTADQLNSAT ACIFAAEVAI KESERFNGIP ALSVPVAEPI RHAEALIQQA 
0281 LTLKRSDETR TVQQDTQPVK SVKTELKQAL LSGISFAVPL IVAGGTVLAV AVLLSQIFGL QDLFNEENSW 
0351 LWMYRKLGGG LLGILMVPVL AAYTAYSLAD KPALAPGFAA GLAANMIGSG FLGAVVGGLI AGYLMRWVKN 
0421 HLRLSSKFNG FLTFYLYPVL GTLGAGSLML FVVGEPVAWI NNSLTAWLNG LSGSNALLLG AILGFMCSFD 
0491 LGGPVNKAAY AFCLGAMANG VYGPYAIFAS VKMVSAFTVT ASTMLAPRLF KEFEIETGKS TWLLGLAGIT 
0561 EGAIPMAIED PLRVIGSFVL GSMVTGAIVG AMNIGLSTPG AGIFSLFLLH DNGAGGVMAA IGWFGAALVG 
0631 AAISTAILLM WRRHAVKHGN YLTDGVMP
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             TCTCATTAAT ACGAATTTAA CCATTATGCC 
-070 CGATAAATTC ATCCTGTAAA TAATACAAAT ACAATACAAA TAATTTCAAT CAAGTGAAAT TGATCACATA 
0001 atggtattgt tttatcgggc acactggcgc gactataaaa acgatcaagt gaggatcatg atgaatctga 
0071 cgactctgac ccaccgcgat gcgttgtgtc tgaatgcgcg ctttaccagc cgtgaagagg ccatccacgc 
0141 gttgactcaa cgtcttgctg ctctggggaa aatttccagt actgagcaat ttctggaaga agtgtatcgc 
0211 cgtgaaagcc ttggcccgac cgccttaggt gaagggttgg ctgtgccgca tggcaaaact gctgcggtaa 
0281 aagaagcggc gtttgcggtc gccacactca gcgagccgct tcagtgggaa ggcgttgatg gcccggaagc 
0351 agttgattta gtggtgctgc tggcgattcc ccccaatgaa gcgggtacta cgcatatgca actgctgaca 
0421 gcgctgacca cgcgccttgc ggatgatgag attcgggcgc gtatacagtc ggcgacgacg cctgatgagt 
0491 tgctctcggc gctggatgac aagggaggca cgcaaccttc tgcctctttt tccaacgcgc caactatcgt 
0561 ctgcgtaacg gcctgtccgg cgggtattgc tcacacctat atggctgcgg aatatctgga aaaagccgga 
0631 cgcaaactcg gcgtaaatgt ttacgttgaa aaacaaggcg ctaacggcat tgaagggcgt ttaacggcgg 
0701 atcaactcaa tagtgcaacc gcctgtattt ttgcggctga agtcgccatc aaggagagtg agcgttttaa 
0771 tggcattccc gcgctttcag tgcctgttgc cgagccgatt cgccatgcag aagcgttgat ccaacaagcg 
0841 cttaccctca agcgtagcga tgagacgcgt accgtacagc aagatacgca accggtgaaa agtgtcaaaa 
0911 cggagctgaa acaggcactg ttgagcggaa tctcttttgc cgtaccgttg attgtcgcgg ggggcacggt 
0981 gctggcggtc gcggtattac tgtcgcaaat cttcgggcta caagatctgt ttaatgaaga aaactcctgg 
1051 ctgtggatgt accgcaagct gggcggcggg ctgctcggaa ttttgatggt accggtgctc gcggcctata 
1121 ccgcctattc tctggcagat aaaccggcgt tagcgccagg ctttgcggct ggacttgccg ccaacatgat 
1191 cggctccggg tttctcggcg cggtcgttgg cggattgata gccggttact tgatgcgctg ggtgaaaaat 
1261 cacttgcgtc ttagcagtaa attcaatgga ttcctgactt tttatctcta cccggtgctc ggtacgttgg 
1331 gagcgggcag tctgatgctg tttgtggtgg gggaacctgt cgcctggatc aataactcgc ttaccgcctg 
1401 gctgaacggt ctgtcaggaa gtaacgcgct gttgctgggt gccattctcg gttttatgtg ttcctttgac 
1471 cttggagggc cagtgaataa agccgcttat gcattctgcc tgggcgcaat ggcgaacggc gtttacggcc 
1541 cgtatgccat tttcgcctcc gtcaaaatgg tttcggcatt taccgtaacc gcttccacga tgctcgcacc 
1611 gcgcctgttt aaagagtttg aaattgagac cgggaaatcc acctggctgt tagggctggc aggtattacc 
1681 gaaggggcga tcccgatggc gattgaagat ccgctgcggg ttattggttc gtttgtgctg ggctctatgg 
1751 taacgggcgc tattgtcggt gcgatgaata tcggcctttc gacacccggt gccggcattt tctcgctctt 
1821 tttacttcat gataatggcg cgggcggtgt tatggcggca attggctggt ttggcgcggc attggtgggg 
1891 gctgcaatct cgactgcaat tctcctgatg tggcggcgtc acgcggttaa gcatggcaac tatctgactg 
1961 atggcgtaat gccataaACA AAAAGGAAAC GACGATGAAA GCAGTATCTC GCGTTCACAT CACCCCGCAT 
2031 ATGCACTGGG ATCGAGAGTG GTATTTCACC ACCGAAGAGT CACGTAT