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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Related gene (MG1655 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
lipA
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Related strains
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Strains Search
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Search strains by gene name:
lipA
Search strains by all related name:
lipA b0628 ECK0621 JW0623 lip
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General information (Go to Linear View: )
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Gene Name
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lipA
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Alternative name
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b0628,ECK0621,JW0623,lip
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Location, Length
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659,673 - 660,638
(
-
)
;
14.20
min
;
966
(bp) ,
321
(aa)
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Product
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lipoate synthase
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Note
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ECK0621:JW0623:b0628
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Function
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PID
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1651257
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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LIPA_ECOLI
(
P60716
;
P25845; P77595
)
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- Protein name
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RecName: Full=Lipoyl synthase; EC=2.8.1.8; AltName: Full=Lipoic acid synthase; AltName: Full=Lipoate synthase; AltName: Full=Sulfur insertion protein lipA; AltName: Full=Lip-syn; Short=LS;
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- Synonyms
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- Gene name
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Name=lipA; Synonyms=lip; OrderedLocusNames=b0628, JW0623;
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- Comments
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FUNCTION:Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. Free octanoate is not a substrate for lipA.
CATALYTIC ACTIVITY:Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine.
COFACTOR:Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
ENZYME REGULATION:Inhibited by the AdoMet analog S-adenosyl homocysteine.
PATHWAY:Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]:step 2/2.
SUBUNIT:Monomer or homodimer.
SUBCELLULAR LOCATION:Cytoplasm.
MISCELLANEOUS:The source of sulfurs is the lipA protein itself, most likely one 4Fe-4S cluster.
SIMILARITY:Belongs to the radical SAM superfamily. Lipoyl synthase family.
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MMBR References
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J Bacteriol. 1991;173(20):6411-20.
Lipoic acid metabolism in Escherichia coli: isolation of null mutants defective in lipoic acid biosynthesis, molecular cloning and characterization of the E. coli lip locus, and identification of the lipoylated protein of the glycine cleavage system.
Vanden Boom TJ, Reed KE, Cronan JE Jr.
(
1655709
)
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| Sequences |
Amino acid
FASTA format
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0001 MSKPIVMERG VKYRDADKMA LIPVKNVATE REALLRKPEW MKIKLPADST RIQGIKAAMR KNGLHSVCEE
0071 ASCPNLAECF NHGTATFMIL GAICTRRCPF CDVAHGRPVA PDANEPVKLA QTIADMALRY VVITSVDRDD
0141 LRDGGAQHFA DCITAIREKS PQIKIETLVP DFRGRMDRAL DILTATPPDV FNHNLENVPR IYRQVRPGAD
0211 YNWSLKLLER FKEAHPEIPT KSGLMVGLGE TNEEIIEVMR DLRRHGVTML TLGQYLQPSR HHLPVQRYVS
0281 PDEFDEMKAE ALAMGFTHAA CGPFVRSSYH ADLQAKGMEV K
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 gatatactgc cagtcgttaa ttcaaaaata
-070 gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc gcaactggaa cacgcacgct
0001 atgagtaaac ccattgtgat ggaacgcggt gttaaatacc gcgatgccga taagatggcc cttatcccgg
0071 ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca agcttccagc
0141 ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa
0211 gcctcctgcc ctaacctggc ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt
0281 gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca atgaaccagt
0351 gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca cctccgttga ccgtgatgac
0421 ctgcgcgatg gcggtgccca gcactttgcg gattgcatta ctgccattcg ggaaaaaagc ccgcaaatca
0491 aaattgaaac tctggtgccg gatttccgcg gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc
0561 accagatgtg ttcaaccata acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc tggtgcagat
0631 tacaactggt cgctgaagct gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc
0701 tgatggtggg actgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt
0771 gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc cggttcaacg ttacgttagc
0841 ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga tgggctttac ccatgctgca tgcggtccgt
0911 ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg gatggaagtt aagtaatcct gtaataatta
0981 cttacatctg tctgataaaa aacccgcttt ttgaagcggg ttttttgtat cgaacaagat attgagggag
1051 cgtcctgctc gccacg
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