MG1655
W3110
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Gene Report : pdxA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  pdxA
Related strains
     Strains Search Search strains by gene name:  pdxA   Search strains by all related name:  pdxA b0052 ECK0053 JW0051

General information  (Go to Linear View:)
 Gene Name pdxA  
 Alternative name b0052,ECK0053,JW0051  
 Location, Length 52,427 - 53,416 (  -  ) ;   1.13 min ; 990 (bp) ,   329 (aa) Go to Linear View
 Product 4-hydroxy-L-threonine phosphate dehydrogenase, NAD-dependent  
 Note ECK0053:JW0051:b0052  
 Function  
 PID 21321932  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) PDXA_ECOLI ( P19624 )
    -  Protein name RecName: Full=4-hydroxythreonine-4-phosphate dehydrogenase; EC=1.1.1.262; AltName: Full=4-(phosphohydroxy)-L-threonine dehydrogenase;  
    -  Synonyms  
    -  Gene name Name=pdxA; OrderedLocusNames=b0052, JW0051;  
    -  Comments
  • FUNCTION:Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).
  • CATALYTIC ACTIVITY:4-(phosphonooxy)-L-threonine + NAD(+) = (2S)-2-amino-3-oxo-4-phosphonooxybutanoate + NADH.
  • COFACTOR:Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt.
  • BIOPHYSICOCHEMICAL PROPERTIES:Kinetic parameters:KM=85 uM for 4-(phosphohydroxy)-L-threonine (at 0.1 M Tris-HCl and pH 7.5); KM=113 uM for 4-(phosphohydroxy)-L-threonine (at 0.1 M phosphate buffer and pH 7.5); Vmax=2.8 umol/min/mg enzyme with 4-(phosphohydroxy)-L-threonine as substrate (at 0.1 M Tris-HCl and pH 7.5); Vmax=0.66 umol/min/mg enzyme with 4-(phosphohydroxy)-L-threonine as substrate (at 0.1 M phosphate buffer and pH 7.5);
  • PATHWAY:Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate:step 4/5.
  • SUBUNIT:Homodimer.
  • SUBCELLULAR LOCATION:Cytoplasm (By similarity).
  • MISCELLANEOUS:The active site is located at the dimer interface.
  • MISCELLANEOUS:According to PubMed:15026039, pdxA may catalyze both oxidation and decarboxylation reactions that directly lead to AHAP.
  • SIMILARITY:Belongs to the pdxA family.  

    •  Linear View
    View Location
       40.0  –  65.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP  
        PDB
             PSI-BLAST  
        SWISS-PROT
             BLAST RecName: Full=4-hydroxythreonine-4-phosphate dehydrogenase; AltName: Full=4-  
             PSI-BLAST RecName: Full=4-hydroxythreonine-4-phosphate dehydrogenase; AltName: Full=4-
        nr
             BLAST 4-hydroxy-L-threonine phosphate dehydrogenase, NAD-dependent [Escherichia coli str. K-12 substr. MG1655]  
    Pfam 27.0   (database updated : 2013-03 )
        Pfam

    Other Cross-Reference
        EcoCyc pdxA 

    Sequences
    Amino acid
    FASTA format 
    0001 MVKTQRVVIT PGEPAGIGPD LVVQLAQREW PVELVVCADA TLLTNRAAML GLPLTLRPYS PNSPAQPQTA 
0071 GTLTLLPVAL RAPVTAGQLA VENGHYVVET LARACDGCLN GEFAALITGP VHKGVINDAG IPFTGHTEFF 
0141 EERSQAKKVV MMLATEELRV ALATTHLPLR DIADAITPAL LHEVIAILHH DLRTKFGIAE PRILVCGLNP 
0211 HAGEGGHMGT EEIDTIIPVL NELRAQGMKL NGPLPADTLF QPKYLDNADA VLAMYHDQGL PVLKYQGFGR 
0281 GVNITLGLPF IRTSVDHGTA LELAGRGKAD VGSFITALNL AIKMIVNTQ
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             gtgcataccg catgctgatg aaccgtaagt 
-070 tctcggaaga agcagcaagc tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc tgagcaacta 
0001 atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc 
0071 agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccaaccgggc 
0141 agcgatgctc ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactgcg 
0211 ggcacattaa cgctacttcc tgtcgcgcta cgtgcacctg tcactgcggg gcagttagcg gttgaaaatg 
0281 ggcattatgt ggtggaaacg ctggcgcgag cgtgcgatgg ttgtctgaac ggcgaatttg ccgcgctgat 
0351 cacaggtccg gtgcataaag gcgttattaa cgacgctggc attcctttta ccggtcatac cgagtttttc 
0421 gaagagcgtt cgcaggcgaa aaaggtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa 
0491 cgacgcattt accgctgcgc gatatcgcag acgctatcac ccctgcactt ttgcacgaag tgattgctat 
0561 tttgcatcac gatttgcgga ccaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg 
0631 cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat tccggtgctc aatgagctgc 
0701 gggcgcaggg gatgaaactc aacgggccgc tgcctgccga taccctgttt cagccgaaat atcttgataa 
0771 cgccgacgcc gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc 
0841 ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg 
0911 cgggacgtgg caaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa 
0981 cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg 
1051 atcagttcgt gatcgacagt attgtgtctg ccattaaccc