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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Related gene (MG1655 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
apaH
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Related strains
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Strains Search
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Search strains by gene name:
apaH
Search strains by all related name:
apaH b0049 cfcB ECK0050 JW0048
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General information (Go to Linear View: )
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Gene Name
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apaH
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Alternative name
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b0049,cfcB,ECK0050,JW0048
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Location, Length
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50,380 - 51,222
(
-
)
;
1.08
min
;
843
(bp) ,
280
(aa)
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Product
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diadenosine tetraphosphatase
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Note
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ECK0050:JW0048:b0049
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Function
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PID
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85674302
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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APAH_ECOLI
(
P05637
;
P78039
)
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- Protein name
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RecName: Full=Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]; EC=3.6.1.41; AltName: Full=Diadenosine tetraphosphatase; AltName: Full=Ap4A hydrolase; AltName: Full=Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase;
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- Synonyms
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- Gene name
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Name=apaH; OrderedLocusNames=b0049, JW0048;
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- Comments
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FUNCTION:Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP.
CATALYTIC ACTIVITY:P(1),P(4)-bis(5'-adenosyl) tetraphosphate + H(2)O = 2 ADP.
ENZYME REGULATION:Co(2+) is a strong stimulator (100-fold increase in rate of hydrolysis). Mn(2+), Cd(2+), Ni(2+), Mg(2+) and Ca(2+) are weak stimulators; the two latter act synergistically with Co(2+).
SUBUNIT:Monomer.
SIMILARITY:Belongs to the Ap4A hydrolase family.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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PDB
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PSI-BLAST
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SWISS-PROT
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BLAST
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RecName: Full=Bis
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PSI-BLAST
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nr
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BLAST
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diadenosine tetraphosphatase [Escherichia coli str. K-12 substr. MG1655]
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Pfam 27.0
(database updated :
2013-03
)
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Pfam
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MMBR References
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Mol Gen Genet. 1986;205(3):515-22.
The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA.
Blanchin-Roland S, Blanquet S, Schmitter JM, Fayat G.
(
3031429
)
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| Sequences |
Amino acid
FASTA format
|
0001 MATYLIGDVH GCYDELIALL HKVEFTPGKD TLWLTGDLVA RGPGSLDVLR YVKSLGDSVR LVLGNHDLHL
0071 LAVFAGISRN KPKDRLTPLL EAPDADELLN WLRRQPLLQI DEEKKLVMAH AGITPQWDLQ TAKECARDVE
0141 AVLSSDSYPF FLDAMYGDMP NNWSPELRGL GRLRFITNAF TRMRFCFPNG QLDMYSKESP EEAPAPLKPW
0211 FAIPGPVAEE YSIAFGHWAS LEGKGTPEGI YALDTGCCWG GTLTCLRWED KQYFVQPSNR HKDLGEAAAS
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gcagggtcac tacgaaatga tcgatgaaaa
-070 tggcgtccct ttcagcatcg acattcccgt attccgactc gccgttccca cactcattca ttaaaagaat
0001 atggcgacat accttattgg cgacgttcat ggttgttacg atgaactgat cgcattgctg cataaagtag
0071 aatttacccc tgggaaagat accctctggc tgacgggcga tctggtcgcg cgcgggccgg gttcgctgga
0141 tgttctgcgc tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga tctgcatctg
0211 ctggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac accgctgctg gaagcgccgg
0281 atgccgacga gctgcttaac tggctgcggc gccagcctct gctgcaaatc gacgaagaga aaaagctggt
0351 gatggcccac gcagggatca cgccgcagtg ggatctgcag accgccaaag agtgcgcacg cgatgtagaa
0421 gcggtgctat cgagtgactc ctatcccttc tttcttgatg ccatgtacgg cgatatgcca aataactggt
0491 caccggaatt gcgggggctg ggaagactgc gttttatcac caacgctttt acccgtatgc gtttttgctt
0561 cccgaacggt caactggata tgtacagcaa agaatcgccg gaagaggccc ctgccccact gaaaccgtgg
0631 tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca ctgggcatcg ctggagggca
0701 aaggtacgcc ggaaggtata tacgcgctgg ataccggctg ctgctggggt ggtacattaa cctgcctgcg
0771 ctgggaagat aaacagtatt ttgtccagcc gtcgaaccgg cataaggatt tgggcgaagc ggcggcgtct
0841 taaacacagc ctgatatagg aaggccggat aagacgcgac cggcgtcgca tccggcgcta gccgtaaatt
0911 ctatacaaaa ttaccgccgc tccagaatct caa
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