MG1655
W3110
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Gene Report : folA
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Can J Microbiol. 1999;45(3):191-200.
Growth properties of a folA null mutant of Escherichia coli K12.
Herrington MB, Chirwa NT. ( 10408091 )
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  folA
Related strains
     Strains Search Search strains by gene name:  folA   Search strains by all related name:  folA b0048 ECK0049 JW0047 tmrA

General information  (Go to Linear View:)
 Gene Name folA  
 Alternative name b0048,ECK0049,JW0047,tmrA  
 Location, Length 49,823 - 50,302 (  +  ) ;   1.07 min ; 480 (bp) ,   159 (aa) Go to Linear View
 Product dihydrofolate reductase  
 Note ECK0049:JW0047:b0048  
 Function  
 PID 21321929  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) DYR_ECOLI ( P0ABQ4 ; P00379 )
    -  Protein name RecName: Full=Dihydrofolate reductase; EC=1.5.1.3;  
    -  Synonyms  
    -  Gene name Name=folA; Synonyms=tmrA; OrderedLocusNames=b0048, JW0047;  
    -  Comments
  • CATALYTIC ACTIVITY:5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
  • PATHWAY:Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate:step 1/1.
  • MISCELLANEOUS:The strain K12 sequence is shown.
  • MISCELLANEOUS:Strain B [RT500] is resistant to 500 micrograms per milliliter of trimethoprim.
  • MISCELLANEOUS:Strain B [MB1428] is methotrexate-resistant.
  • MISCELLANEOUS:The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
  • SIMILARITY:Belongs to the dihydrofolate reductase family.
  • SIMILARITY:Contains 1 DHFR (dihydrofolate reductase) domain.  

    •  Linear View
    View Location
       40.0  –  65.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP  
        PDB
             PSI-BLAST Chain B, Neutron Crystal Structure Of Escherichia Coli Dihydrofolate Reductase Bound To The Anti-Cancer Drug, Methotrexate  
        SWISS-PROT
            BLAST  
            PSI-BLAST
        nr
             BLAST dihydrofolate reductase [Escherichia coli CFT073]  
    Pfam 27.0   (database updated : 2013-03 )
        Pfam

    Other Cross-Reference
        EcoCyc folA 

    Sequences
    Amino acid
    FASTA format 
    0001 MISLIAALAV DRVIGMENAM PWNLPADLAW FKRNTLNKPV IMGRHTWESI GRPLPGRKNI ILSSQPGTDD 
0071 RVTWVKSVDE AIAACGDVPE IMVIGGGRVY EQFLPKAQKL YLTHIDAEVE GDTHFPDYEP DDWESVFSEF 
0141 HDADAQNSHS YCFEILERR
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             AGCAGAATAT AAAATTTTCC TCAACATCAT 
-070 CCTCGCACCA GTCGACGACG GTTTACGCTT TACGTATAGT GGCGACAATT TTTTTTATCG GGAAATCTCA 
0001 atgatcagtc tgattgcggc gttagcggta gatcgcgtta tcggcatgga aaacgccatg ccgtggaacc 
0071 tgcctgccga tctcgcctgg tttaaacgca acaccttaaa taaacccgtg attatgggcc gccatacctg 
0141 ggaatcaatc ggtcgtccgt tgccaggacg caaaaatatt atcctcagca gtcaaccggg tacggacgat 
0211 cgcgtaacgt gggtgaagtc ggtggatgaa gccatcgcgg cgtgtggtga cgtaccagaa atcatggtga 
0281 ttggcggcgg tcgcgtttat gaacagttct tgccaaaagc gcaaaaactg tatctgacgc atatcgacgc 
0351 agaagtggaa ggcgacaccc atttcccgga ttacgagccg gatgactggg aatcggtatt cagcgaattc 
0421 cacgatgctg atgcgcagaa ctctcacagc tattgctttg agattctgga gcggcggtaa TTTTGTATAG 
0491 AATTTACGGC TAGCGCCGGA TGCGACGCCG GTCGCGTCTT ATCCGGCCTT CCTATATCAG GCTGTGTTTA 
0561 AGACGCCGCC GCTTCGCCCA