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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Related gene (MG1655 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
mtlA
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Related strains
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Strains Search
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Search strains by gene name:
mtlA
Search strains by all related name:
mtlA b3599 ECK3588 JW3573 mtlC
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General information (Go to Linear View: )
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Gene Name
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mtlA
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Alternative name
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b3599,ECK3588,JW3573,mtlC
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Location, Length
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3,866,221 - 3,868,134
(
-
)
;
83.21
min
;
1914
(bp) ,
637
(aa)
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Product
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fused mannitol-specific PTS enzyme IIABC components
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Note
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ECK3588:JW3573:b3599
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Function
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PID
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85676444
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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PTM3C_ECOLI
(
P00550
;
Q2M7R2
)
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- Protein name
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RecName: Full=PTS system mannitol-specific EIICBA component; AltName: Full=EIICBA-Mtl; Short=EII-Mtl; Includes: RecName: Full=Mannitol permease IIC component; AltName: Full=PTS system mannitol-specific EIIC component; Includes: RecName: Full=Mannitol-specific phosphotransferase enzyme IIB component; EC=2.7.1.69; AltName: Full=PTS system mannitol-specific EIIB component; Includes: RecName: Full=Mannitol-specific phosphotransferase enzyme IIA component; EC=2.7.1.-; AltName: Full=PTS system mannitol-specific EIIA component;
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- Synonyms
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- Gene name
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Name=mtlA; OrderedLocusNames=b3599, JW3573;
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- Comments
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FUNCTION:The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport.
CATALYTIC ACTIVITY:Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
CATALYTIC ACTIVITY:Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
SUBUNIT:Homodimer.
INTERACTION:P0A6F5:groL; NbExp=1; IntAct=EBI-547239, EBI-543750;
SUBCELLULAR LOCATION:Cell inner membrane; Multi-pass membrane protein.
DOMAIN:The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
DOMAIN:The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
DOMAIN:The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
PTM:An intramolecular phosphotransfer takes places between His-554 and Cys-384.
SIMILARITY:Contains 1 PTS EIIA type-2 domain.
SIMILARITY:Contains 1 PTS EIIB type-2 domain.
SIMILARITY:Contains 1 PTS EIIC type-2 domain.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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PDB
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PSI-BLAST
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SWISS-PROT
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BLAST
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PSI-BLAST
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nr
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BLAST
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fused mannitol-specific PTS enzymes: IIA components/IIB components/IIC components [Escherichia coli str. K-12 substr. MG1655]
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Pfam 27.0
(database updated :
2013-03
)
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Pfam
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MMBR References
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Mol Microbiol. 1988;2(3):405-12.
Nucleotide sequence of the mannitol (mtl) operon in Escherichia coli.
Davis T, Yamada M, Elgort M, Saier MH Jr.
(
3135464
)
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| Sequences |
Amino acid
FASTA format
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0001 MSSDIKIKVQ SFGRFLSNMV MPNIGAFIAW GIITALFIPT GWLPNETLAK LVGPMITYLL PLLIGYTGGK
0071 LVGGERGGVV GAITTMGVIV GADMPMFLGS MIAGPLGGWC IKHFDRWVDG KIKSGFEMLV NNFSAGIIGM
0141 ILAILAFLGI GPIVEALSKM LAAGVNFMVV HDMLPLASIF VEPAKILFLN NAINHGIFSP LGIQQSHELG
0211 KSIFFLIEAN PGPGMGVLLA YMFFGRGSAK QSAGGAAIIH FLGGIHEIYF PYVLMNPRLI LAVILGGMTG
0281 VFTLTILGGG LVSPASPGSI LAVLAMTPKG AYFANIAGVC AAMAVSFVVS AILLKTSKVK EEDDIEAATR
0351 RMQDMKAESK GASPLSAGDV TNDLSHVRKI IVACDAGMGS SAMGAGVLRK KIQDAGLSQI SVTNSAINNL
0421 PPDVDLVITH RDLTERAMRQ VPQAQHISLT NFLDSGLYTS LTERLVAAQR HTANEEKVKD SLKDSFDDSS
0491 ANLFKLGAEN IFLGRKAATK EEAIRFAGEQ LVKGGYVEPE YVQAMLDREK LTPTYLGESI AVPHGTVEAK
0561 DRVLKTGVVF CQYPEGVRFG EEEDDIARLV IGIAARNNEH IQVITSLTNA LDDESVIERL AHTTSVDEVL
0631 ELLAGRK
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 ttcgcagtat ctacaaggtc cggctacctc
-070 tgccgccaca ttaacaaaaa acctcgggct tccagcctgc gcgacagcaa acataagaag gggtgttttt
0001 atgtcatccg atattaagat caaagtgcaa agctttggtc gtttcctcag caacatggtg atgccaaata
0071 tcggcgcgtt tatcgcgtgg ggtatcatca ccgcgttatt tattccaaca gggtggttac cgaacgagac
0141 gctggcgaag ctggtcgggc cgatgatcac ttatctcctg ccgctgctga tcggttatac cggtggtaag
0211 ctggtaggcg gcgaacgtgg cggcgtagtc ggtgccatca ccaccatggg cgttatcgtc ggcgcagaca
0281 tgccgatgtt cctcggttct atgattgcag gtccgctggg cggctggtgc attaagcact tcgaccgctg
0351 ggtagacggt aagatcaaat ccggttttga gatgctggtg aataacttct ccgcaggcat catcgggatg
0421 atcctcgcta ttctggcatt cctcggcatt ggcccgattg ttgaagccct gtccaaaatg ctggctgcgg
0491 gcgttaactt catggttgtc catgacatgc tgccgctggc gtctatcttt gttgaaccgg cgaaaatcct
0561 gttcctcaac aacgccatta accacggtat cttctcgccg ctgggtattc agcagtccca tgaactgggt
0631 aaatcaatct tcttcctgat tgaagctaac ccaggtccag gtatgggcgt gctgctggcg tacatgttct
0701 ttggtcgtgg tagcgctaaa cagtctgcgg gcggtgcggc aatcatccac ttcctggggg gtatccacga
0771 aatctacttc ccgtatgtgc tgatgaatcc gcgtctgatc ctcgcagtca tcctcggcgg tatgactggc
0841 gtgttcacgc tgactatcct gggcggtggt ctggtttctc cggcatctcc gggttctatc cttgctgtac
0911 tggcgatgac accaaaaggt gcttacttcg ctaacatcgc gggtgtgtgt gcggcgatgg ctgtctcctt
0981 cgttgtctct gctattttgc tgaaaaccag caaagtgaaa gaagaagatg atattgaagc agcaactcgt
1051 cgtatgcagg acatgaaagc tgagtctaaa ggcgcatctc cgctgtctgc tggcgatgtg actaacgacc
1121 tgagccacgt acgtaaaatc atcgttgcct gtgacgccgg tatgggttcc agtgcgatgg gcgcaggcgt
1191 tctgcgtaag aaaattcagg atgcaggtct gtcgcagatt tctgttacta acagcgcgat caacaacctg
1261 ccgccagatg tggacctcgt catcactcac cgtgacctga ccgaacgcgc tatgcgccag gttccgcagg
1331 cacagcatat ttcgctgacc aacttcctcg acagcggcct gtacaccagc ctgaccgaac gtctggttgc
1401 tgcccaacgc cacacggcaa acgaagagaa agtaaaagac agcctgaaag acagctttga cgattccagt
1471 gctaacctgt tcaagctagg cgcggagaac atcttcctcg gtcgcaaagc ggcaaccaaa gaagaagcga
1541 ttcgttttgc tggcgagcag ctggtgaaag gcggttacgt tgagccggaa tacgttcagg cgatgctgga
1611 tcgtgaaaaa ctgaccccga cttatctggg tgagtctatc gcggtgccac acggtacggt tgaagcgaaa
1681 gatcgcgtac tgaaaacggg cgtcgtgttc tgccagtacc cggaaggcgt gcgcttcggt gaagaagaag
1751 atgacattgc ccgtctggtg attggtattg ctgcccgtaa caacgagcac attcaggtta tcaccagcct
1821 gaccaatgca ctggatgatg agtccgtcat cgagcgtctg gcacacacca ccagcgtgga tgaagtgctg
1891 gaactgctgg caggtcgtaa gtaatccaat cccaccctct ccacatggag aaggtggggt taattgcctg
1961 atgcgctacg cttatcaggc ctacaggatg catcacaatt tgttgaattt gcac
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