MG1655
W3110
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Gene Report : rbfA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  rbfA
Related strains
     Strains Search Search strains by gene name:  rbfA   Search strains by all related name:  rbfA b3167 ECK3156 JW3136 sdr-43 yhbB

General information  (Go to Linear View:)
 Gene Name rbfA  
 Alternative name b3167,ECK3156,JW3136,sdr-43,yhbB  
 Location, Length 3,312,632 - 3,313,033 (  -  ) ;   71.30 min ; 402 (bp) ,   133 (aa) Go to Linear View
 Product 30s ribosome binding factor  
 Note ECK3156:JW3136:b3167  
 Function  
 PID 85675963  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) RBFA_ECOLI ( P0A7G2 ; P09170; Q2M943 )
    -  Protein name RecName: Full=Ribosome-binding factor A; AltName: Full=Protein P15B;  
    -  Synonyms  
    -  Gene name Name=rbfA; Synonyms=P15B, yhbB; OrderedLocusNames=b3167, JW3136;  
    -  Comments
  • FUNCTION:Essential for efficient processing of 16S rRNA. Probably part of the 30S subunit prior to or during the final step in the processing of 16S free 30S ribosomal subunits. Could be some accessory protein needed for efficient assembly of the 30S subunit. May interact with the 5'-terminal helix region of 16S rRNA. Has affinity for free ribosomal 30S subunits but not for 70S ribosomes. Overexpression suppresses rimM deletion mutants.
  • INTERACTION:P18843:nadE; NbExp=1; IntAct=EBI-1131774, EBI-548960;
  • SUBCELLULAR LOCATION:Cytoplasm (Potential).
  • SIMILARITY:Belongs to the rbfA family.  

  •  Linear View
    View Location
       3300.0  –  3325.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP  
        PDB
             PSI-BLAST  
        SWISS-PROT
             BLAST RecName: Full=Ribosome-binding factor A; AltName: Full=Protein P15B  
             PSI-BLAST RecName: Full=Ribosome-binding factor A
        nr
             BLAST ribosome-binding factor A [Escherichia coli O157:H7 EDL933]  
    Pfam 27.0   (database updated : 2013-03 )
        Pfam

    Other Cross-Reference
        EcoCyc rbfA 

    MMBR References
    Genes Dev. 1995;9:626-637
    Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA.
    Dammel, C. S., and H. F. Noller.

    Sequences
    Amino acid
    FASTA format
    0001 MAKEFGRPQR VAQEMQKEIA LILQREIKDP RLGMMTTVSG VEMSRDLAYA KVYVTFLNDK DEDAVKAGIK 
    0071 ALQEASGFIR SLLGKAMRLR IVPELTFFYD NSLVEGMRMS NLVTSVVKHD EERRVNPDDS KED
    
    
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp
    -100                                             gacgctggcg cgtcttatca ggtcaacgtg 
    -070 aacagccctt ccacctgaaa ttaattttaa aaaggggcta acagcccctt ttttgtcagg agaatttatt 
    0001 atggcgaaag aatttggtcg cccgcagcgc gtagcgcagg aaatgcaaaa agagatcgct ctcatcctgc 
    0071 agcgtgaaat taaagatcct cgcctgggca tgatgaccac cgtttccggt gtcgaaatgt ctcgcgacct 
    0141 ggcgtatgcc aaagtatatg tgacgttcct caacgacaaa gatgaagacg cggttaaagc gggcatcaaa 
    0211 gcgttgcaag aagcttctgg tttcatccgc agcctgctgg ggaaagcgat gcgcctgcgt atcgtgccgg 
    0281 aactgacctt cttctacgac aactctctgg ttgaagggat gcgcatgtca aacctggtga ccagcgtggt 
    0351 caaacatgac gaagaacgtc gtgttaaccc ggacgacagc aaggaggact aatgagtcgt cctcgtcgtc 
    0421 gcggtcgcga cattaacggc gttttgttgc tggataaacc tcagggtatg tccagcaacg atgcgctgca 
    0491 aaaagtgaaa cg