MG1655
W3110
Search for
  Japanese | English
Top Genes Deletions Circular Linear Motif SequenceCutter Download Strains
Gene Report : hda
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
EMBO J. 2001;20(15):4253-62.
Hda, a novel DnaA-related protein, regulates the replication cycle in Escherichia coli.
Kato J, Katayama T. ( 11483528 )
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  hda
Related strains
     Strains Search Search strains by gene name:  hda   Search strains by all related name:  hda b2496 ECK2492 idaB JW5397 yfgE

General information  (Go to Linear View:)
 Gene Name hda  
 Alternative name b2496,ECK2492,idaB,JW5397,yfgE  
 Location, Length 2,616,731 - 2,617,477 (  -  ) ;   56.32 min ; 747 (bp) ,   248 (aa) Go to Linear View
 Product ATPase regulatory factor involved in DnaA inactivation  
 Note ECK2492:JW5397:b2496  
 Function  
 PID 85675427  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) HDA_ECOLI ( P69931 ; P76570; P76979 )
    -  Protein name RecName: Full=DnaA-homolog protein hda;  
    -  Synonyms  
    -  Gene name Name=hda; Synonyms=yfgE; OrderedLocusNames=b2496, JW5397;  
    -  Comments
  • FUNCTION:Mediates the interactions of dnaA with the beta subunit sliding clamp, promoting hydrolysis of dnaA-bound ATP. It is essential for the control of initiation of DNA replication by inhibiting reinitiation of replication. ADP-binding activates hda to hydrolyze dnaA-ATP; monomers bind to ADP with about 200-fold greater affinity than for ATP.
  • SUBUNIT:The active form seems to be an ADP-bound monomer; apo-hda form homo-multimers that do not hydrolzye dnaA-bound ATP. Forms a large complex with ATP-dnaA, ADP-hda and the DNA-loaded clamp.
  • INTERACTION:P0A988:dnaN; NbExp=1; IntAct=EBI-545453, EBI-542385;
  • SIMILARITY:Belongs to the dnaA family. HdA subfamily.  

    •  Linear View
    View Location
       2605.0  –  2630.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP  
        PDB
             PSI-BLAST  
        SWISS-PROT
            BLAST  
            PSI-BLAST
        nr
             BLAST DNA replication initiation factor [Escherichia coli O157:H7 EDL933]  
    Pfam 27.0   (database updated : 2013-03 )
        Pfam

    Other Cross-Reference
        EcoCyc hda 

    Sequences
    Amino acid
    FASTA format 
    0001 MVNFSRFCEI LVEVSLNTPA QLSLPLYLPD DETFASFWPG DNSSLLAALQ NVLRQEHSGY IYLWAREGAG 
0071 RSHLLHAACA ELSQRGDAVG YVPLDKRTWF VPEVLDGMEH LSLVCIDNIE CIAGDELWEM AIFDLYNRIL 
0141 ESGKTRLLIT GDRPPRQLNL GLPDLASRLD WGQIYKLQPL SDEDKLQALQ LRARLRGFEL PEDVGRFLLK 
0211 RLDREMRTLF MTLDQLDRAS ITAQRKLTIP FVKEILKL
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             cctgaagaag tggtgctgga cgcagaagac 
-070 gcggatataa cagacaaata atgtaccgac cgggcagtgt tgctgcccgg ttcaaacatc atgggattct 
0001 gtggtaaact tctcgcgatt ttgtgaaatc ctggttgagg tatctctgaa cacaccggca cagctctctt 
0071 tgccacttta tcttcctgac gacgaaacct ttgcaagttt ctggccgggg gataactcct ctttactggc 
0141 cgcgctgcaa aacgtgctgc gtcaggaaca tagcggttac atctatctct gggcacgcga aggcgcgggg 
0211 cgcagccatc tgctgcacgc ggcttgcgcg gaattgtcgc agcgtggcga tgcggtgggc tatgtcccgc 
0281 tggataaacg cacctggttt gttccggaag tgctcgacgg tatggagcat ttgtcgctgg tctgtatcga 
0351 caacattgag tgtattgcag gcgatgagtt gtgggagatg gcgattttcg atctctacaa tcgaattctg 
0421 gaatcgggca aaacacggtt gttgatcacc ggcgatcgtc caccgcggca gttgaatctg ggattaccgg 
0491 atctcgcgtc gcgactcgac tgggggcaga tctacaaatt gcagccactt tctgatgaag ataagttgca 
0561 ggcgctacag ttacgcgcgc gtttgcgtgg ttttgaactg ccggaagatg tggggcgttt cttgctgaag 
0631 cggctcgaca gagaaatgcg cacgctattt atgacgttgg atcagttgga tcgtgcgtcg attaccgcgc 
0701 aacgtaagct gaccattccg tttgtgaaag aaattctgaa gttgtagata aggtgtttat tgtcggatgc 
0771 ggcgcgaacg ccttatccga actacgcgaa gttaatccat agtattcgta ggccggataa gacgtggcaa 
0841 gcgtcgc