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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Related gene (MG1655 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
ypdD
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Related strains
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Strains Search
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Search strains by gene name:
ypdD
Search strains by all related name:
ypdD b2383 ECK2379 f831 fryA JW2380
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General information (Go to Linear View: )
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Gene Name
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ypdD
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Alternative name
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b2383,ECK2379,f831,fryA,JW2380
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Location, Length
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2,507,436 - 2,509,931
(
-
)
;
53.97
min
;
2496
(bp) ,
831
(aa)
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Product
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fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component
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Note
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ECK2379:JW2380:b2383
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Function
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PID
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1799794
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SWISS-PROT
(
Show simple
)
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Entry name(Acc.no)
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PTFX1_ECOLI
(
P77439
)
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- Protein name
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RecName: Full=Multiphosphoryl transfer protein 1; Short=MTP 1; Includes: RecName: Full=Phosphoenolpyruvate-protein phosphotransferase; EC=2.7.3.9; AltName: Full=Phosphotransferase system enzyme I; Includes: RecName: Full=Phosphocarrier protein HPr; Short=Protein H; Includes: RecName: Full=Fructose-like phosphotransferase enzyme IIA component 2; EC=2.7.1.-; AltName: Full=PTS system fructose-like EIIA component 2;
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- Synonyms
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- Gene name
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Name=fryA; Synonyms=ypdD; OrderedLocusNames=b2383, JW2380;
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- Comments
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FUNCTION:Multifunctional protein that includes general (non sugar-specific) and sugar-specific components of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr). HPr transfers the phosphoryl group to the phosphoryl carrier EIIA, which then transfers it to EIIB (By similarity).
CATALYTIC ACTIVITY:Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.
CATALYTIC ACTIVITY:Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
COFACTOR:Magnesium.
INTERACTION:P0AFG8:aceE; NbExp=1; IntAct=EBI-545399, EBI-542683;
SUBCELLULAR LOCATION:Cytoplasm (Probable).
DOMAIN:The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
DOMAIN:The Enzyme I (EI) N-terminal domain contains the HPr binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site (By similarity).
MISCELLANEOUS:The Enzyme I (EI) reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain (By similarity).
SIMILARITY:Belongs to the PEP-utilizing enzyme family.
SIMILARITY:Contains 1 HPr domain.
SIMILARITY:Contains 1 PTS EIIA type-2 domain.
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| Sequences |
Amino acid
FASTA format
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0001 MLTIQFLCPL PNGLHARPAW ELKEQCSQWQ SEITFINHRQ NAKADAKSSL ALIGTGTLFN DSCSLNISGS
0071 DEEQARRVLE EYIQVRFIDS DSVQPTQAEL TAHPLPRSLS RLNPDLLYGN VLASGVGVGT LTLLQSDSLD
0141 SYRAIPASAQ DSTRLEHSLA TLAEQLNQQL RERDGESKTI LSAHLSLIQD DEFAGNIRRL MTEQHQGLGA
0211 AIISNMEQVC AKLSASASDY LRERVSDIRD ISEQLLHITW PELKPRNKLV LEKPTILVAE DLTPSQFLSL
0281 DLKNLAGMIL EKTGRTSHTL ILARASAIPV LSGLPLDAIA RYAGQPAVLD AQCGVLAINP NDAVSGYYQV
0351 AQTLADKRQK QQAQAAAQLA YSRDNKRIDI AANIGTALEA PGAFANGAEG VGLFRTEMLY MDRDSAPDEQ
0421 EQFEAYQQVL LAAGDKPIIF RTMDIGGDKS IPYLNIPQEE NPFLGYRAVR IYPEFAGLFR TQLRAILRAA
0491 SFGNAQLMIP MVHSLDQILW VKGEIQKAIV ELKRDGLRHA ETITLGIMVE VPSVCYIIDH FCDEVDFFSI
0561 GSNDMTQYLY AVDRNNPRVS PLYNPITPSF LRMLQQIVTT AHQRGKWVGI CGELGGESRY LPLLLGLGLD
0631 ELSMSSPRIP AVKSQLRQLD SEACRELARQ ACECRSAQEI EALLTAFTPE EDVRPLLALE NIFVDQDFSN
0701 KEQAIQFLCG NLGVNGRTEH PFELEEDVWQ REEIVTTGVG FGVAIPHTKS QWIRHSSISI ARLAKPIGWQ
0771 SEMGEVELVI MLTLGANEGM NHVKVFSQLA RKLVNKNFRQ SLFAAQDAQS ILTLLETELT F
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 gatgcagcaa cttttatctg cccttattca
-070 acgtcttacg cgtgagacgg ttgttcaact gacggatttc agatgatctc ctgattaacc cggagcggtt
0001 atgttaacga ttcaatttct ctgtcctttg cctaacggtc tacatgctcg tccggcgtgg gaacttaaag
0071 aacagtgcag ccagtggcaa agcgaaatca cttttattaa ccatcgccag aacgcaaagg cagatgcgaa
0141 aagctcgctg gcgctgattg gcaccggcac cctatttaat gacagttgca gcctgaacat tagcggcagc
0211 gatgaagagc aggcgcggcg cgtgctggaa gagtacatcc aggtgcgctt tatcgacagc gacagcgttc
0281 agcctacgca ggcagaactg acggcgcatc cgctgccgcg ttcattaagc cgccttaacc cggatttact
0351 gtacggcaat gtgctggcaa gcggcgtcgg cgtgggtacg ctgaccctgt tacaaagcga cagcctcgac
0421 agttatcggg caatccccgc cagtgcgcaa gattccaccc ggctggagca cagcctggca acgcttgccg
0491 agcaactgaa ccagcaattg cgtgagcgtg acggcgaaag caaaactatc ctcagcgccc atttgtcgct
0561 gattcaggat gatgaatttg caggcaatat ccgtcgcctg atgacagaac agcatcaggg gctgggggcg
0631 gcgatcatca gcaatatgga gcaggtttgc gccaaacttt ctgcctctgc cagcgattat ctgcgcgaac
0701 gtgttagcga cattcgcgat atcagcgaac agttgctgca tatcacctgg ccggaactga agccgcgcaa
0771 caagctggtg cttgaaaaac cgaccattct ggtggctgaa gatttaaccc caagccagtt tttgagcctt
0841 gatttgaaaa atcttgcggg catgattctg gagaaaaccg ggcgcacctc gcatacactg attctggccc
0911 gtgcctcggc gatcccggta ctgagtggct tgccgctgga tgcgattgcc cgttatgccg ggcaacctgc
0981 agtgcttgac gcccagtgcg gcgtgctggc gattaacccg aatgacgcgg tgagcggtta ttatcaggtc
1051 gcgcagacgc tggcggataa acgccaaaaa caacaggcgc aggctgccgc gcagctggcc tattcccgtg
1121 ataacaagcg tattgatatt gcggcgaata tcggcaccgc tctggaagcg ccaggcgcgt ttgccaacgg
1191 cgcggaaggt gtcgggctgt tccgtaccga aatgctctat atggatcgcg acagcgcgcc ggacgagcag
1261 gagcaatttg aagcctacca gcaggtgcta ctggcggcgg gcgacaagcc gattatcttc cgcacgatgg
1331 acatcggcgg cgataaaagc attccttatc tgaatattcc ccaggaagag aacccgttcc tcggctatcg
1401 cgcggtacgt atttacccgg aatttgctgg cctgttccgc actcaactgc gggccatttt gcgcgccgcc
1471 agtttcggca acgcccagtt gatgatcccg atggttcaca gcctcgatca gatcttatgg gtgaaaggcg
1541 agatccaaaa agcgatcgtt gagcttaagc gcgatggcct gcgtcatgca gagacgatta cgcttgggat
1611 catggtggaa gttccgtcgg tgtgctacat catcgaccac ttctgcgatg aggtcgattt cttcagtatc
1681 ggctccaacg atatgaccca gtatctgtat gcggtcgatc gtaataaccc gcgcgtatcg ccgctatata
1751 acccgattac gccatcgttc ctgcgcatgt tgcagcaaat agttaccact gcgcatcagc ggggcaaatg
1821 ggtaggcatt tgcggtgaac tgggcggtga aagccgttat ctgccgctac tgcttgggct gggcctggat
1891 gagctgagta tgagtagccc gcgtattccg gcggtgaaaa gccagcttcg tcaactggat agcgaggcgt
1961 gtcgggaact ggcgcgtcag gcatgtgaat gccgcagtgc gcaggaaatt gaagcgttac tcaccgcctt
2031 tacgccggaa gaagacgttc gcccactgct ggcgctggag aatatctttg ttgatcagga ttttagcaat
2101 aaagagcagg cgatccagtt cctgtgcggc aacctcggcg ttaacgggcg cactgaacat ccgtttgagc
2171 tggaagaaga tgtctggcag cgggaagaga ttgttaccac cggcgttggt tttggcgtag cgatcccgca
2241 caccaaatct cagtggatcc gtcattccag tatcagcatt gcccggctgg cgaaaccgat tggctggcag
2311 tcagaaatgg gcgaagtcga actggtgatc atgctgacgc tgggtgctaa cgaagggatg aatcatgtga
2381 aagtcttctc gcagctggcg cgtaaactgg tgaataaaaa cttccgccag tcgttgtttg ccgcgcaaga
2451 tgcacaaagt atcctgacgc tgctggaaac agaattaacc ttctgacgtt agccctgaaa acgggcgctg
2521 tactctcccg gcgtcagacc aaactgacgc cggaaaacgc gacaaaaata gtcgctatcc ggaaaaccgc
2591 aacgct
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