MG1655
W3110
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Gene Report : thrA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  thrA
Related strains
     Strains Search Search strains by gene name:  thrA   Search strains by all related name:  thrA b0002 ECK0002 Hs JW0001 thrA1 thrA2 thrD

General information  (Go to Linear View:)
 Gene Name thrA  
 Alternative name b0002,ECK0002,Hs,JW0001,thrA1,thrA2,thrD  
 Location, Length 337 - 2,799 (  +  ) ;   0.01 min ; 2463 (bp) ,   820 (aa) Go to Linear View
 Product fused aspartokinase I and homoserine dehydrogenase I  
 Note ECK0002:JW0001:b0002  
 Function  
 PID 85674276  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) AK1H_ECOLI ( P00561 ; Q47659; Q6LEL0 )
    -  Protein name RecName: Full=Bifunctional aspartokinase/homoserine dehydrogenase 1; AltName: Full=Aspartokinase I/homoserine dehydrogenase I; Short=AKI-HDI; Includes: RecName: Full=Aspartokinase; EC=2.7.2.4; Includes: RecName: Full=Homoserine dehydrogenase; EC=1.1.1.3;  
    -  Synonyms  
    -  Gene name Name=thrA; Synonyms=thrA1, thrA2; OrderedLocusNames=b0002, JW0001;  
    -  Comments
  • CATALYTIC ACTIVITY:L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H.
  • CATALYTIC ACTIVITY:ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
  • ENZYME REGULATION:The enzyme activities are regulated allosterically by L-threonine.
  • PATHWAY:Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate:step 1/4.
  • PATHWAY:Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate:step 1/3.
  • PATHWAY:Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate:step 3/3.
  • PATHWAY:Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate:step 1/5.
  • PATHWAY:Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate:step 3/5.
  • SUBUNIT:Homotetramer.
  • MISCELLANEOUS:Aspartokinase II-homoserine dehydrogenase II and aspartokinase III also catalyze the same reaction(s).
  • SIMILARITY:In the N-terminal section; belongs to the aspartokinase family.
  • SIMILARITY:In the C-terminal section; belongs to the homoserine dehydrogenase family.
  • SIMILARITY:Contains 2 ACT domains.  

    •  Linear View
    View Location
       4625.0  –  25.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP  
        PDB
             PSI-BLAST  
        SWISS-PROT
             BLAST RecName: Full=Bifunctional aspartokinase/homoserine dehydrogenase 1; AltName: Full=Aspartokinase I/homoserine dehydrogenase I; Short=AKI-HDI; Includes: RecName: Full=Aspartokinase; Includes: RecName: Full=Homoserine dehydrogenase  
            PSI-BLAST
        nr
             BLAST fused aspartokinase I and homoserine dehydrogenase I [Escherichia coli str. K-12 substr. MG1655]  
    Pfam 27.0   (database updated : 2013-03 )
        Pfam

    Other Cross-Reference
        EcoCyc thrA 

    MMBR References
    Mol Gen Genet. 1982;188(2):225-7.
    Attenuation in the threonine operon: effects of amino acids present in the presumed leader peptide in addition to threonine and isoleucine.
    Daniel J, Saint-Girons I. ( 6818426 )

    Sequences
    Amino acid
    FASTA format 
    0001 MRVLKFGGTS VANAERFLRV ADILESNARQ GQVATVLSAP AKITNHLVAM IEKTISGQDA LPNISDAERI 
0071 FAELLTGLAA AQPGFPLAQL KTFVDQEFAQ IKHVLHGISL LGQCPDSINA ALICRGEKMS IAIMAGVLEA 
0141 RGHNVTVIDP VEKLLAVGHY LESTVDIAES TRRIAASRIP ADHMVLMAGF TAGNEKGELV VLGRNGSDYS 
0211 AAVLAACLRA DCCEIWTDVD GVYTCDPRQV PDARLLKSMS YQEAMELSYF GAKVLHPRTI TPIAQFQIPC 
0281 LIKNTGNPQA PGTLIGASRD EDELPVKGIS NLNNMAMFSV SGPGMKGMVG MAARVFAAMS RARISVVLIT 
0351 QSSSEYSISF CVPQSDCVRA ERAMQEEFYL ELKEGLLEPL AVTERLAIIS VVGDGMRTLR GISAKFFAAL 
0421 ARANINIVAI AQGSSERSIS VVVNNDDATT GVRVTHQMLF NTDQVIEVFV IGVGGVGGAL LEQLKRQQSW 
0491 LKNKHIDLRV CGVANSKALL TNVHGLNLEN WQEELAQAKE PFNLGRLIRL VKEYHLLNPV IVDCTSSQAV 
0561 ADQYADFLRE GFHVVTPNKK ANTSSMDYYH QLRYAAEKSR RKFLYDTNVG AGLPVIENLQ NLLNAGDELM 
0631 KFSGILSGSL SYIFGKLDEG MSFSEATTLA REMGYTEPDP RDDLSGMDVA RKLLILARET GRELELADIE 
0701 IEPVLPAEFN AEGDVAAFMA NLSQLDDLFA ARVAKARDEG KVLRYVGNID EDGVCRVKIA EVDGNDPLFK 
0771 VKNGENALAF YSHYYQPLPL VLRGYGAGND VTAAGVFADL LRTLSWKLGV
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             AGGTAACGGT GCGGGCTGAC GCGTACAGGA 
-070 AACACAGAAA AAAGCCCGCA CCTGACAGTG CGGGCTTTTT TTTTCGACCA AAGGTAACGA GGTAACAACC 
0001 atgcgagtgt tgaagttcgg cggtacatca gtggcaaatg cagaacgttt tctgcgtgtt gccgatattc 
0071 tggaaagcaa tgccaggcag gggcaggtgg ccaccgtcct ctctgccccc gccaaaatca ccaaccacct 
0141 ggtggcgatg attgaaaaaa ccattagcgg ccaggatgct ttacccaata tcagcgatgc cgaacgtatt 
0211 tttgccgaac ttttgacggg actcgccgcc gcccagccgg ggttcccgct ggcgcaattg aaaactttcg 
0281 tcgatcagga atttgcccaa ataaaacatg tcctgcatgg cattagtttg ttggggcagt gcccggatag 
0351 catcaacgct gcgctgattt gccgtggcga gaaaatgtcg atcgccatta tggccggcgt attagaagcg 
0421 cgcggtcaca acgttactgt tatcgatccg gtcgaaaaac tgctggcagt ggggcattac ctcgaatcta 
0491 ccgtcgatat tgctgagtcc acccgccgta ttgcggcaag ccgcattccg gctgatcaca tggtgctgat 
0561 ggcaggtttc accgccggta atgaaaaagg cgaactggtg gtgcttggac gcaacggttc cgactactct 
0631 gctgcggtgc tggctgcctg tttacgcgcc gattgttgcg agatttggac ggacgttgac ggggtctata 
0701 cctgcgaccc gcgtcaggtg cccgatgcga ggttgttgaa gtcgatgtcc taccaggaag cgatggagct 
0771 ttcctacttc ggcgctaaag ttcttcaccc ccgcaccatt acccccatcg cccagttcca gatcccttgc 
0841 ctgattaaaa ataccggaaa tcctcaagca ccaggtacgc tcattggtgc cagccgtgat gaagacgaat 
0911 taccggtcaa gggcatttcc aatctgaata acatggcaat gttcagcgtt tctggtccgg ggatgaaagg 
0981 gatggtcggc atggcggcgc gcgtctttgc agcgatgtca cgcgcccgta tttccgtggt gctgattacg 
1051 caatcatctt ccgaatacag catcagtttc tgcgttccac aaagcgactg tgtgcgagct gaacgggcaa 
1121 tgcaggaaga gttctacctg gaactgaaag aaggcttact ggagccgctg gcagtgacgg aacggctggc 
1191 cattatctcg gtggtaggtg atggtatgcg caccttgcgt gggatctcgg cgaaattctt tgccgcactg 
1261 gcccgcgcca atatcaacat tgtcgccatt gctcagggat cttctgaacg ctcaatctct gtcgtggtaa 
1331 ataacgatga tgcgaccact ggcgtgcgcg ttactcatca gatgctgttc aataccgatc aggttatcga 
1401 agtgtttgtg attggcgtcg gtggcgttgg cggtgcgctg ctggagcaac tgaagcgtca gcaaagctgg 
1471 ctgaagaata aacatatcga cttacgtgtc tgcggtgttg ccaactcgaa ggctctgctc accaatgtac 
1541 atggccttaa tctggaaaac tggcaggaag aactggcgca agccaaagag ccgtttaatc tcgggcgctt 
1611 aattcgcctc gtgaaagaat atcatctgct gaacccggtc attgttgact gcacttccag ccaggcagtg 
1681 gcggatcaat atgccgactt cctgcgcgaa ggtttccacg ttgtcacgcc gaacaaaaag gccaacacct 
1751 cgtcgatgga ttactaccat cagttgcgtt atgcggcgga aaaatcgcgg cgtaaattcc tctatgacac 
1821 caacgttggg gctggattac cggttattga gaacctgcaa aatctgctca atgcaggtga tgaattgatg 
1891 aagttctccg gcattctttc tggttcgctt tcttatatct tcggcaagtt agacgaaggc atgagtttct 
1961 ccgaggcgac cacgctggcg cgggaaatgg gttataccga accggacccg cgagatgatc tttctggtat 
2031 ggatgtggcg cgtaaactat tgattctcgc tcgtgaaacg ggacgtgaac tggagctggc ggatattgaa 
2101 attgaacctg tgctgcccgc agagtttaac gccgagggtg atgttgccgc ttttatggcg aatctgtcac 
2171 aactcgacga tctctttgcc gcgcgcgtgg cgaaggcccg tgatgaagga aaagttttgc gctatgttgg 
2241 caatattgat gaagatggcg tctgccgcgt gaagattgcc gaagtggatg gtaatgatcc gctgttcaaa 
2311 gtgaaaaatg gcgaaaacgc cctggccttc tatagccact attatcagcc gctgccgttg gtactgcgcg 
2381 gatatggtgc gggcaatgac gttacagctg ccggtgtctt tgctgatctg ctacgtaccc tctcatggaa 
2451 gttaggagtc tgaCATGGTT AAAGTTTATG CCCCGGCTTC CAGTGCCAAT ATGAGCGTCG GGTTTGATGT 
2521 GCTCGGGGCG GCGGTGACAC CTGTTGATGG TGCATTGCTC GGA