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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Related gene (MG1655 PEC)
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|
Gene Search
|
Search MG1655 PEC by gene name:
chbB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
chbB
Search strains by all related name:
chbB b1738 celA ECK1736 JW1727
|
General information (Go to Linear View: )
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Gene Name
|
chbB
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|
Alternative name
|
b1738,celA,ECK1736,JW1727
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|
Location, Length
|
1,823,013 - 1,823,333
(
-
)
;
39.24
min
;
321
(bp) ,
106
(aa)
|
|
Product
|
N,N'-diacetylchitobiose-specific enzyme IIB component of PTS
|
|
Note
|
ECK1736:JW1727:b1738
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Function
|
|
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PID
|
1742835
|
|
SWISS-PROT
(
Show simple
)
|
Entry name(Acc.no)
|
PTQB_ECOLI
(
P69795
;
P17409
)
|
|
- Protein name
|
RecName: Full=N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component; EC=2.7.1.69; AltName: Full=PTS system N,N'-diacetylchitobiose-specific EIIB component;
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- Synonyms
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- Gene name
|
Name=chbB; Synonyms=celA; OrderedLocusNames=b1738, JW1727;
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|
- Comments
|
FUNCTION:The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport.
CATALYTIC ACTIVITY:Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
SUBUNIT:Monomer; in both its unphosphorylated and phosphorylated forms.
SUBCELLULAR LOCATION:Cytoplasm.
INDUCTION:By N,N'-diacetylchitobiose.
DOMAIN:The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
SIMILARITY:Contains 1 PTS EIIB type-3 domain.
CAUTION:Was originally (PubMed:2092358) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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PDB
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PSI-BLAST
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SWISS-PROT
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BLAST
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PSI-BLAST
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nr
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BLAST
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N,N'-diacetylchitobiose-specific PTS system transporter subunit IIB [Escherichia coli O157:H7 EDL933]
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Pfam 27.0
(database updated :
2013-03
)
|
|
Pfam
|
|
| Sequences |
Amino acid
FASTA format
|
0001 MEKKHIYLFC SAGMSTSLLV SKMRAQAEKY EVPVIIEAFP ETLAGEKGQN ADVVLLGPQI AYMLPEIQRL
0071 LPNKPVEVID SLLYGKVDGL GVLKAAVAAI KKAAAN
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 aaaaaagtca tgttggtttc gcaagttacc
-070 cagagcatgg aagcaggtta aggcttgcgg agtgtctggc tgacagataa tcgtcgatga gggcagtttt
0001 atggaaaaga aacacattta tctgttttgt tctgcgggca tgtctacctc tttactggta tcaaaaatgc
0071 gcgcacaggc agaaaaatat gaagttccgg tcattattga agcatttccg gaaacactgg ctggtgaaaa
0141 aggtcagaat gccgatgtcg tgttattagg gccgcagatt gcttatatgt tgcccgaaat ccagcgtttg
0211 ttacccaaca aaccggttga agtaattgac tcgctgcttt atggcaaagt cgatggttta ggcgtgctta
0281 aggctgcggt tgcagcgatt aaaaaagccg cagcaaatta atttatttta aattttcccg tcaaagagtt
0351 atttcataaa tcaataccgc aatatttaaa ttgcggtttt taagggtatt tttctatgag taatgttatt
0421 g
|
