MG1655
W3110
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Gene Report : dnaK
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  dnaK
Related strains
     Strains Search Search strains by gene name:  dnaK   Search strains by all related name:  dnaK b0014 ECK0014 groPAB groPC groPF grpC grpF JW0013 seg

General information  (Go to Linear View:)
 Gene Name dnaK  
 Alternative name b0014,ECK0014,groPAB,groPC,groPF,grpC,grpF,JW0013,seg  
 Location, Length 12,163 - 14,079 (  +  ) ;   0.26 min ; 1917 (bp) ,   638 (aa) Go to Linear View
 Product chaperone Hsp70, co-chaperone with DnaJ  
 Note ECK0014:JW0013:b0014  
 Function  
 PID 21321902  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) DNAK_ECOLI ( P0A6Y8 ; P04475 )
    -  Protein name RecName: Full=Chaperone protein dnaK; AltName: Full=Heat shock protein 70; AltName: Full=Heat shock 70 kDa protein; AltName: Full=HSP70;  
    -  Synonyms  
    -  Gene name Name=dnaK; Synonyms=groP, grpF, seg; OrderedLocusNames=b0014, JW0013;  
    -  Comments
  • FUNCTION:Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the dnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the dnaA protein. Also participates actively in the response to hyperosmotic shock.
  • INTERACTION:P37685:aldB; NbExp=1; IntAct=EBI-542092, EBI-1132062; P00811:ampC; NbExp=1; IntAct=EBI-542092, EBI-1134803; P0A9Q9:asd; NbExp=1; IntAct=EBI-542092, EBI-1132825; P0AC38:aspA; NbExp=1; IntAct=EBI-542092, EBI-544200; P16700:cysP; NbExp=1; IntAct=EBI-542092, EBI-560567; P0C0V0:degP; NbExp=1; IntAct=EBI-542092, EBI-547165; P08622:dnaJ; NbExp=1; IntAct=EBI-542092, EBI-545285; P0AAI5:fabF; NbExp=1; IntAct=EBI-542092, EBI-542783; P0AB71:fbaA; NbExp=1; IntAct=EBI-542092, EBI-370916; P10346:glnQ; NbExp=1; IntAct=EBI-542092, EBI-1120383; P0AC81:gloA; NbExp=1; IntAct=EBI-542092, EBI-551143; P37024:hrpB; NbExp=1; IntAct=EBI-542092, EBI-549226; P0A6Z3:htpG; NbExp=1; IntAct=EBI-542092, EBI-369221; P62623:ispH; NbExp=1; IntAct=EBI-542092, EBI-554022; P45578:luxS; NbExp=1; IntAct=EBI-542092, EBI-562313; P28635:metQ; NbExp=1; IntAct=EBI-542092, EBI-1114713; P12282:moeB; NbExp=1; IntAct=EBI-542092, EBI-554435; P30011:nadC; NbExp=1; IntAct=EBI-542092, EBI-551015; P17117:nfsA; NbExp=1; IntAct=EBI-542092, EBI-1120624; P69924:nrdB; NbExp=1; IntAct=EBI-542092, EBI-555196; P00634:phoA; NbExp=1; IntAct=EBI-542092, EBI-552958; P28304:qor; NbExp=1; IntAct=EBI-542092, EBI-556687; P21513:rne; NbExp=1; IntAct=EBI-542092, EBI-549958; P68919:rplY; NbExp=1; IntAct=EBI-542092, EBI-553087; P0ADX9:rsmD; NbExp=1; IntAct=EBI-542092, EBI-561207; P23721:serC; NbExp=1; IntAct=EBI-542092, EBI-557952; P0A853:tnaA; NbExp=1; IntAct=EBI-542092, EBI-371316; P0AG80:ugpB; NbExp=1; IntAct=EBI-542092, EBI-546637; P69822:ulaB; NbExp=1; IntAct=EBI-542092, EBI-1113922; P76002:ycgK; NbExp=1; IntAct=EBI-542092, EBI-1122554; P0ACX3:ydhR; NbExp=1; IntAct=EBI-542092, EBI-544817; Q46906:ygcP; NbExp=1; IntAct=EBI-542092, EBI-557727; Q46796:ygeP; NbExp=1; IntAct=EBI-542092, EBI-1130615; P36677:yhdN; NbExp=1; IntAct=EBI-542092, EBI-1124572; P46853:yhhX; NbExp=1; IntAct=EBI-542092, EBI-1132804; P37342:yjjI; NbExp=1; IntAct=EBI-542092, EBI-548519;
  • SUBCELLULAR LOCATION:Cytoplasm. Cell inner membrane; Peripheral membrane protein.
  • PTM:Autophosphorylated; grpE inhibits the autophosphorylation.
  • SIMILARITY:Belongs to the heat shock protein 70 family.  

    •  Linear View
    View Location
       0.0  –  25.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP  
        PDB
             PSI-BLAST  
        SWISS-PROT
             BLAST RecName: Full=Chaperone protein dnaK; AltName: Full=Heat shock protein 70; AltName: Full=Heat shock 70 kDa protein; AltName: Full=HSP70  
             PSI-BLAST RecName: Full=Chaperone protein dnaK; AltName: Full=Heat shock protein 70; AltName: Full=Heat shock 70 kDa protein; AltName: Full=HSP70
        nr
             BLAST hypothetical protein EscherichiacoliO157_19612 [Escherichia coli O157:H7 str. FRIK2000]  
    Pfam 27.0   (database updated : 2013-03 )
        Pfam

    Other Cross-Reference
        EcoCyc dnaK 

    MMBR References
    Proc Natl Acad Sci U S A. 1996;93:10632-10637
    Mutations in the C-terminal fragment of DnaK affecting peptide binding.
    Burkholder, W. F., X. Zhao, X. Zhu, W. A. Hendrickson, A. Gragerov, and M. E. Gottesman.

    Sequences
    Amino acid
    FASTA format 
    0001 MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK 
0071 RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI 
0141 TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV 
0211 DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD 
0281 VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ 
0351 KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTGDVKDVL LLDVTPLSLG IETMGGVMTT LIAKNTTIPT 
0421 KHSQVFSTAE DNQSAVTIHV LQGERKRAAD NKSLGQFNLD GINPAPRGMP QIEVTFDIDA DGILHVSAKD 
0491 KNSGKEQKIT IKASSGLNED EIQKMVRDAE ANAEADRKFE ELVQTRNQGD HLLHSTRKQV EEAGDKLPAD 
0561 DKTAIESALT ALETALKGED KAAIEAKMQE LAQVSQKLME IAQQQHAQQQ TAGADASANN AKDDDVVDAE 
0631 FEEVKDKK
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             GTCTGCAAAA AAATGAAATT GGGCAGTTGA 
-070 AACCAGACGT TTCGCCCCTA TTACAGACTC ACAACCACAT GATGACCGAA TATATAGTGG AGACGTTTAG 
0001 atgggtaaaa taattggtat cgacctgggt actaccaact cttgtgtagc gattatggat ggcaccactc 
0071 ctcgcgtgct ggagaacgcc gaaggcgatc gcaccacgcc ttctatcatt gcctataccc aggatggtga 
0141 aactctagtt ggtcagccgg ctaaacgtca ggcagtgacg aacccgcaaa acactctgtt tgcgattaaa 
0211 cgcctgattg gtcgccgctt ccaggacgaa gaagtacagc gtgatgtttc catcatgccg ttcaaaatta 
0281 ttgctgctga taacggcgac gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc agatttctgc 
0351 tgaagtgctg aaaaaaatga agaaaaccgc tgaagattac ctgggtgaac cggtaactga agctgttatc 
0421 accgtaccgg catactttaa cgatgctcag cgtcaggcaa ccaaagacgc aggccgtatc gctggtctgg 
0491 aagtaaaacg tatcatcaac gaaccgaccg cagctgcgct ggcttacggt ctggacaaag gcactggcaa 
0561 ccgtactatc gcggtttatg acctgggtgg tggtactttc gatatttcta ttatcgaaat cgacgaagtt 
0631 gacggcgaaa aaaccttcga agttctggca accaacggtg atacccacct ggggggtgaa gacttcgaca 
0701 gccgtctgat caactatctg gttgaagaat tcaagaaaga tcagggcatt gacctgcgca acgatccgct 
0771 ggcaatgcag cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt cttccgctca gcagaccgac 
0841 gttaacctgc catacatcac tgcagacgcg accggtccga aacacatgaa catcaaagtg actcgtgcga 
0911 aactggaaag cctggttgaa gatctggtaa accgttccat tgagccgctg aaagttgcac tgcaggacgc 
0981 tggcctgtcc gtatctgata tcgacgacgt tatcctcgtt ggtggtcaga ctcgtatgcc aatggttcag 
1051 aagaaagttg ctgagttctt tggtaaagag ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg 
1121 gtgctgctgt tcagggtggt gttctgactg gtgacgtaaa agacgtactg ctgctggacg ttaccccgct 
1191 gtctctgggt atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga aaaacaccac tatcccgacc 
1261 aagcacagcc aggtgttctc taccgctgaa gacaaccagt ctgcggtaac catccatgtg ctgcagggtg 
1331 aacgtaaacg tgcggctgat aacaaatctc tgggtcagtt caacctagat ggtatcaacc cggcaccgcg 
1401 cggcatgccg cagatcgaag ttaccttcga tatcgatgct gacggtatcc tgcacgtttc cgcgaaagat 
1471 aaaaacagcg gtaaagagca gaagatcacc atcaaggctt cttctggtct gaacgaagat gaaatccaga 
1541 aaatggtacg cgacgcagaa gctaacgccg aagctgaccg taagtttgaa gagctggtac agactcgcaa 
1611 ccagggcgac catctgctgc acagcacccg taagcaggtt gaagaagcag gcgacaaact gccggctgac 
1681 gacaaaactg ctatcgagtc tgcgctgact gcactggaaa ctgctctgaa aggtgaagac aaagccgcta 
1751 tcgaagcgaa aatgcaggaa ctggcacagg tttcccagaa actgatggaa atcgcccagc agcaacatgc 
1821 ccagcagcag actgccggtg ctgatgcttc tgcaaacaac gcgaaagatg acgatgttgt cgacgctgaa 
1891 tttgaagaag tcaaagacaa aaaataaTCG CCCTATAAAC GGGTAATTAT ACTGACACGG GCGAAGGGGA 
1961 ATTTCCTCTC CGCCCGTGCA TTCATCTAGG GGCAATTTAA AAAAGATGGC TAAGCAA