MG1655
W3110
Search for
  Japanese | English
Top Genes Deletions Circular Linear Motif BLAST SequenceCutter Download Strains
Gene Report : murD
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)  
Related gene (MG1655 PEC)
     Gene Search Search MG1655 PEC by gene name:  murD
Related strains
     Strains Search Search strains by gene name:  murD   Search strains by all related name:  murD b0088 ECK0089 JW0086

General information  (Go to Linear View:)
 Gene Name murD  
 Alternative name b0088,ECK0089,JW0086  
 Location, Length 97,087 - 98,403 (  +  ) ;   2.09 min ; 1317 (bp) ,   438 (aa) Go to Linear View
 Product UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase  
 Note ECK0089:JW0086:b0088  
 Function  
 PID 21321969  
SWISS-PROT  ( Show simple )
  botton Entry name(Acc.no) MURD_ECOLI ( P14900 )
    -  Protein name RecName: Full=UDP-N-acetylmuramoylalanine--D-glutamate ligase; EC=6.3.2.9; AltName: Full=UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; AltName: Full=D-glutamic acid-adding enzyme;  
    -  Synonyms  
    -  Gene name Name=murD; OrderedLocusNames=b0088, JW0086;  
    -  Comments
  • FUNCTION:Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).
  • CATALYTIC ACTIVITY:ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate.
  • PATHWAY:Cell wall biogenesis; peptidoglycan biosynthesis.
  • INTERACTION:P0A9K9:slyD; NbExp=1; IntAct=EBI-554780, EBI-369251; P0AGG4:trxC; NbExp=1; IntAct=EBI-554780, EBI-549392;
  • SUBCELLULAR LOCATION:Cytoplasm.
  • SIMILARITY:Belongs to the murCDEF family.  

    •  Linear View
    View Location
       85.0  –  110.0 (KBP)

    Homology Analysis
    BLAST
        Bacteria
             GTOP  
        PDB
             PSI-BLAST  
        SWISS-PROT
             BLAST RecName: Full=UDP-N-acetylmuramoylalanine--D-glutamate ligase; AltName: Full=UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; AltName: Full=D-glutamic acid-adding enzyme  
            PSI-BLAST
        nr
             BLAST UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase [Escherichia coli str. K-12 substr. MG1655]  
    Pfam 27.0   (database updated : 2013-03 )
        Pfam

    Other Cross-Reference
        EcoCyc murD 

    MMBR References
    Eur J Biochem. 1991;202:1169-1176
    Overproduction, purification and properties of the uridine diphsophate N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli.
    Pratviel-Sosa, F., D. Mengin-Lecreulx, and J. van Heijenoort.

    Sequences
    Amino acid
    FASTA format 
    0001 MADYQGKNVV IIGLGLTGLS CVDFFLARGV TPRVMDTRMT PPGLDKLPEA VERHTGSLND EWLMAADLIV 
0071 ASPGIALAHP SLSAAADAGI EIVGDIELFC REAQAPIVAI TGSNGKSTVT TLVGEMAKAA GVNVGVGGNI 
0141 GLPALMLLDD ECELYVLELS SFQLETTSSL QAVAATILNV TEDHMDRYPF GLQQYRAAKL RIYENAKVCV 
0211 VNADDALTMP IRGADERCVS FGVNMGDYHL NHQQGETWLR VKGEKVLNVK EMKLSGQHNY TNALAALALA 
0281 DAAGLPRASS LKALTTFTGL PHRFEVVLEH NGVRWINDSK ATNVGSTEAA LNGLHVDGTL HLLLGGDGKS 
0351 ADFSPLARYL NGDNVRLYCF GRDGAQLAAL RPEVAEQTET MEQAMRLLAP RVQPGDMVLL SPACASLDQF 
0421 KNFEQRGNEF ARLAKELG
    Nucleotide
    FASTA format

    View sequence out neighbor 100bp 
    -100                                             AACTGAAAGG CTGGCCGGAA CCGCGCGTCA 
-070 TTGTGCGTTT CTGGATTATT TCGCTGATGC TGGTTCTGAT TGGTCTGGCA ACGCTGAAGG TACGTTAATC 
0001 atggctgatt atcagggtaa aaatgtcgtc attatcggcc tgggcctcac cgggctttcc tgcgtggact 
0071 ttttcctcgc tcgcggtgtg acgccgcgcg ttatggatac gcgtatgaca ccgcctggcc tggataaatt 
0141 acccgaagcc gtagaacgcc acacgggcag tctgaatgat gaatggctga tggcggcaga tctgattgtc 
0211 gccagtcccg gtattgcact ggcgcatcca tccttaagcg ctgccgctga tgccggaatc gaaatcgttg 
0281 gcgatatcga gctgttctgt cgcgaagcac aagcaccgat tgtggcgatt accggttcta acggcaaaag 
0351 cacggtcacc acgctagtgg gtgaaatggc gaaagcggcg ggggttaacg ttggtgtggg tggcaatatt 
0421 ggcctgcctg cgttgatgct actggatgat gagtgtgaac tgtacgtgct ggaactgtcg agcttccagc 
0491 tggaaaccac ctccagctta caggcggtag cagcgaccat tctgaacgtg actgaagatc atatggatcg 
0561 ctatccgttt ggtttacaac agtatcgtgc agcaaaactg cgcatttacg aaaacgcgaa agtttgcgtg 
0631 gttaatgctg atgatgcctt aacaatgccg attcgcggtg cggatgaacg ctgcgtcagc tttggcgtca 
0701 acatgggtga ctatcacctg aatcatcagc agggcgaaac ctggctgcgg gttaaaggcg agaaagtgct 
0771 gaatgtgaaa gagatgaaac tttccgggca gcataactac accaatgcgc tggcggcgct ggcgctggca 
0841 gatgctgcag ggttaccgcg tgccagcagc ctgaaagcgt taaccacatt cactggtctg ccgcatcgct 
0911 ttgaagttgt gctggagcat aacggcgtac gttggattaa cgattcgaaa gcgaccaacg tcggcagtac 
0981 ggaagcggcg ctgaatggcc tgcacgtaga cggcacactg catttgttgc tgggtggcga tggtaaatcg 
1051 gcggacttta gcccactggc gcgttacctg aatggcgata acgtacgtct gtattgtttc ggtcgtgacg 
1121 gcgcgcagct ggcggcgcta cgcccggaag tggcagaaca aaccgaaact atggaacagg cgatgcgctt 
1191 gctggctccg cgtgttcagc cgggcgatat ggttctgctc tccccagcct gtgccagcct tgatcagttc 
1261 aagaactttg aacaacgagg caatgagttt gcccgtctgg cgaaggagtt aggttgaTGC GTTTATCTCT 
1331 CCCTCGCCTG AAAATGCCGC GCCTGCCAGG ATTCAGTATC CTGGTCTGGA TCTCCACGGC GCTAAAGGGC 
1401 TGGGTGATGG GCTCGCG