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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
15
(LD)
,
15+dps
(LD)
,
OCR48,50-8 (LD 15D::Cm)
(D)
,
OCR49
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
poxB
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Related strains
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Strains Search
|
Search strains by gene name:
poxB
Search strains by all related name:
poxB b0871 ECK0862 JW0855
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General information (Go to Linear View: )
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Gene Name
|
poxB
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Alternative name
|
b0871,ECK0862,JW0855
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Location, Length
|
908,554 - 910,272
(
-
)
;
19.58
min
;
1719
(bp) ,
572
(aa)
|
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Product
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pyruvate dehydrogenase (pyruvate oxidase), thiamin-dependent, FAD-binding
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Operon Name
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poxB-ltaE-ybjT
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Note
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pyruvate oxidase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0042867 - pyruvate catabolic process
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Function
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enzyme; Degradation of small molecules: Carbon compounds
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Gene Ontology
|
GO:0000287
;
magnesium ion binding ( poxB )
GO:0003824
;
catalytic activity ( poxB )
GO:0005515
;
protein binding ( poxB )
GO:0005886
;
plasma membrane ( poxB )
GO:0008289
;
lipid binding ( poxB )
GO:0008985
;
pyruvate dehydrogenase (cytochrome) activity ( poxB )
GO:0016020
;
membrane ( poxB )
GO:0016491
;
oxidoreductase activity ( poxB )
GO:0016740
;
transferase activity ( poxB )
GO:0030976
;
thiamin pyrophosphate binding ( poxB )
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PID
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1787096
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EC number
(KEGG Pathway)
|
1.2.5.1
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
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poxB
(
EG10754
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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POXB_ECOLI
(
Q47520
)
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- Protein name
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Pyruvate dehydrogenase [cytochrome]
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- Synonyms
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EC 1.2.2.2, Pyruvate oxidase, POX, Pyruvate dehydrogenase [Ubiquinone]
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- Gene name
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Name=poxB; OrderedLocusNames=b0871;
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MMBR References
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J Bacteriol. 1984;160(3):1088-92.
Molecular cloning of the gene (poxB) encoding the pyruvate oxidase of Escherichia coli, a lipid-activated enzyme.
Grabau C, Cronan JE Jr.
(
6209262
)
|
Nucleic Acids Res. 1986;14:5449-5460
Nucleotide sequence and deduced amino acid sequence of Escherichia coli pyruvate oxidase, a lipid-activated flavoprotein.
Grabau, C., and J. E. Cronan, Jr.
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Mol Microbiol. 1994;11(6):1019-28.
Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene.
Chang YY, Wang AY, Cronan JE Jr.
(
8022274
)
|
J Bacteriol. 1983;154(2):756-62.
Genetic and biochemical analyses of Escherichia coli strains having a mutation in the structural gene (poxB) for pyruvate oxidase.
Chang YY, Cronan JE Jr.
(
6341362
)
|
J Bacteriol. 1991;173(17):5258-9.
Locations of the lip, poxB, and ilvBN genes on the physical map of Escherichia coli.
Chang YY, Cronan JE Jr, Li SJ, Reed K, Vanden Boom T, Wang AY.
(
1832150
)
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| Sequences |
Amino acid
FASTA format
|
0001 MKQTVAAYIA KTLESAGVKR IWGVTGDSLN GLSDSLNRMG TIEWMSTRHE EVAAFAAGAE AQLSGELAVC
0071 AGSCGPGNLH LINGLFDCHR NHVPVLAIAA HIPSSEIGSG YFQETHPQEL FRECSHYCEL VSSPEQIPQV
0141 LAIAMRKAVL NRGVSVVVLP GDVALKPAPE GATMHWYHAP QPVVTPEEEE LRKLAQLLRY SSNIALMCGS
0211 GCAGAHKELV EFAGKIKAPI VHALRGKEHV EYDNPYDVGM TGLIGFSSGF HTMMNADTLV LLGTQFPYRA
0281 FYPTDAKIIQ IDINPASIGA HSKVDMALVG DIKSTLRALL PLVEEKADRK FLDKALEDYR DARKGLDDLA
0351 KPSEKAIHPQ YLAQQISHFA ADDAIFTCDV GTPTVWAARY LKMNGKRRLL GSFNHGSMAN AMPQALGAQA
0421 TEPERQVVAM CGDGGFSMLM GDFLSVVQMK LPVKIVVFNN SVLGFVAMEM KAGGYLTDGT ELHDTNFARI
0491 AEACGITGIR VEKASEVDEA LQRAFSIDGP VLVDVVVAKE ELAIPPQIKL EQAKGFSLYM LRAIISGRGD
0561 EVIELAKTNW LR
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ggctatttaa ccgttagtgc ctcctttctc
-070 tcccatccct tccccctccg tcagatgaac taaacttgtt accgttatca cattcaggag atggagaacc
0001 atgaaacaaa cggttgcagc ttatatcgcc aaaacactcg aatcggcagg ggtgaaacgc atctggggag
0071 tcacaggcga ctctctgaac ggtcttagtg acagtcttaa tcgcatgggc accatcgagt ggatgtccac
0141 ccgccacgaa gaagtggcgg cctttgccgc tggcgctgaa gcacaactta gcggagaact ggcggtctgc
0211 gccggatcgt gcggccccgg caacctgcac ttaatcaacg gcctgttcga ttgccaccgc aatcacgttc
0281 cggtactggc gattgccgct catattccct ccagcgaaat tggcagcggc tatttccagg aaacccaccc
0351 acaagagcta ttccgcgaat gtagtcacta ttgcgagctg gtttccagcc cggagcagat cccacaagta
0421 ctggcgattg ccatgcgcaa agcggtgctt aaccgtggcg tttcggttgt cgtgttacca ggcgacgtgg
0491 cgttaaaacc tgcgccagaa ggggcaacca tgcactggta tcatgcgcca caaccagtcg tgacgccgga
0561 agaagaagag ttacgcaaac tggcgcaact gctgcgttat tccagcaata tcgccctgat gtgtggcagc
0631 ggctgcgcgg gggcgcataa agagttagtt gagtttgccg ggaaaattaa agcgcctatt gttcatgccc
0701 tgcgcggtaa agaacatgtc gaatacgata atccgtatga tgttggaatg accgggttaa tcggcttctc
0771 gtcaggtttc cataccatga tgaacgccga cacgttagtg ctactcggca cgcaatttcc ctaccgcgcc
0841 ttctacccga ccgatgccaa aatcattcag attgatatca acccagccag catcggcgct cacagcaagg
0911 tggatatggc actggtcggc gatatcaagt cgactctgcg tgcattgctt ccattggtgg aagaaaaagc
0981 cgatcgcaag tttctggata aagcgctgga agattaccgc gacgcccgca aagggctgga cgatttagct
1051 aaaccgagcg agaaagccat tcacccgcaa tatctggcgc agcaaattag tcattttgcc gccgatgacg
1121 ctattttcac ctgtgacgtt ggtacgccaa cggtgtgggc ggcacgttat ctaaaaatga acggcaagcg
1191 tcgcctgtta ggttcgttta accacggttc gatggctaac gccatgccgc aggcgctggg tgcgcaggcg
1261 acagagccag aacgtcaggt ggtcgccatg tgcggcgatg gcggttttag catgttgatg ggcgatttcc
1331 tctcagtagt gcagatgaaa ctgccagtga aaattgtcgt ctttaacaac agcgtgctgg gctttgtggc
1401 gatggagatg aaagctggtg gctatttgac tgacggcacc gaactacacg acacaaactt tgcccgcatt
1471 gccgaagcgt gcggcattac gggtatccgt gtagaaaaag cgtctgaagt tgatgaagcc ctgcaacgcg
1541 ccttctccat cgacggtccg gtgttggtgg atgtggtggt cgccaaagaa gagttagcca ttccaccgca
1611 gatcaaactc gaacaggcca aaggtttcag cctgtatatg ctgcgcgcaa tcatcagcgg acgcggtgat
1681 gaagtgatcg aactggcgaa aacaaactgg ctaaggtaaa aagggtggca tttcccgtca taataaggac
1751 atgccatgat tgatttacgc agtgataccg ttacccgacc aagccgcgcc atgctcgaag cgatgatgg
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