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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(ompT-fepC)266
,
OCR44,45-2 (LD 12-1_P/O)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
fepA
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Related strains
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Strains Search
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Search strains by gene name:
fepA
Search strains by all related name:
fepA b0584 cbr cbt ECK0576 fep feuB JW5086
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General information (Go to Linear View: )
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Gene Name
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fepA
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Alternative name
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b0584,cbr,cbt,ECK0576,fep,feuB,JW5086
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Location, Length
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609,477 - 611,717
(
-
)
;
13.14
min
;
2241
(bp) ,
746
(aa)
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Product
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iron-enterobactin outer membrane transporter
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Operon Name
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fepA-entD
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Note
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outer membrane receptor for ferric enterobactin (enterochelin) and colicins B and D; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0009279 - cell outer membrane; GO_process: GO:0006826 - iron ion transport
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Function
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membrane; Transport of small molecules: Cations
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Gene Ontology
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GO:0004872
;
receptor activity ( fepA )
GO:0005215
;
transporter activity ( fepA )
GO:0005506
;
iron ion binding ( fepA )
GO:0006810
;
transport ( fepA )
GO:0006811
;
ion transport ( fepA )
GO:0006826
;
iron ion transport ( fepA )
GO:0009279
;
cell outer membrane ( fepA )
GO:0015343
;
siderophore-iron transmembrane transporter activity ( fepA )
GO:0015891
;
siderophore transport ( fepA )
GO:0016020
;
membrane ( fepA )
GO:0016021
;
integral to membrane ( fepA )
GO:0019867
;
outer membrane ( fepA )
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PID
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1786798
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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fepA
(
EG10293
)
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Number of removed
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22 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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FEPA_ECOLI
(
P05825
;
P75722; P76821; P77093
)
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- Protein name
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Ferrienterobactin receptor precursor
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- Synonyms
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Enterobactin outer-membrane receptor
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- Gene name
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Name=fepA; Synonyms=fep, feuB; OrderedLocusNames=b0584;
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MMBR References
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Gene. 1985;34(1):47-54.
Physical and genetic characterization of cloned enterobactin genomic sequences from Escherichia coli K-12.
Fleming TP, Nahlik MS, Neilands JB, McIntosh MA.
(
3159622
)
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J Bacteriol. 1994;176:3944-3955
Promoter and operator determinants for Fur-mediated iron regulation in the bidirectional fepA-fes control region of the Escherichia coli enterobactin gene system.
Hunt, M. D., G. S. Pettis, and M. A. McIntosh.
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J Biol Chem. 1986;261:10797-10801
Nucleotide sequence of the gene for the ferrienterochelin receptor fepA in Escherichia coli: homology among outer membrane receptors which interact with TonB.
Lundrigan, M. D., and R. J. Kadner.
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J Bacteriol. 1987;169(8):3638-46.
Genetic organization of multiple fep genes encoding ferric enterobactin transport functions in Escherichia coli.
Ozenberger BA, Nahlik MS, McIntosh MA.
(
2956250
)
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J Biol Chem. 1988;263:18857-18863
Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter.
Pettis, G. S., R. J. Brickman, and M. A. McIntosh.
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J Bacteriol. 1983;155(1):330-6.
Two fep genes are required for ferrienterochelin uptake in Escherichia coli K-12.
Pierce JR, Pickett CL, Earhart CF.
(
6223021
)
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J Bacteriol. 1976;126(3):1052-62.
Iron uptake in colicin B-resistant mutants of Escherichia coli K-12.
Pugsley AP, Reeves P.
(
7543
)
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Mol Microbiol. 1991;5(6):1415-28.
Nucleotide sequence and genetic organization of the ferric enterobactin transport system: homology to other periplasmic binding protein-dependent systems in Escherichia coli.
Shea CM, McIntosh MA.
(
1838574
)
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J Bacteriol. 1978;133(2):661-6.
Involvement of inner and outer membrane components in the transport of iron and in colicin B action in Escherichia coli.
Wookey P, Rosenberg H.
(
146699
)
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FEMS Microbiol Lett. 1984;25:111-116.
Characterization of a plasmid carrying the Escherichia coli K-12 entD, fepA, fes and entF genes.
Coderre, P. E., and C. F. Earhart.
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| Sequences |
Amino acid
FASTA format
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0001 MNKKIHSLAL LVNLGIYGVA QAQEPTDTPV SHDDTIVVTA AEQNLQAPGV STITADEIRK NPVARDVSKI
0071 IRTMPGVNLT GNSTSGQRGN NRQIDIRGMG PENTLILIDG KPVSSRNSVR QGWRGERDTR GDTSWVPPEM
0141 IERIEVLRGP AAARYGNGAA GGVVNIITKK GSGEWHGSWD AYFNAPEHKE EGATKRTNFS LTGPLGDEFS
0211 FRLYGNLDKT QADAWDINQG HQSARAGTYA TTLPAGREGV INKDINGVVR WDFAPLQSLE LEAGYSRQGN
0281 LYAGDTQNTN SDSYTRSKYG DETNRLYRQN YALTWNGGWD NGVTTSNWVQ YEHTRNSRIP EGLAGGTEGK
0351 FNEKATQDFV DIDLDDVMLH SEVNLPIDFL VNQTLTLGTE WNQQRMKDLS SNTQALTGTN TGGAIDGVST
0421 TDRSPYSKAE IFSLFAENNM ELTDSTIVTP GLRFDHHSIV GNNWSPALNI SQGLGDDFTL KMGIARAYKA
0491 PSLYQTNPNY ILYSKGQGCY ASAGGCYLQG NDDLKAETSI NKEIGLEFKR DGWLAGVTWF RNDYRNKIEA
0561 GYVAVGQNAV GTDLYQWDNV PKAVVEGLEG SLNVPVSETV MWTNNITYML KSENKTTGDR LSIIPEYTLN
0631 STLSWQARED LSMQTTFTWY GKQQPKKYNY KGQPAVGPET KEISPYSIVG LSATWDVTKN VSLTGGVDNL
0701 FDKRLWRAGN AQTTGDLAGA NYIAGAGAYT YNEPGRTWYM SVNTHF
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 tctttcagga tcaaaggttt tcgcggtagc
-070 gggatgcgtc gtgttgatga cgaccatgcc cgacagttgc aattcgtggc aaaaatgcag gaataaaaca
0001 atgaacaaga agattcattc cctggccttg ttggtcaatc tggggattta tggggtagcg caggcacaag
0071 agccgaccga tactcctgtt tcacatgacg atactattgt cgttaccgcc gccgagcaga acttacaggc
0141 gcctggcgtt tcgaccatca ccgcagatga aatccgcaaa aacccggttg cccgcgatgt gtcgaagatc
0211 atccgtacca tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
0281 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa gcagccgtaa
0351 ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatactt cctgggtgcc acctgaaatg
0421 attgaacgta ttgaagttct gcgtggtccg gcagctgcgc gttatggcaa cggcgcggcg ggcggcgtgg
0491 ttaacatcat taccaaaaaa ggcagcggcg agtggcacgg ctcctgggac gcatatttca atgcgccaga
0561 acataaagag gaaggtgcca ccaaacgcac taactttagc ctgaccggtc cgctgggcga cgaattcagc
0631 ttccgtttgt atggcaacct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
0701 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggta atcaacaaag atattaatgg
0771 cgtggtgcgc tgggatttcg cgccattgca atcgctggaa ctggaagcag gttacagccg ccagggtaac
0841 ctgtatgcgg gcgacaccca gaataccaac tccgattcct atacccgctc gaaatatggc gatgaaacca
0911 accgtctgta tcgccagaac tacgcgctga cctggaacgg tggctgggat aacggcgtga ccaccagcaa
0981 ctgggtgcag tacgaacaca cccgtaactc gcgtattccg gaaggtctgg cgggcggtac cgaagggaaa
1051 tttaacgaaa aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
1121 acctgccgat tgatttcctc gttaaccaga cgctgacgct gggtacggag tggaatcagc aacggatgaa
1191 ggacttaagt tccaacaccc aggcactgac cggaacgaat accggtggcg ctattgatgg cgtgagtacc
1261 accgaccgta gcccgtattc aaaagcagaa attttctcgc tgtttgccga aaacaacatg gagctgactg
1331 acagcaccat cgtaacgccg gggctgcgtt tcgatcatca cagtattgtc ggcaataact ggagcccggc
1401 gctgaacata tcgcaaggtt taggcgatga cttcacgctg aaaatgggca tcgcccgtgc ttataaagcg
1471 ccgagcctgt accagactaa cccgaactac attctctaca gtaaaggtca gggttgctat gccagcgcgg
1541 gcggctgcta tctgcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga ttggtctgga
1611 gttcaaacgc gacgggtggc tggcgggcgt cacctggttc cgtaacgatt atcgcaataa gattgaagcg
1681 ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc tctatcagtg ggataacgtg ccgaaagcgg
1751 tggttgaagg tctggaagga tcgttaaacg taccggttag cgaaacggtg atgtggacca ataacatcac
1821 ttatatgctg aagagtgaaa acaaaaccac gggcgaccgt ttgtcgatca tcccggagta tacgttgaac
1891 tcaacgctga gctggcaggc acgggaagat ttgtcgatgc aaacgacctt cacctggtac ggcaagcagc
1961 agccgaagaa gtacaactat aaaggtcagc cagcggttgg accggaaacc aaagaaatta gtccttacag
2031 cattgttggc ctgagcgcga cctgggatgt gacgaagaat gtcagtctga ccggcggcgt ggacaatctg
2101 ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca cgggcgattt ggcaggggcc aactatatcg
2171 ccggtgccgg ggcgtatacc tataacgagc cgggacgtac gtggtatatg agcgtaaaca cccacttctg
2241 atgctaacgt cagattgttg acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc cttatccggc
2311 ctacaaaaat cttgccaatt caatatattg c
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