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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
J Bacteriol. 1999;181(24):7479-84.
Genetic analysis of a chromosomal region containing genes required for assimilation of allantoin nitrogen and linked glyoxylate metabolism in Escherichia coli.
Cusa E, Obradors N, Baldoma L, Badia J, Aguilar J.
(
10601204
)
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Deletion
|
28
(LD)
,
OCR42,43-3 (LD 28B_CRS)
(D)
,
OCR42,43-4 (LD 28B_P/O)
(D)
,
OCR43
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
gcl
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Related strains
|
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Strains Search
|
Search strains by gene name:
gcl
Search strains by all related name:
gcl b0507 ECK0500 JW0495
|
General information (Go to Linear View: )
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Gene Name
|
gcl
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Alternative name
|
b0507,ECK0500,JW0495
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Location, Length
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533,140 - 534,921
(
+
)
;
11.49
min
;
1782
(bp) ,
593
(aa)
|
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Product
|
glyoxylate carboligase
|
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Operon Name
|
gcl-hyi-glxR-ybbVW-allB-ybbY-glxK
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Note
|
GO_process: GO:0009441 - glycolate metabolic process; GO_process: GO:0009442 - allantoin assimilation pathway
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Function
|
enzyme; Central intermediary metabolism: Pool, multipurpose conversions
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Gene Ontology
|
GO:0000287
;
magnesium ion binding ( gcl )
GO:0003824
;
catalytic activity ( gcl )
GO:0009028
;
tartronate-semialdehyde synthase activity ( gcl )
GO:0009436
;
glyoxylate catabolic process ( gcl )
GO:0016829
;
lyase activity ( gcl )
GO:0030976
;
thiamin pyrophosphate binding ( gcl )
|
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PID
|
1786717
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EC number
(KEGG Pathway)
|
4.1.1.47
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
gcl
(
EG11583
)
|
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Number of removed
|
1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
|
GCL_ECOLI
(
P30146
;
P77126
)
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- Protein name
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Glyoxylate carboligase
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- Synonyms
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EC 4.1.1.47, Tartronate-semialdehyde synthase
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- Gene name
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Name=gcl; OrderedLocusNames=b0507, z0661, ECs0568;
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MMBR References
|
J Biol Chem. 1993;268:3911-3919
Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase. An enzyme of the acetohydroxy acid synthase-pyruvate oxidase family.
Chang, Y.-Y., A. Y. Wang, and J. E. Cronan, Jr.
|
| Sequences |
Amino acid
FASTA format
|
0001 MAKMRAVDAA MYVLEKEGIT TAFGVPGAAI NPFYSAMRKH GGIRHILARH VEGASHMAEG YTRATAGNIG
0071 VCLGTSGPAG TDMITALYSA SADSIPILCI TGQAPRARLH KEDFQAVDIE AIAKPVSKMA VTVREAALVP
0141 RVLQQAFHLM RSGRPGPVLV DLPFDVQVAE IEFDPDMYEP LPVYKPAASR MQIEKAVEML IQAERPVIVA
0211 GGGVINADAA ALLQQFAELT SVPVIPTLMG WGCIPDDHEL MAGMVGLQTA HRYGNATLLA SDMVFGIGNR
0281 FANRHTGSVE KYTEGRKIVH IDIEPTQIGR VLCPDLGIVS DAKAALTLLV EVAQEMQKAG RLPCRKEWVA
0351 DCQQRKRTLL RKTHFDNVPV KPQRVYEEMN KAFGRDVCYV TTIGLSQIAA AQMLHVFKDR HWINCGQAGP
0421 LGWTIPAALG VCAADPKRNV VAISGDFDFQ FLIEELAVGA QFNIPYIHVL VNNAYLGLIR QSQRAFDMDY
0491 CVQLAFENIN SSEVNGYGVD HVKVAEGLGC KAIRVFKPED IAPAFEQAKA LMAQYRVPVV VEVILERVTN
0561 ISMGSELDNV MEFEDIADNA ADAPTETCFM HYE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CACATCCATA ATGTCTGTCG CATCCCGCTC
-070 TGCGGAGCGG GTTTTTTTGA CAAAATTTGA AAGTTGGAAA AATTTTCCAA TAAATAGAGG TAGGAATAAA
0001 atggcaaaaa tgagagccgt tgacgcggca atgtatgtgc tggagaaaga aggtatcact accgccttcg
0071 gtgttccggg agctgcaatc aatccgttct actcagcgat gcgtaagcac ggcggtattc gtcacattct
0141 ggcgcgtcat gtggaaggtg cttcgcacat ggcggaaggt tatacccgcg caacggcagg gaatatcggc
0211 gtatgtctgg ggacttccgg tcctgcgggc acggacatga tcaccgcgct ctattccgct tctgctgatt
0281 ccattcctat tctgtgcatt accggccagg caccgcgcgc ccgtctgcat aaagaagatt ttcaggccgt
0351 agatattgaa gcaattgcta aaccggtcag caaaatggcg gttacagttc gtgaagcggc gctggtgcct
0421 cgcgtgctgc aacaggcatt tcacctgatg cgttctggtc gtccgggtcc ggtactggtg gatttaccgt
0491 tcgacgttca ggttgcggaa atcgagtttg atcctgacat gtacgaaccg ctgccggtct acaaacctgc
0561 tgccagccgt atgcagatcg aaaaagctgt agaaatgtta atccaggccg aacgtccggt gattgttgcc
0631 gggggcgggg taattaatgc tgacgcagct gcactgttac aacagtttgc tgaactgacc agcgttccgg
0701 tgatcccaac gctaatgggc tggggctgta tcccggacga tcatgaactg atggccggga tggtgggtct
0771 gcaaaccgcg catcgttacg gtaacgcaac gctgctggcg tctgacatgg tgtttggtat cggtaaccgt
0841 tttgctaacc gtcataccgg ctcggtagag aaatacaccg aagggcgcaa aatcgttcat attgatattg
0911 agccgacgca aattggtcgc gtgctgtgtc cggatctcgg tattgtctct gatgctaaag cggcgctgac
0981 actgctggtt gaagtggcgc aggagatgca aaaagcgggt cgtctgccgt gtcgtaaaga atgggtcgcc
1051 gactgccagc agcgtaaacg cactttgctg cgcaaaaccc acttcgacaa cgtgccggtg aaaccgcagc
1121 gcgtgtatga agagatgaac aaagcctttg gtcgcgatgt ttgttatgtc accaccattg gtctgtcaca
1191 aatcgctgcg gcacaaatgc tgcatgtctt taaagaccgc cactggatca actgtggtca ggctggtccg
1261 ttaggctgga cgattccggc tgcgctaggg gtttgtgccg ctgatccgaa acgcaatgtg gtggcgattt
1331 ctggcgactt tgacttccag ttcctgattg aagagttagc tgttggcgcg cagttcaaca ttccgtacat
1401 ccatgtgctg gtcaacaacg cttatctggg gctgattcgt cagtcacaac gcgcttttga catggactac
1471 tgcgtgcaac tcgctttcga gaatatcaac tccagtgaag tgaatggcta cggtgttgac cacgtaaaag
1541 tagcggaagg tttaggttgt aaagctattc gggtcttcaa accggaagat attgcgccag cctttgaaca
1611 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttctcgagcg tgtgaccaat
1681 atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc cgataacgca gcggacgcac
1751 cgactgaaac ctgcttcatg cactatgaat aaGGGAGATA AATAATGTTA CGTTTCTCTG CTAATTTATC
1821 GATGTTATTT GGAGAATATG ATTTTCTCGC CCGTTTTGAG AAAGCTGCGC AGTGTGGTTT TC
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