MG1655
W3110
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Gene Report : pyrB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR19 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  pyrB
Related strains
     Strains Search Search strains by gene name:  pyrB   Search strains by all related name:  pyrB b4245 ECK4240 f311 JW4204

General information  (Go to Linear View:)
 Gene Name pyrB  
 Alternative name b4245,ECK4240,f311,JW4204  
 Location, Length 4,469,483 - 4,470,418 (  -  ) ;   96.33 min ; 936 (bp) ,   311 (aa) Go to Linear View
 Product aspartate carbamoyltransferase, catalytic subunit  
 Operon Name pyrLBI  
 Note GO_component: GO:0005737 - cytoplasm  
 Function enzyme; Pyrimidine ribonucleotide biosynthesis  
 Gene Ontology GO:0004070; aspartate carbamoyltransferase activity ( pyrB )
GO:0005737; cytoplasm ( pyrB )
GO:0006207; 'de novo' pyrimidine base biosynthetic process ( pyrB )
GO:0006221; pyrimidine nucleotide biosynthetic process ( pyrB )
GO:0006520; amino acid metabolic process ( pyrB )
GO:0016597; amino acid binding ( pyrB )
GO:0016740; transferase activity ( pyrB )
GO:0016743; carboxyl- or carbamoyltransferase activity ( pyrB )
 PID 2367364  
 EC number
  (KEGG Pathway)
 
2.1.3.2  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene pyrB ( EG10805 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) PYRB_ECOLI ( P00479 ; Q47555; Q47557 )
    -  Protein name Aspartate carbamoyltransferase catalytic chain  
    -  Synonyms EC 2.1.3.2, Aspartate transcarbamylase, ATCase  
    -  Gene name OrderedLocusNames=b4245, c5345, z5856, ECs5222, SF4245, S4507;  

 Linear View (Whole Mode)
View Location
   4460.0  –  4485.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP pyrB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, The Isolated, Unregulated Catalytic Trimer Of Aspartate Transcarbamoylase Complexed With Bisubstrate Analog Pala (N (Phosphonacetyl)-L-Aspartate)  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Aspartate carbamoyltransferase catalytic chain (Aspartate transcarbamylase) (ATCase)  
         PSI-BLAST Aspartate carbamoyltransferase catalytic chain (Aspartate transcarbamylase) (ATCase)
    nr     (database updated : 2007.02.20 )
         BLAST aspartate carbamoyltransferase catalytic subunit [Escherichia coli O157:H7 str. Sakai]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    CARBAMOYLTRANSFERASE     

Other Cross-Reference
    COG COG0540F 
    EcoCyc pyrB 

MMBR References
Proc Natl Acad Sci U S A. 1983;80:2462-2466
Nucleotide sequence of the structural gene (pyrB) that encodes the catalytic polypeptide of aspartate transcarbamoylase of Escherichia coli.
Hoover, T. A., W. D. Roof, K. F. Folterman, G. A. O'Donovan, D. A. Bencini, and J. R. Wild.
Nucleic Acids Res. 1988;16:2601-2612
Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.
Knott, V., D. J. G. Rees, Z. Cheng, and G. G. Brownlee.
Proc Natl Acad Sci U S A. 1985;82:4643-4647
Regulation of aspartate transcarbamoylase synthesis in Escherichia coli: analysis of deletion mutations in the promoter region of the pyrBI operon.
Levin, H. L., and H. K. Schachman.
Proc Natl Acad Sci U S A. 1983;80:1207-1211
Synthesis of aspartate transcarbamoylase in Escherichia coli: transcriptional regulation of the pyrB-pyrI operon.
Navre, M., and H. K. Schachman.
Proc Natl Acad Sci U S A. 1982;79:4020-4024
Genes encoding Escherichia coli aspartate transcarbomoylase: the pyrB-pyrI operon.
Pauza, C. D., M. J. Karels, M. Navre, and H. K. Schachman.
J Bacteriol. 1985;163(3):991-9.
Role of translation and attenuation in the control of pyrBI operon expression in Escherichia coli K-12.
Roland KL, Powell FE, Turnbough CL Jr. ( 3928602 )
Mol Gen Genet. 1982;187(3):391-400.
The organization and regulation of the pyrBI operon in E. coli includes a rho-independent attenuator sequence.
Roof WD, Foltermann KF, Wild JR. ( 6294470 )
Proc Natl Acad Sci U S A. 1983;80:368-372
Attenuation control of pyrBI operon expression in Escherichia coli K-12.
Turnbough, C. L., K. L. Hicks, and J. P. Donahue.

Sequences
Amino acid
FASTA format
0001 MANPLYQKHI ISINDLSRDD LNLVLATAAK LKANPQPELL KHKVIASCFF EASTRTRLSF ETSMHRLGAS 
0071 VVGFSDSANT SLGKKGETLA DTISVISTYV DAIVMRHPQE GAARLATEFS GNVPVLNAGD GSNQHPTQTL 
0141 LDLFTIQETQ GRLDNLHVAM VGDLKYGRTV HSLTQALAKF DGNRFYFIAP DALAMPQYIL DMLDEKGIAW 
0211 SLHSSIEEVM AEVDILYMTR VQKERLDPSE YANVKAQFVL RASDLHNAKA NMKVLHPLPR VDEIATDVDK 
0281 TPHAWYFQQA GNGIFARQAL LALVLNRDLV L

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gaaaaaagac gctggcctgc cgtttttctt 
-070 cccgttgatc acccattccc agcccctcaa tcgaggggct tttttttgcc caggcgtcag gagataaaag 
0001 atggctaatc cgctatatca gaaacatatc atttccataa acgaccttag tcgcgatgac cttaatctgg 
0071 tgctggcgac agcggcgaaa ctgaaagcaa acccgcaacc agagctgttg aagcacaaag tcattgccag 
0141 ctgtttcttc gaagcctcta cccgtacccg cctctctttc gaaacatcta tgcaccgcct gggggccagc 
0211 gtggtgggct tctccgacag cgccaataca tcactgggta aaaagggcga aacgctggcc gataccattt 
0281 cggttatcag cacttacgtc gatgcgatag tgatgcgtca tccgcaggaa ggtgcggcgc gcctggccac 
0351 cgagttttcc ggcaatgtac cggtactgaa tgccggtgat ggctccaacc aacatccgac gcaaaccttg 
0421 ctggacttat tcactattca ggaaacccag gggcgtctgg acaatctcca cgtcgcaatg gttggtgacc 
0491 tgaaatatgg ccgcaccgtt cactccctga ctcaggcgtt agcgaagttc gacggcaacc gtttttactt 
0561 catcgcgccg gacgcgctgg caatgccgca atacattctg gatatgctcg atgaaaaagg gatcgcatgg 
0631 agtctgcaca gctctattga agaagtgatg gcggaagtag acatcctgta catgacccgc gtgcaaaaag 
0701 agcgtctgga cccgtccgag tacgccaacg tgaaagcgca gtttgttctt cgcgccagcg atctccacaa 
0771 cgccaaagcc aatatgaaag tgctgcatcc gctgccgcgt gttgatgaga ttgcgacgga tgttgataaa 
0841 acgccacacg cctggtactt ccagcaggca ggcaacggga ttttcgctcg ccaggcgtta ctggcactgg 
0911 ttctgaatcg cgatctggta ctgtaagggg aaatagagat gacacacgat aataaattgc aggttgaagc 
0981 tattaaacgc ggcacggtaa ttgaccatat ccccgcccag atcggtttta agctgt