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PEC Original Annotations
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Essentiality
|
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Class
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non-essential
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|
References (PMID)
|
J Bacteriol. 1984;157(2):413-9.
Physical organization of the metJB component of the Escherichia coli K-12 metJBLF gene cluster.
Liljestrand-Golden CA, Johnson JR.
(
6319357
)
|
|
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Deletion
|
OCR09
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
metB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
metB
Search strains by all related name:
metB b3939 ECK3931 JW3910 met-1 o386
|
General information (Go to Linear View: )
|
|
Gene Name
|
metB
|
|
Alternative name
|
b3939,ECK3931,JW3910,met-1,o386
|
|
Location, Length
|
4,126,695 - 4,127,855
(
+
)
;
88.94
min
;
1161
(bp) ,
386
(aa)
|
|
Product
|
cystathionine gamma-synthase, PLP-dependent
|
|
Operon Name
|
metBL
|
|
Note
|
cystathionine gamma-synthase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009063 - cellular amino acid catabolic process; GO_process: GO:0009086 - methionine biosynthetic process
|
|
Function
|
enzyme; Amino acid biosynthesis: Methionine
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( metB )
GO:0003962
;
cystathionine gamma-synthase activity ( metB )
GO:0005515
;
protein binding ( metB )
GO:0005737
;
cytoplasm ( metB )
GO:0006520
;
amino acid metabolic process ( metB )
GO:0008652
;
amino acid biosynthetic process ( metB )
GO:0009086
;
methionine biosynthetic process ( metB )
GO:0016740
;
transferase activity ( metB )
GO:0030170
;
pyridoxal phosphate binding ( metB )
|
|
PID
|
1790375
|
EC number
(KEGG Pathway)
|
2.5.1.48
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
metB
(
EG10582
)
|
|
Number of removed
|
1 aa cleaved
|
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
METB_ECOLI
(
P00935
)
|
|
- Protein name
|
Cystathionine gamma-synthase
|
|
- Synonyms
|
EC 2.5.1.48, CGS, O-succinylhomoserine (Thiol)-lyase
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- Gene name
|
Name=metB; OrderedLocusNames=b3939;
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
metB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Methionine Gamma-Lyase (Mgl) From Trichomonas Vaginalis In Complex With Propargylglycine
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
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BLAST
|
Cystathionine gamma-synthase
|
|
PSI-BLAST
|
Serine hydroxymethyltransferase
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
cystathionine gamma-synthase [Escherichia coli K12]
|
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Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
|
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PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
CYS_MET_METAB_PP
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MMBR References
|
J Biol Chem. 1983;258:14868-14871
Structure of the metJBLF cluster in Escherichia coli K12. Sequence of the metB structural gene of the 5'- and 3'-flanking regions of the metBL operon.
Duchange, N., M. M. Zakin, P. Ferrara, I. Saint-Girons, I. Park, S. V. Tran, M.-C. Py, and G. N. Cohen.
|
J Bacteriol. 1984;159(2):767-9.
Insertion mutagenesis of the metJBLF gene cluster of Escherichia coli K-12: evidence for an metBL operon.
Greene RC, Smith AA.
(
6086586
)
|
Mol Gen Genet. 1982;187(3):401-4.
Localization of the metJBLF gene cluster of Escherichia coli in lambda met transducing phage.
Greene RC, Krueger JH, Johnson JR.
(
6294471
)
|
J Bacteriol. 1986;165(3):671-7.
Regulation of in vivo transcription of the Escherichia coli K-12 metJBLF gene cluster.
Kirby TW, Hindenach BR, Greene RC.
(
2419307
)
|
J Bacteriol. 1981;147(2):612-21.
Structural studies of lambda transducing bacteriophage carrying bacterial deoxyribonucleic acid from the metBJLF region of the Escherichia coli chromosome.
Krueger JH, Johnson JR, Greene RC, Dresser M.
(
6267016
)
|
J Bacteriol. 1984;157(2):413-9.
Physical organization of the metJB component of the Escherichia coli K-12 metJBLF gene cluster.
Liljestrand-Golden CA, Johnson JR.
(
6319357
)
|
J Biol Chem. 1984;259:14282-14285
Structure and autoregulation of the metJ regulatory gene in Escherichia coli.
Saint-Girons, I., N. Duchange, G. N. Cohen, and M. M. Zakin.
|
Mol Gen Genet. 1982;187(1):101-6.
Construction and physical mapping of plasmids containing the metJBLF gene cluster of E. coli K12.
Zakin MM, Greene RC, Dautry-Varsat A, Duchange N, Ferrara P, Py MC, Margarita D, Cohen GN.
(
6219268
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MTRKQATIAV RSGLNDDEQY GCVVPPIHLS STYNFTGFNE PRAHDYSRRG NPTRDVVQRA LAELEGGAGA
0071 VLTNTGMSAI HLVTTVFLKP GDLLVAPHDC YGGSYRLFDS LAKRGCYRVL FVDQGDEQAL RAALAEKPKL
0141 VLVESPSNPL LRVVDIAKIC HLAREVGAVS VVDNTFLSPA LQNPLALGAD LVLHSCTKYL NGHSDVVAGV
0211 VIAKDPDVVT ELAWWANNIG VTGGAFDSYL LLRGLRTLVP RMELAQRNAQ AIVKYLQTQP LVKKLYHPSL
0281 PENQGHEIAA RQQKGFGAML SFELDGDEQT LRRFLGGLSL FTLAESLGGV ESLISHAATM THAGMAPEAR
0351 AAAGISETLL RISTGIEDGE DLIADLENGF RAANKG
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CGCAAAGAAG TTTAGATGTC CAGATGTATT
-070 GACGTCCATT AACACAATGT TTACTCTGGT GCCTGACATT TCACCGACAA AGCCCAGGGA ACTTCATCAC
0001 atgacgcgta aacaggccac catcgcagtg cgtagcgggt taaatgacga cgaacagtat ggttgcgttg
0071 tcccaccgat ccatctttcc agcacctata actttaccgg atttaatgaa ccgcgcgcgc atgattactc
0141 gcgtcgcggc aacccaacgc gcgatgtggt tcagcgtgcg ctggcagaac tggaaggtgg tgctggtgca
0211 gtacttacta ataccggcat gtccgcgatt cacctggtaa cgaccgtctt tttgaaacct ggcgatctgc
0281 tggttgcgcc gcacgactgc tacggcggta gctatcgcct gttcgacagt ctggcgaaac gcggttgcta
0351 tcgcgtgttg tttgttgatc aaggcgatga acaggcatta cgggcagcgc tggcagaaaa acccaaactg
0421 gtactggtag aaagcccaag taatccattg ttacgcgtcg tggatattgc gaaaatctgc catctggcaa
0491 gggaagtcgg ggcggtgagc gtggtggata acaccttctt aagcccggca ttacaaaatc cgctggcatt
0561 aggtgccgat ctggtgttgc attcatgcac gaaatatctg aacggtcact cagacgtagt ggccggcgtg
0631 gtgattgcta aagacccgga cgttgtcact gaactggcct ggtgggcaaa caatattggc gtgacgggcg
0701 gcgcgtttga cagctatctg ctgctacgtg ggttgcgaac gctggtgccg cgtatggagc tggcgcagcg
0771 caacgcgcag gcgattgtga aatacctgca aacccagccg ttggtgaaaa aactgtatca cccgtcgttg
0841 ccggaaaatc aggggcatga aattgccgcg cgccagcaaa aaggctttgg cgcaatgttg agttttgaac
0911 tggatggcga tgagcagacg ctgcgtcgtt tcctgggcgg gctgtcgttg tttacgctgg cggaatcatt
0981 agggggagtg gaaagtttaa tctctcacgc cgcaaccatg acacatgcag gcatggcacc agaagcgcgt
1051 gctgccgccg ggatctccga gacgctgctg cgtatctcca ccggtattga agatggcgaa gatttaattg
1121 ccgacctgga aaatggcttc cgggctgcaa acaaggggta aAAATGAGTG TGATTGCGCA GGCAGGGGCG
1191 AAAGGTCGTC AGCTGCATAA ATTTGGTGGC AGTAGTCTGG CTGATGTGAA GTGTTATTTG CGTGTCGCGG
1261 G
|
