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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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OCL02
(D)
,
DE(OC3)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
pstB
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Related strains
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Strains Search
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Search strains by gene name:
pstB
Search strains by all related name:
pstB b3725 ECK3718 f257 JW3703 phoT
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General information (Go to Linear View: )
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Gene Name
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pstB
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Alternative name
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b3725,ECK3718,f257,JW3703,phoT
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Location, Length
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3,905,616 - 3,906,389
(
-
)
;
84.18
min
;
774
(bp) ,
257
(aa)
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Product
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phosphate transporter subunit
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Operon Name
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pstSCAB-phoU
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Note
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ATP-binding component of high-affinity phosphate-specific transport system; ATP-binding component of ABC superfamily; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006793 - phosphorus metabolic process
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Function
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transport; Transport of small molecules: Anions
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Gene Ontology
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GO:0000166
;
nucleotide binding ( pstB )
GO:0005315
;
inorganic phosphate transmembrane transporter activity ( pstB )
GO:0005524
;
ATP binding ( pstB )
GO:0005886
;
plasma membrane ( pstB )
GO:0006810
;
transport ( pstB )
GO:0006817
;
phosphate transport ( pstB )
GO:0015114
;
phosphate transmembrane transporter activity ( pstB )
GO:0015415
;
phosphate transmembrane-transporting ATPase activity ( pstB )
GO:0016020
;
membrane ( pstB )
GO:0016787
;
hydrolase activity ( pstB )
GO:0016887
;
ATPase activity ( pstB )
GO:0017111
;
nucleoside-triphosphatase activity ( pstB )
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PID
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1790162
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EC number
(KEGG Pathway)
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3.6.3.27
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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PSTA_ECOLI
(
P07654
)
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- Protein name
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Phosphate transport system permease protein pstA.
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- Synonyms
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- Gene name
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Name=pstA; Synonyms=phoT; OrderedLocusNames=b3726;
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MMBR References
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Mol Gen Genet. 1985;200(1):118-22.
The effect of the locus pstB on phosphate binding in the phosphate specific transport (PST) system of Escherichia coli.
Levitz R, Friedberg I, Brucker R, Fux A, Yagil E.
(
3897789
)
|
In B. P. Rosen, and S. Silver (ed.), Ion transport in prokaryotes. Academic Press, Inc., New York, N.Y. 1987:205-246
Phosphate transport in prokaryotes.
Rosenberg, H.
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J Bacteriol. 1993;175:6797-6809
Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon.
Steed, P. M., and B. L. Wanner.
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J Bacteriol. 1985;161(1):189-98.
Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships.
Surin BP, Rosenberg H, Cox GB.
(
3881386
)
|
J Bacteriol. 1996;178:3974-3977
PstB protein of the phosphate-specific transport system of Escherichia coli is an ATPase.
Chan, F. Y., and A. Torriani.
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J Bacteriol. 1982;152(2):692-701.
Cloning of and complementation tests with alkaline phosphatase regulatory genes (phoS and phoT) of Escherichia coli.
Amemura M, Shinagawa H, Makino K, Otsuji N, Nakata A.
(
6290447
)
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J Mol Biol. 1985;184(2):241-50.
Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli.
Amemura M, Makino K, Shinagawa H, Kobayashi A, Nakata A.
(
2993631
)
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| Sequences |
Amino acid
FASTA format
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0001 MSMVETAPSK IQVRNLNFYY GKFHALKNIN LDIAKNQVTA FIGPSGCGKS TLLRTFNKMF ELYPEQRAEG
0071 EILLDGDNIL TNSQDIALLR AKVGMVFQKP TPFPMSIYDN IAFGVRLFEK LSRADMDERV QWALTKAALW
0141 NETKDKLHQS GYSLSGGQQQ RLCIARGIAI RPEVLLLDEP CSALDPISTG RIEELITELK QDYTVVIVTH
0211 NMQQAARCSD HTAFMYLGEL IEFSNTDDLF TKPAKKQTED YITGRYG
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 acacggttaa cactttgccg gatgcggcgt
-070 gaacgcctga tccggcctac ggtaagcctg attagcgaag tgcatcaggc acgatgagga aaagattgca
0001 atgagtatgg ttgaaactgc cccgagtaaa attcaggttc gtaatttgaa cttctactac ggcaaattcc
0071 atgccctgaa aaacatcaac ctggatatcg ctaaaaacca ggtaacggcg tttatcgggc cgtccggctg
0141 cggtaaatcg acgctgctgc gtaccttcaa caaaatgttt gaactgtacc cggagcagcg tgcggaaggt
0211 gaaattctgc ttgatggcga caacatcctg accaactctc aggatatcgc actgctgcgt gcgaaagtgg
0281 gcatggtgtt ccagaaaccg acgccgtttc cgatgtccat ctacgacaac atcgcttttg gcgttcgtct
0351 gtttgagaag ctctcccgtg ccgacatgga cgagcgcgtg cagtgggcat tgaccaaagc cgcattgtgg
0421 aacgaaacca aagataaatt gcaccagagc ggttactctc tctctggtgg tcagcaacag cgtctgtgta
0491 ttgcgcgtgg tatcgccatt cgcccggaag tgctgctgct cgacgaaccg tgttcggcgc tcgaccctat
0561 ctctaccggg cgtattgaag agctgatcac cgaactgaag caggattaca ccgtggtgat cgtcacccac
0631 aacatgcagc aggctgcgcg ttgttccgac cacacggcgt ttatgtacct gggcgaattg attgagttca
0701 gcaacacgga cgatctgttc accaagccag cgaagaaaca aacagaagac tacatcaccg gtcgttacgg
0771 ttgattcagg agtgcgttat ggacagtctc aatcttaata aacatatttc cggccagttc aacgccgaac
0841 tggaaagtat ccgcacgcag gtgatgacca tggg
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