|
PEC Original Annotations
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|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCL17
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
nikE
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
nikE
Search strains by all related name:
nikE b3480 ECK3464 hydC hydD JW3445 o268
|
General information (Go to Linear View: )
|
|
Gene Name
|
nikE
|
|
Alternative name
|
b3480,ECK3464,hydC,hydD,JW3445,o268
|
|
Location, Length
|
3,615,799 - 3,616,605
(
+
)
;
77.93
min
;
807
(bp) ,
268
(aa)
|
|
Product
|
nickel transporter subunit
|
|
Operon Name
|
nikABCDER
|
|
Note
|
ATP-binding protein of nickel transport system; ATP-binding component of ABC superfamily; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009060 - aerobic respiration; GO_process: GO:0009061 - anaerobic respiration
|
|
Function
|
transport; Transport of small molecules: Cations
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( nikE )
GO:0005524
;
ATP binding ( nikE )
GO:0005886
;
plasma membrane ( nikE )
GO:0006810
;
transport ( nikE )
GO:0015413
;
nickel-transporting ATPase activity ( nikE )
GO:0015675
;
nickel ion transport ( nikE )
GO:0016020
;
membrane ( nikE )
GO:0016151
;
nickel ion binding ( nikE )
GO:0016787
;
hydrolase activity ( nikE )
GO:0016887
;
ATPase activity ( nikE )
GO:0017111
;
nucleoside-triphosphatase activity ( nikE )
|
|
PID
|
1789891
|
EC number
(KEGG Pathway)
|
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
NIKE_ECOLI
(
P33594
)
|
|
- Protein name
|
Nickel transport ATP-binding protein nikE.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=nikE; OrderedLocusNames=b3480;
|
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Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
nikE
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain 1, Malk
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Nickel import ATP-binding protein nikE
|
|
PSI-BLAST
|
Phosphate import ATP-binding protein pstB
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
nickel transporter subunit [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
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PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
ATP_GTP_A
ABC_TRANSPORTER
|
|
MMBR References
|
Mol Microbiol. 1993;9(6):1181-91.
The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel.
Navarro C, Wu LF, Mandrand-Berthelot MA.
(
7934931
)
|
Gene. 1991;107(1):37-42.
The hydC region contains a multi-cistronic operon (nik) involved in nickel transport in Escherichia coli.
Wu LF, Navarro C, Mandrand-Berthelot MA.
(
1743519
)
|
Biochimie. 1986;68(1):167-79.
Genetic and physiological characterization of new Escherichia coli mutants impaired in hydrogenase activity.
Wu LF, Mandrand-Berthelot MA.
(
3089308
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MTLLNISGLS HHYAHGGFNG KHQHQAVLNN VSLTLKSGET VALLGRSGCG KSTLARLLVG LESPAQGNIS
0071 WRGEPLAKLN RAQRKAFRRD IQMVFQDSIS AVNPRKTVRE ILREPMRHLL SLKKSEQLAR ASEMLKAVDL
0141 DDSVLDKRPP QLSGGQLQRV CLARALAVEP KLLILDEAVS NLDLVLQAGV IRLLKKLQQQ FGTACLFITH
0211 DLRLVERFCQ RVMVMDNGQI VETQVVGEKL TFSSDAGRVL QNAVLPAFPV RRRTTEKV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AACAGGGCGA TGTAGAAACG CTGTTTAACG
-070 CCCCCAAACA TACAGTGACG CGCAGCCTGG TTTCCGCTCA TCTCGCCCTC TACGGTATGG AGCTGGCATC
0001 atgactttac ttaacatctc cggcctttcc catcactatg cgcacggtgg atttaacgga aaacatcaac
0071 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc tggggcgcag
0141 cggctgcggg aaaagtaccc tcgcgcggtt gctggtgggt ttagaatcgc ccgcgcaggg gaatattagc
0211 tggcgtggcg aaccgctggc gaaactcaat cgcgcccagc gtaaagcgtt ccgccgcgat attcagatgg
0281 tatttcagga ctccatcagc gccgtgaatc cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg
0351 ccacctgcta tcactgaaaa aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc
0421 gatgacagcg ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagcgcgtc tgcctggctc
0491 gcgcgctggc ggtcgaaccg aaactactga ttctggatga agccgtttct aaccttgatc tcgtgttaca
0561 ggcgggtgtc attcgcctgc tgaaaaagct acaacaacag tttggcaccg cctgcctgtt catcacccac
0631 gacttacgcc tggtagaacg cttttgccag cgggtaatgg ttatggacaa cggacaaatc gtcgaaaccc
0701 aggtggtggg agagaaatta accttttcct ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc
0771 attccccgtg cgccgtcgca ccacagaaaa ggtttaaCGC AAATGCAACG AGTCACCATC ACGCTTGATG
0841 ACGATTTACT GGAGACGCTG GACAGCCTGA GCCAGCGTCG TGGTTATAAC AACCGTTCCG AAGCTAT
|
