|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCL54
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
purL
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
purL
Search strains by all related name:
purL b2557 ECK2555 JW2541 purG purI
|
General information (Go to Linear View: )
|
|
Gene Name
|
purL
|
|
Alternative name
|
b2557,ECK2555,JW2541,purG,purI
|
|
Location, Length
|
2,689,678 - 2,693,565
(
-
)
;
57.97
min
;
3888
(bp) ,
1295
(aa)
|
|
Product
|
phosphoribosylformyl-glycineamide synthetase
|
|
Operon Name
|
purL
|
|
Note
|
phosphoribosylformyl-glycineamide synthetase = FGAM synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006164 - purine nucleotide biosynthetic process
|
|
Function
|
enzyme; Purine ribonucleotide biosynthesis
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( purL )
GO:0003824
;
catalytic activity ( purL )
GO:0004642
;
phosphoribosylformylglycinamidine synthase activity ( purL )
GO:0005524
;
ATP binding ( purL )
GO:0005737
;
cytoplasm ( purL )
GO:0006164
;
purine nucleotide biosynthetic process ( purL )
GO:0006189
;
'de novo' IMP biosynthetic process ( purL )
GO:0006541
;
glutamine metabolic process ( purL )
GO:0016874
;
ligase activity ( purL )
|
|
PID
|
48994899
|
EC number
(KEGG Pathway)
|
6.3.5.3
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
purL
(
EG10797
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
PUR4_ECOLI
(
P15254
;
P78097
)
|
|
- Protein name
|
Phosphoribosylformylglycinamidine synthase
|
|
- Synonyms
|
EC 6.3.5.3, FGAM synthase, FGAMS, Formylglycinamide ribotide amidotransferase, FGARAT, Formylglycinamide ribotide synthetase
|
|
- Gene name
|
Name=purL; Synonyms=purI; OrderedLocusNames=b2557;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
purL
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Structure Of Formylglycinamide Synthetase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Phosphoribosylformylglycinamidine synthase
|
|
PSI-BLAST
|
Phosphoribosylformylglycinamidine synthase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
phosphoribosylformylglycinamidine synthase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
J Bacteriol. 1983;154(3):1485-8.
Identification of the enzymatic reactions encoded by the purG and purI genes of Escherichia coli.
Houlberg U, Hove-Jensen B, Jochimsen B, Nygaard P.
(
6343356
)
|
J Biol Chem. 1989;264:21230-21238
The organization of the purL gene encoding 5'-phosphoribosylformylglycinamide amidotransferase of Escherichia coli.
Sampei, G., and K. Mizobuchi.
|
Biochemistry. 1989;28(6):2459-71.
Formylglycinamide ribonucleotide synthetase from Escherichia coli: cloning, sequencing, overproduction, isolation, and characterization.
Schendel FJ, Mueller E, Stubbe J, Shiau A, Smith JM.
(
2659070
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MMEILRGSPA LSAFRINKLL ARFQAARLPV HNIYAEYVHF ADLNAPLNDD EHAQLERLLK YGPALASHAP
0071 QGKLLLVTPR PGTISPWSSK ATDIAHNCGL QQVNRLERGV AYYIEAGTLT NEQWQQVTAE LHDRMMETVF
0141 FALDDAEQLF AHHQPTPVTS VDLLGQGRQA LIDANLRLGL ALAEDEIDYL QDAFTKLGRN PNDIELYMFA
0211 QANSEHCRHK IFNADWVIDG EQQPKSLFKM IKNTFETTPD HVLSAYKDNA AVMEGSEVGR YFADHETGRY
0281 DFHQEPAHIL MKVETHNHPT AISPWPGAAT GSGGEIRDEG ATGRGAKPKA GLVGFSVSNL RIPGFEQPWE
0351 EDFGKPERIV TALDIMTEGP LGGAAFNNEF GRPALNGYFR TYEEKVNSHN GEELRGYHKP IMLAGGIGNI
0421 RADHVQKGEI NVGAKLVVLG GPAMNIGLGG GAASSMASGQ SDADLDFASV QRDNPEMERR CQEVIDRCWQ
0491 LGDANPILFI HDVGAGGLSN AMPELVSDGG RGGKFELREI LSDEPGMSPL EIWCNESQER YVLAVAADQL
0561 PLFDELCKRE RAPYAVIGEA TEELHLSLHD RHFDNQPIDL PLDVLLGKTP KMTRDVQTLK AKGDALAREG
0631 ITIADAVKRV LHLPTVAEKT FLVTIGDRSV TGMVARDQMV GPWQVPVANC AVTTASLDSY YGEAMAIGER
0701 APVALLDFAA SARLAVGEAL TNIAATQIGD IKRIKLSANW MAAAGHPGED AGLYEAVKAV GEELCPALGL
0771 TIPVGKDSMS MKTRWQEGNE EREMTSPLSL VISAFARVED VRHTITPQLS TEDNALLLID LGKGNNALGA
0841 TALAQVYRQL GDKPADVRDV AQLKGFYDAI QALVAQRKLL AYHDRSDGGL LVTLAEMAFA GHCGIDADIA
0911 TLGDDRLAAL FNEELGAVIQ VRAADREAVE SVLAQHGLAD CVHYVGQAVS GDRFVITANG QTVFSESRTT
0981 LRVWWAETTW QMQRLRDNPE CADQEHQAKS NDADPGLNVK LSFDINEDVA APYIATGARP KVAVLREQGV
1051 NSHVEMAAAF HRAGFDAIDV HMSDLLTGRT GLEDFHALVA CGGFSYGDVL GAGEGWAKSI LFNDRVRDEF
1121 ATFFHRPQTL ALGVCNGCQM MSNLRELIPG SELWPRFVRN TSDRFEARFS LVEVTQSPSL LLQGMVGSQM
1191 PIAVSHGEGR VEVRDAAHLA ALESKGLVAL RYVDNFGKVT ETYPANPNGS PNGITAVTTE SGRVTIMMPH
1261 PERVFRTVSN SWHPENWGED GPWMRIFRNA RKQLG
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttatttccac gcaaacggtt tcgtcagcgc
-070 atcagattct ttataatgac gcccgtttcc cccccttggg tacaccgaaa gcttagaaga cgagagactt
0001 atgatggaaa ttctgcgtgg ttcgcctgca ctgtcggcat tccgaatcaa caaactgctg gcacgttttc
0071 aggctgccag gctcccggtt cacaatattt acgccgagta tgtccatttt gctgacctca atgcgccgtt
0141 aaacgatgat gagcacgcac aacttgaacg cctgctgaaa tatggcccgg cactcgccag ccacgccccg
0211 caaggcaaac tcctgctggt gaccccgcgt cctggcacca tctctccctg gtcttcgaaa gcgaccgata
0281 ttgcccataa ctgcgggcta caacaggtaa accgccttga gcgcggcgtt gcttactata tagaagccgg
0351 tacgctgacc aatgaacaat ggcagcaggt taccgctgaa ctgcacgacc gcatgatgga aacggtcttt
0421 tttgctttag atgatgcaga gcagttgttt gcccaccatc aaccgactcc ggttaccagc gttgatttgc
0491 tggggcaggg ccgtcaggcg ctgatcgacg ctaacctgcg tcttggcttg gctctggcgg aagatgaaat
0561 tgactatctg caggatgctt tcacaaagct tggtcgtaac ccgaacgaca tcgaactgta tatgtttgcc
0631 caggcgaact ccgagcactg ccgccacaaa atttttaacg ccgactgggt tatcgatggt gaacagcagc
0701 cgaaatcgct gttcaagatg atcaaaaata ctttcgaaac cacgccagat cacgttctct ctgcttataa
0771 agataacgcc gccgtaatgg aaggttctga agtgggccgc tactttgctg accacgaaac gggccgctac
0841 gatttccatc aggaaccggc gcatattctg atgaaagtcg aaactcacaa ccacccgacg gcgatttctc
0911 cgtggccggg cgcggcgacc ggttccggcg gtgaaatccg cgatgaaggt gccaccgggc gcggcgcaaa
0981 gccgaaagcg ggtctggttg gtttctccgt ttccaacctg cgaattcctg gcttcgaaca gccgtgggaa
1051 gaagatttcg gtaagcctga gcgcattgtc accgcgctgg acatcatgac cgaaggcccg ctgggcggcg
1121 cggcgtttaa caacgaattt ggtcgtccgg cactgaacgg ctacttccgt acttatgaag aaaaagtgaa
1191 cagccacaac ggcgaagagc tgcgcggtta tcacaaaccg atcatgctgg cgggcgggat cggcaacatt
1261 cgcgccgatc acgtacaaaa aggcgagatc aacgtcggtg cgaagctggt cgttctcggc ggcccggcaa
1331 tgaacatcgg tcttggcggt ggtgcagcgt cttctatggc gtctggtcag tctgatgccg acctcgactt
1401 tgcttccgta cagcgcgaca acccggagat ggagcgtcgc tgccaggaag tgatcgaccg ttgctggcag
1471 cttggtgatg ccaacccaat cctgtttatc cacgacgttg gcgctggcgg tctttctaac gccatgccgg
1541 aactggtgag cgacggcggg cgcggcggta aatttgaact gcgcgagatt ctaagcgacg aaccgggcat
1611 gagcccgctg gaaatctggt gtaacgaatc ccaggaacgc tacgtgctgg cggttgctgc cgatcaatta
1681 ccgctgtttg acgaactgtg taagcgtgag cgcgcaccct acgcggtgat tggtgaagcg accgaagaac
1751 tgcatctttc tctgcacgat cgtcattttg ataatcagcc gatcgatctg ccgctggacg tcctgcttgg
1821 taaaacgccg aagatgaccc gcgatgtaca aacgctgaaa gcgaaaggcg acgcgctggc ccgtgaaggg
1891 atcaccattg cagacgcggt gaaacgtgtg ctgcatctgc cgactgtggc ggaaaaaacc ttcctggtga
1961 ccattggcga ccgcagcgta accggcatgg tagcgcgcga tcagatggtg gggccgtggc aggtgccggt
2031 cgctaactgc gcggtcacta ccgccagcct cgacagctac tacggtgaag cgatggcgat tggcgagcgt
2101 gcgccggttg cgctgctgga tttcgccgcc tctgcccgtc tggcggtcgg tgaagcgtta accaacatcg
2171 ccgcaacaca aattggcgat atcaaacgca tcaaactttc cgccaactgg atggcggcgg caggccaccc
2241 tggtgaagat gcgggcctgt atgaagccgt taaagccgtg ggcgaagagc tttgtccggc gctgggcctg
2311 acgatcccgg tgggtaaaga ctccatgtcg atgaaaaccc gctggcagga aggtaacgaa gagcgcgaaa
2381 tgacgtcgcc gctgtcgctg gtgatttctg catttgcccg cgtggaagat gtacgtcaca ccatcacgcc
2451 gcagctttct accgaagata acgcactgct gctgattgat ttgggcaaag gcaataacgc gctgggcgca
2521 acggcgctgg cgcaggttta tcgtcagctt ggcgacaaac cggcagatgt acgcgatgtc gcgcaactga
2591 aaggcttcta tgacgcgatt caggcgctgg ttgcacagcg taagctgctg gcgtatcacg accgctctga
2661 tggcggcctg ctggtaacgc tggcggaaat ggcctttgct ggtcattgtg gcattgacgc ggatatcgcc
2731 actctgggtg acgatcgcct ggcggcgttg tttaacgaag aactgggtgc ggtgattcag gttcgtgccg
2801 ctgaccgtga agcggtcgag tccgtactgg cacagcatgg gcttgctgat tgtgtccatt atgtagggca
2871 ggcggtttcc ggtgaccgtt ttgtgattac cgccaacggg cagactgtat tcagcgaaag ccgcaccacg
2941 ttgcgtgtct ggtgggcaga aactacctgg cagatgcagc gcctgcgtga caacccggag tgtgccgatc
3011 aggagcatca ggcgaaatct aacgacgccg atccgggcct gaatgtaaaa ctgtcgttcg atatcaacga
3081 agatgtggca gcaccgtata ttgccactgg cgcacgtccg aaagttgccg tactgcgtga gcagggcgtg
3151 aactcgcatg ttgaaatggc ggcagctttc caccgtgcag gctttgatgc tatcgacgtg catatgagtg
3221 acctgctgac cggacgcacg ggcctggaag atttccacgc cctggtcgcg tgcggtggtt tctcctacgg
3291 tgatgtgctg ggtgccggtg aaggttgggc gaagtcaatc ctgttcaatg accgtgtacg cgatgagttt
3361 gcaaccttct tccaccgtcc gcaaacgctg gcgctggggg tatgtaacgg ttgccagatg atgtctaatc
3431 tgcgtgaact gatcccaggt agtgagttgt ggccacgttt tgtgcgcaat acctccgatc gctttgaagc
3501 gcgtttcagc ctggttgaag taacccaaag cccgtctctg ctgttgcagg ggatggtggg ctcgcaaatg
3571 ccgattgctg tctctcatgg tgaagggcgc gtggaagtgc gtgatgcggc gcatctggcg gcactggaaa
3641 gcaaagggct ggtggcactg cgctatgtcg ataacttcgg caaagtcact gaaacctacc cggctaaccc
3711 gaacggttca ccgaacggta ttacggcagt cacgactgaa agtggtcgag tcaccattat gatgccgcac
3781 ccggaacgtg ttttccgtac tgtcagcaac tcctggcatc cggaaaactg gggcgaggat ggcccatgga
3851 tgcgcatttt ccgcaatgcg cgtaagcagt tggggtaagt cgtcagccca ttggttttgc agcccggatg
3921 cggcattaag caccaaccac tatcataagc tcccaactcc gggagctttt ttgtgtctgt aaatcacg
|
