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PEC Original Annotations
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Essentiality
|
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Class
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essential
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References (PMID)
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Arch Biochem Biophys. 1991;284(1):9-16.
Mutagenesis of the folC gene encoding folylpolyglutamate synthetase-dihydrofolate synthetase in Escherichia coli.
Kimlova LJ, Pyne C, Keshavjee K, Huy J, Beebakhee G, Bognar AL.
(
1989505
)
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Deletion
|
OCL64
(D)
,
OCL64-9
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
folC
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Related strains
|
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Strains Search
|
Search strains by gene name:
folC
Search strains by all related name:
folC b2315 dedC ECK2309 JW2312
|
General information (Go to Linear View: )
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Gene Name
|
folC
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Alternative name
|
b2315,dedC,ECK2309,JW2312
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|
Location, Length
|
2,429,696 - 2,430,964
(
-
)
;
52.37
min
;
1269
(bp) ,
422
(aa)
|
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Product
|
bifunctional folylpolyglutamate synthase/ dihydrofolate synthase
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Operon Name
|
folC-dedD
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Note
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dihydrofolate:folylpolyglutamate synthetase; dihydrofolate synthetase; GO_process: GO:0046656 - folic acid biosynthetic process
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Function
|
enzyme; Biosynthesis of cofactors, carriers: Folic acid
|
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Gene Ontology
|
GO:0000166
;
nucleotide binding ( folC )
GO:0003824
;
catalytic activity ( folC )
GO:0004326
;
tetrahydrofolylpolyglutamate synthase activity ( folC )
GO:0005515
;
protein binding ( folC )
GO:0005524
;
ATP binding ( folC )
GO:0006730
;
one-carbon compound metabolic process ( folC )
GO:0008841
;
dihydrofolate synthase activity ( folC )
GO:0009058
;
biosynthetic process ( folC )
GO:0009396
;
folic acid and derivative biosynthetic process ( folC )
GO:0016874
;
ligase activity ( folC )
GO:0046656
;
folic acid biosynthetic process ( folC )
|
|
PID
|
1788654
|
EC number
(KEGG Pathway)
|
6.3.2.12
6.3.2.17
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
folC
(
EG10327
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
FOLC_ECOLI
(
P08192
;
P78237
)
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- Protein name
|
FolC bifunctional protein
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- Synonyms
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- Gene name
|
Name=folC; Synonyms=dedC; OrderedLocusNames=b2315;
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MMBR References
|
In J. Blair (ed.), Chemistry and biology of pteridines. W. de Gruyter, New York, N.Y. 1983:585-589
Isolation and characterization of an E. coli mutant affected in dihydrofolate- and folylpolyglutamate-synthetase.
Ferone, R., S. C. Singer, M. H. Hanlon, and S. Roland.
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J Biol Chem. 1987;262:12209-12217
The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons.
Nonet, M. L., C. C. Marvel, and D. R. Tolan.
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J Biol Chem. 1987;262:12337-12343
Primary structure of the Escherichia coli folC gene and its folypolyglutamate synthetase-dihydrofolate synthetase product and regulation of expression by an upstream gene.
Bognar, A. L., C. Osborne, and B. Shane.
|
| Sequences |
Amino acid
FASTA format
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0001 MIIKRTPQAA SPLASWLSYL ENLHSKTIDL GLERVSLVAA RLGVLKPAPF VFTVAGTNGK GTTCRTLESI
0071 LMAAGYKVGV YSSPHLVRYT ERVRVQGQEL PESAHTASFA EIESARGDIS LTYFEYGTLS ALWLFKQAQL
0141 DVVILEVGLG GRLDATNIVD ADVAVVTSIA LDHTDWLGPD RESIGREKAG IFRSEKPAIV GEPEMPSTIA
0211 DVAQEKGALL QRRGVEWNYS VTDHDWAFSD AHGTLENLPL PLVPQPNAAT ALAALRASGL EVSENAIRDG
0281 IASAILPGRF QIVSESPRVI FDVAHNPHAA EYLTGRMKAL PKNGRVLAVI GMLHDKDIAG TLAWLKSVVD
0351 DWYCAPLEGP RGATAEQLLE HLGNGKSFDS VAQAWDAAMA DAKAEDTVLV CGSFHTVAHV MEVIDARRSG
0421 GK
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 cccggtaccg gatcaggaac ctgaggcctg
-070 ataactgata agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc agcggatacc
0001 atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct ttcttatctg gaaaacctgc
0071 acagtaaaac tatcgatctc ggccttgagc gcgtgagcct ggtcgcggcg cgtcttggcg tcctgaaacc
0141 agcgccattt gtgtttaccg ttgcgggtac gaatggcaaa ggcaccacct gccgtacgct ggagtcgatt
0211 ctgatggcgg cagggtacaa agtgggcgtc tacagttcgc ctcatctggt gcgttatacc gagcgcgtac
0281 gtgtgcaggg ccaggaattg ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg
0351 tgatatttcc ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaagca ggcacaactt
0421 gacgtggtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa tattgtcgac gccgatgtcg
0491 cggtagtaac cagtattgcg ctggatcata ccgactggct gggtccagat cgcgaaagta ttggtcgcga
0561 gaaagcaggc atcttccgca gcgaaaaacc ggcaattgtc ggtgagccgg aaatgccttc taccattgct
0631 gatgtggcgc aggaaaaagg tgcactgtta caacgtcggg gcgttgagtg gaactattcc gtcaccgatc
0701 atgactgggc gtttagcgat gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa
0771 tgccgcaaca gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat tcgcgacggg
0841 attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc acgcgttatt tttgatgtcg
0911 cgcataatcc acatgcggcg gaatatctca ccgggcgtat gaaagcgcta ccgaaaaacg ggcgcgtgct
0981 ggcggttatc ggtatgctac atgataaaga tattgccgga actctggcct ggttgaaaag cgtggttgat
1051 gactggtatt gtgcgccact ggaagggccg cgcggtgcca cggcagaaca actgcttgag catttgggta
1121 acggcaaatc atttgatagc gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac
1191 cgtgctggtg tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag gagaagcggt
1261 ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc tgggggtgat tgtacttcca
1331 gggctgctgg acgggcagaa aaaacattat caggatgag
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