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PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Gene. 1996;168(1):43-8.
Menaquinone (vitamin K2) biosynthesis: localization and characterization of the menE gene from Escherichia coli.
Sharma V, Hudspeth ME, Meganathan R.
(
8626063
)
|
|
|
Deletion
|
13
(LD)
,
OCL67
(D)
,
OCL67,68-3 (LD 13B_CRS)
(D)
,
OCL67,68-4 (LD 13B_PO)
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
menD
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
menD
Search strains by all related name:
menD b2264 ECK2258 f556 JW5374
|
General information (Go to Linear View: )
|
|
Gene Name
|
menD
|
|
Alternative name
|
b2264,ECK2258,f556,JW5374
|
|
Location, Length
|
2,375,611 - 2,377,281
(
-
)
;
51.20
min
;
1671
(bp) ,
556
(aa)
|
|
Product
|
bifunctional 2-oxoglutarate decarboxylase/ SHCHC synthase
|
|
Operon Name
|
menFDHBCE
|
|
Note
|
2-oxoglutarate decarboxylase; SHCHC synthase; GO_process: GO:0009061 - anaerobic respiration
|
|
Function
|
enzyme; Biosynthesis of cofactors, carriers: Menaquinone, ubiquinone
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( menD )
GO:0008683
;
2-oxoglutarate decarboxylase activity ( menD )
GO:0009234
;
menaquinone biosynthetic process ( menD )
GO:0016740
;
transferase activity ( menD )
GO:0016829
;
lyase activity ( menD )
GO:0030976
;
thiamin pyrophosphate binding ( menD )
GO:0046410
;
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase activity ( menD )
|
|
PID
|
1788599
|
EC number
(KEGG Pathway)
|
2.2.1.9
4.1.1.71
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
MEND_ECOLI
(
P17109
;
P76478; P78180; P78181
)
|
|
- Protein name
|
Menaquinone biosynthesis protein menD
|
|
- Synonyms
|
|
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- Gene name
|
Name=menD; OrderedLocusNames=b2264;
|
|
MMBR References
|
J Gen Microbiol. 1979;115(2):259-71.
Anaerobic growth of Escherichia coli K12 with fumarate as terminal electron acceptor. Genetic studies with menaquinone and fluoroacetate-resistant mutants.
Guest JR.
(
393800
)
|
Mol Gen Genet. 1981;181(3):379-83.
Molecular cloning of menaquinone biosynthetic genes of Escherichia coli K12.
Guest JR, Shaw DJ.
(
6454056
)
|
J Bacteriol. 1992;174:8111-8118
Menaquinone (vitamin K2) biosynthesis: evidence that the Escherichia coli menD gene encodes both 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid synthase and alpha-ketoglutarate decarboxylase activities.
Palaniappan, C., V. Sharma, M. E. S. Hudspeth, and R. Meganathan.
|
J Bacteriol. 1989;171(8):4349-54.
Sequence and overexpression of the menD gene from Escherichia coli.
Popp JL.
(
2666397
)
|
FEMS Microbiol Lett. 1983;17:63-67.
Recombinant plasmids containing menaquinone biosynthetic genes of Escherichia coli.
Shaw, D. J., E. C. Robinson, R. Meganathan, R. Bentley, and J. R. Guest.
|
J Bacteriol. 1982;152(3):1132-7.
Characterization of Escherichia coli men mutants defective in conversion of o-succinylbenzoate to 1,4-dihydroxy-2-naphthoate.
Shaw DJ, Guest JR, Meganathan R, Bentley R.
(
6754698
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSVSAFNRRW AAVILEALTR HGVRHICIAP GSRSTPLTLA AAENSAFIHH THFDERGLGH LALGLAKVSK
0071 QPVAVIVTSG TAVANLYPAL IEAGLTGEKL ILLTADRPPE LIDCGANQAI RQPGMFASHP THSISLPRPT
0141 QDIPARWLVS TIDHALGTLH AGGVHINCPF AEPLYGEMDD TGLSWQQRLG DWWQDDKPWL REAPRLESEK
0211 QRDWFFWRQK RGVVVAGRMS AEEGKKVALW AQTLGWPLIG DVLSQTGQPL PCADLWLGNA KATSELQQAQ
0281 IVVQLGSSLT GKRLLQWQAS CEPEEYWIVD DIEGRLDPAH HRGRRLIANI ADWLELHPAE KRQPWCVEIP
0351 RLAEQAMQAV IARRDAFGEA QLAHRICDYL PEQGQLFVGN SLVVRLIDAL SQLPAGYPVY SNRGASGIDG
0421 LLSTAAGVQR ASGKPTLAIV GDLSALYDLN ALALLRQVSA PLVLIVVNNN GGQIFSLLPT PQSERERFYL
0491 MPQNVHFEHA AAMFELKYHR PQNWQELETA FADAWRTPTT TVIEMVVNDT DGAQTLQQLL AQVSHL
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 caaatggaat agtaatgagt cgcatcatta
-070 ccgattcata tcaataatct atttttgtag ctccttatac ttagccgcaa tattgatacc ggacaaactc
0001 atgtcagtaa gcgcatttaa ccgacgctgg gcggcggtca ttctggaagc attaacgcgt cacggcgtca
0071 gacacatctg tatcgcccca ggctcgcgtt ctacaccgtt aacgttagcg gcggcggaga attccgcatt
0141 cattcaccac acccatttcg atgagcgtgg gttggggcat ctggcgctgg ggctggcgaa agtcagcaag
0211 cagccggtgg cggtgattgt gacctccggc acggcggtgg caaatctcta tccggcactg attgaagccg
0281 ggttaaccgg agaaaaactg attctcttaa ccgccgatcg cccgccggag ctaattgact gcggcgcgaa
0351 tcaggcaatt cgccagccgg gaatgttcgc ctctcacccc acgcacagta tttcattgcc gcgcccgacc
0421 caggatatcc ccgcacgttg gctggtttct accatcgacc acgctctcgg tacgcttcat gcggggggag
0491 tccatatcaa ctgcccgttt gctgaaccgc tgtatggcga aatggacgat accgggctta gctggcaaca
0561 gcgtctgggt gactggtggc aggacgacaa accgtggctg cgtgaagcgc ctcgtctgga aagtgaaaaa
0631 cagcgcgact ggttcttctg gcgacaaaag cgcggcgtgg tggttgccgg gcgcatgagt gcggaagagg
0701 gcaaaaaagt tgccctgtgg gcgcaaactc ttggctggcc gctgattggc gatgtgctgt cacaaaccgg
0771 gcagccgctg ccgtgtgccg atctttggtt aggcaatgcc aaagcgacca gcgagctgca gcaggcgcaa
0841 attgtggtgc aactgggaag cagcctgacg gggaaacggc tcctgcaatg gcaggcaagc tgtgaaccag
0911 aagagtactg gattgttgat gacattgaag ggcgacttga tccggcacac catcgcggac gtcgcttaat
0981 tgccaatatt gccgactggc tggagctgca tccggcagaa aaacgccagc cctggtgcgt tgaaatcccg
1051 cgcctggcgg aacaggcaat gcaggcggtt attgcccgcc gtgatgcgtt tggcgaagcg caactggcgc
1121 atcgcatctg cgactatctg cctgaacagg ggcaattgtt tgttggtaac agcctggtgg tacgtctgat
1191 tgatgcgctt tcgcaacttc cggcaggtta cccggtgtac agcaaccgtg gggccagcgg tatcgacggg
1261 ctgctttcga ccgccgccgg cgttcagcgg gcaagcggca aaccgacgct ggcgattgtg ggcgatctct
1331 ccgcacttta cgatctcaac gcgctggcgt tattgcgtca ggtttctgcg ccgctggtat taattgtggt
1401 gaacaacaac ggcgggcaaa ttttctcgct gttgccaacg ccgcaaagcg agcgtgagcg tttctatctg
1471 atgccgcaaa acgtccattt tgagcacgcc gccgcgatgt tcgagctgaa atatcatcgt ccgcaaaact
1541 ggcaggaact tgaaacggca tttgccgacg cctggcgcac gccaaccacc acggtgattg aaatggtggt
1611 taacgacacc gatggtgcgc aaacgctcca gcaacttctg gcgcaggtaa gccatttatg atcctgcacg
1681 cgcaggcaaa acacggaaaa ccaggtttac cctggctggt gtttttgcac ggtttttccg gcgattgcca
1751 cgaatggcaa gaagtgggcg a
|
