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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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J Bacteriol. 1994;176(3):815-21.
holE, the gene coding for the theta subunit of DNA polymerase III of Escherichia coli: characterization of a holE mutant and comparison with a dnaQ (epsilon-subunit) mutant.
Slater SC, Lifsics MR, O'Donnell M, Maurer R.
(
8300534
)
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Deletion
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OCR36
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
dnaQ
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Related strains
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Strains Search
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Search strains by gene name:
dnaQ
Search strains by all related name:
dnaQ b0215 ECK0215 JW0205 mutD
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General information (Go to Linear View: )
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Gene Name
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dnaQ
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Alternative name
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b0215,ECK0215,JW0205,mutD
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Location, Length
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236,067 - 236,798
(
+
)
;
5.09
min
;
732
(bp) ,
243
(aa)
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Product
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DNA polymerase III epsilon subunit
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Operon Name
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dnaQ
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Note
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DNA polymerase III, epsilon subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication; GO_process: GO:0006457 - protein folding
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Function
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enzyme; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0000287
;
magnesium ion binding ( dnaQ )
GO:0003676
;
nucleic acid binding ( dnaQ )
GO:0003677
;
DNA binding ( dnaQ )
GO:0003887
;
DNA-directed DNA polymerase activity ( dnaQ )
GO:0004518
;
nuclease activity ( dnaQ )
GO:0004527
;
exonuclease activity ( dnaQ )
GO:0005515
;
protein binding ( dnaQ )
GO:0005622
;
intracellular ( dnaQ )
GO:0006260
;
DNA replication ( dnaQ )
GO:0016740
;
transferase activity ( dnaQ )
GO:0016779
;
nucleotidyltransferase activity ( dnaQ )
GO:0016787
;
hydrolase activity ( dnaQ )
GO:0030145
;
manganese ion binding ( dnaQ )
GO:0046872
;
metal ion binding ( dnaQ )
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PID
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1786409
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EC number
(KEGG Pathway)
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2.7.7.7
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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DP3E_ECOLI
(
P03007
)
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- Protein name
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DNA polymerase III, epsilon chain
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- Synonyms
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EC 2.7.7.7
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- Gene name
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Name=dnaQ; Synonyms=mutD; OrderedLocusNames=b0215;
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MMBR References
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J Biol Chem. 1984;259:5567-5573
The interaction of DNA polymerase III and the product of the Escherichia coli mutator gene, mutD.
DiFrancesco, R., S. K. Bhatnagar, A. Brown, and M. J. Bessman.
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Proc Natl Acad Sci U S A. 1983;80:2189-2192
Mutator strains of Escherichia coli, mutD and dnaQ, with defective exonucleolytic editing by DNA polymerase III holoenzyme.
Echols, H., C. Lu, and P. M. J. Burgers.
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J Bacteriol. 1997;179:7435-7445
Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity.
Fijalkowska, I. J., R. L. Dunn, and R. M. Schaaper.
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J Bacteriol. 1968;96(4):975-80.
Selection of a mutant of Escherichia coli which has high mutation rates.
Helling RB.
(
4879569
)
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Proc Natl Acad Sci U S A. 1981;78:3770-3774
Identification of the dnaQ gene product and location of the structural gene for RNase H of Escherichia coli by cloning of the genes.
Horiuchi, T., H. Maki, M. Maruyana, and M. Sekiguchi.
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Annu Rev Biochem. 1995;64:171-200.
DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.
Kelman Z, O'Donnell M.
(
7574479
)
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Proc Natl Acad Sci U S A. 1983;80:7137-7141
Structure and expression of the dnaQ mutator and the RNase H genes of Escherichia coli: overlap of the promoter regions.
Maki, H., T. Horiuchi, and M. Sekiguchi.
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J Mol Biol. 1983;167(4):757-71.
A dominant (mutD5) and a recessive (dnaQ49) mutator of Escherichia coli.
Maruyama M, Horiuchi T, Maki H, Sekiguchi M.
(
6224021
)
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J Biol Chem. 1985;260:7122-7125
Transcriptional organization of the convergent overlapping dnaQ-rnh genes of Escherichia coli.
Nomura, T., H. Aiba, and A. Ishihama.
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Mol Microbiol. 1997;25(2):237-46.
The ssb-113 allele suppresses the dnaQ49 mutator and alters DNA supercoiling in Escherichia coli.
Quinones A, Neumann S.
(
9282736
)
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Proc Natl Acad Sci U S A. 1983;80:7085-7089
Identification of the E-subunit of Escherichia coli DNA polymerase III holoenzyme as the dnaQ gene product: a fidelity subunit for DNA replication.
Scheurmann, R., S. Tam, P. M. J. Burgers, C. Lu, and H. Echols.
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J Bacteriol. 1967;94(1):38-47.
Mutator gene of Escherichia coli B.
Siegel EC, Bryson V.
(
5338974
)
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Mol Gen Genet. 1986;205(1):9-13.
Structure and function of dnaQ and mutD mutators of Escherichia coli.
Takano K, Nakabeppu Y, Maki H, Horiuchi T, Sekiguchi M.
(
3540531
)
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J Bacteriol. 1992;174:7750-7756
The gene coding for 3-deoxy-manno-octulosonic acid transferase and the rfaQ gene are transcribed from divergently arranged promoters in Escherichia coli.
Clementz, T.
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J Mol Biol. 1986;190(1):113-7.
DNA sequence and coding properties of mutD(dnaQ) a dominant Escherichia coli mutator gene.
Cox EC, Horner DL.
(
3023634
)
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Genetics 1974;77:169-184
Selection for high mutation rates in chemostats.
Cox, E. C., and T. Gibson.
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| Sequences |
Amino acid
FASTA format
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0001 MSTAITRQIV LDTETTGMNQ IGAHYEGHKI IEIGAVEVVN RRLTGNNFHV YLKPDRLVDP EAFGVHGIAD
0071 EFLLDKPTFA EVADEFMDYI RGAELVIHNA AFDIGFMDYE FSLLKRDIPK TNTFCKVTDS LAVARKMFPG
0141 KRNSLDALCA RYEIDNSKRT LHGALLDAQI LAEVYLAMTG GQTSMAFAME GETQQQQGEA TIQRIVRQAS
0211 KLRVVFATDE EIAAHEARLD LVQKKGGSCL WRA
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 CGAACCATCG GTGAAAATTT CTACCTGTTT
-070 AAGCATCTCT GGTAGACTTC CTGTAATTGA ATCGAACTGT AAAACGACAA GTCTGACATA AATGACCGCT
0001 atgagcactg caattacacg ccagatcgtt ctcgataccg aaaccaccgg tatgaaccag attggtgcgc
0071 actatgaagg ccacaagatc attgagattg gtgccgttga agtggtgaac cgtcgcctga cgggcaataa
0141 cttccatgtt tatctcaaac ccgatcggct ggtggatccg gaagcctttg gcgtacatgg tattgccgat
0211 gaatttttgc tcgataagcc cacgtttgcc gaagtagccg atgagttcat ggactatatt cgcggcgcgg
0281 agttggtgat ccataacgca gcgttcgata tcggctttat ggactacgag ttttcgttgc ttaagcgcga
0351 tattccgaag accaatactt tctgtaaggt caccgatagc cttgcggtgg cgaggaaaat gtttcccggt
0421 aagcgcaaca gcctcgatgc gttatgtgct cgctacgaaa tagataacag taaacgaacg ctgcacgggg
0491 cattactcga tgcccagatc cttgcggaag tttatctggc gatgaccggt ggtcaaacgt cgatggcttt
0561 tgcgatggaa ggagagacac aacagcaaca aggtgaagca acaattcagc gcattgtacg tcaggcaagt
0631 aagttacgcg ttgtttttgc gacagatgaa gagattgcag ctcatgaagc ccgtctcgat ctggtgcaga
0701 agaaaggcgg aagttgcctc tggcgagcat aaATACCTGT GAAAGGCGCT AAAAATAGCG ACTTGGGCGA
0771 TTTTTGCAGC AAACGATTCA AAAGATGAGA AAAACCGTTG ACGAAGGTCG AGGCAATCCG TA
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