|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCL89
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
rnt
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
rnt
Search strains by all related name:
rnt b1652 ECK1648 JW1644
|
General information (Go to Linear View: )
|
|
Gene Name
|
rnt
|
|
Alternative name
|
b1652,ECK1648,JW1644
|
|
Location, Length
|
1,726,371 - 1,727,018
(
+
)
;
37.21
min
;
648
(bp) ,
215
(aa)
|
|
Product
|
ribonuclease T (RNase T)
|
|
Operon Name
|
rnt-lhr
|
|
Note
|
RNase T, degrades tRNA; GO_process: GO:0006401 - RNA catabolic process; GO_process: GO:0006308 - DNA catabolic process
|
|
Function
|
enzyme; Degradation of RNA
|
|
Gene Ontology
|
GO:0003676
;
nucleic acid binding ( rnt )
GO:0004518
;
nuclease activity ( rnt )
GO:0004527
;
exonuclease activity ( rnt )
GO:0004540
;
ribonuclease activity ( rnt )
GO:0005515
;
protein binding ( rnt )
GO:0005622
;
intracellular ( rnt )
GO:0006396
;
RNA processing ( rnt )
GO:0008033
;
tRNA processing ( rnt )
GO:0016787
;
hydrolase activity ( rnt )
GO:0016896
;
exoribonuclease activity, producing 5'-phosphomonoesters ( rnt )
|
|
PID
|
1787941
|
EC number
(KEGG Pathway)
|
3.1.13.-
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
RNT_ECOLI
(
P30014
;
P76896
)
|
|
- Protein name
|
Ribonuclease T
|
|
- Synonyms
|
EC 3.1.13.-, Exoribonuclease T, RNase T
|
|
- Gene name
|
Name=rnt; OrderedLocusNames=b1652;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
rnt
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, 2.1 A Crystal Structure Of Pseudomonas Aeruginosa Rnase T (Ribonuclease T)
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Ribonuclease T
|
|
PSI-BLAST
|
Ribonuclease T
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
ribonuclease T [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
J Biol Chem. 1992;267:25609-25613
Sequence and transcriptional analysis of the Escherichia coli rnt gene encoding RNase T.
Huang, S., and M. P. Deutscher.
|
J Bacteriol. 1991;173(4):1376-81.
RNase T affects Escherichia coli growth and recovery from metabolic stress.
Padmanabha KP, Deutscher MP.
(
1704882
)
|
J Bacteriol. 1995;177:5393-5400
The gene for the longest known Escherichia coli protein is a member of helicase superfamily II.
Reuven, N. B., E. V. Koonin, K. E. Rudd, and M. P. Deutscher.
|
J Bacteriol. 1989;171(10):5736-7.
Localization of the Escherichia coli rnt gene encoding RNase T by using a combination of physical and genetic mapping.
Case LM, Chen XN, Deutscher MP.
(
2676990
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSDNAQLTGL CDRFRGFYPV VIDVETAGFN AKTDALLEIA AITLKMDEQG WLMPDTTLHF HVEPFVGANL
0071 QPEALAFNGI DPNDPDRGAV SEYEALHEIF KVVRKGIKAS GCNRAIMVAH NANFDHSFMM AAAERASLKR
0141 NPFHPFATFD TAALAGLALG QTVLSKACQT AGMDFDSTQA HSALYDTERT AVLFCEIVNR WKRLGGWPLS
0211 AAEEV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TCCTGCCGGG CGTGAACTCA TCGCGCCCGC
-070 ATCTTTACTG CATCGACAAG TAATATTTGT CATAATGCGC GCTGCAATTT ATCCGTATTA AGAGAATCAG
0001 atgtccgata acgctcaact taccggtctg tgcgaccgtt ttcgtggttt ttatcctgtt gtgatcgatg
0071 ttgaaacagc cggatttaac gccaaaaccg atgcgctgct tgagattgcc gccatcaccc tgaaaatgga
0141 tgaacaaggc tggctgatgc cggacaccac attacatttc cacgtcgaac catttgtcgg cgcaaatttg
0211 caaccagaag ccctcgcctt caacggcatt gacccgaacg atcccgatcg cggcgcggtc agcgaatacg
0281 aggcgctgca cgaaattttt aaagttgtac gtaaaggtat taaagcgagc ggctgtaacc gcgccattat
0351 ggtggcgcac aatgccaatt ttgatcacag ctttatgatg gccgccgcag aacgcgcctc actgaaacgt
0421 aacccgttcc accctttcgc cacttttgac actgctgcac tggccgggct ggcactcgga caaaccgtat
0491 tgtcaaaggc ttgccagacc gctggcatgg acttcgacag cacccaggcg cactccgcgc tgtacgacac
0561 cgaacgcact gctgtgctgt tttgtgaaat cgtcaaccgc tggaaacgtc tgggaggctg gccgctatct
0631 gccgccgaag aggtgtaaTC GAGTCGATGC CTGATGACAT GCAATGATTC AGGCATCTAT AGTGAGGCTA
0701 TTCCACGCAT CCTGCATGAT ATTCACGGGG AATAGCGTTA ATGGCAGA
|
