MG1655
W3110
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Gene Report : potA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR63 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  potA
Related strains
     Strains Search Search strains by gene name:  potA   Search strains by all related name:  potA b1126 ECK1112 JW1112

General information  (Go to Linear View:)
 Gene Name potA  
 Alternative name b1126,ECK1112,JW1112  
 Location, Length 1,183,681 - 1,184,817 (  -  ) ;   25.51 min ; 1137 (bp) ,   378 (aa) Go to Linear View
 Product polyamine transporter subunit  
 Operon Name potABCD  
 Note ATP-binding component of spermidine/putrescine transport; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006596 - polyamine biosynthetic process; GO_process: GO:0009310 - amine catabolic process  
 Function transport; Transport of small molecules: Amino acids, amines  
 Gene Ontology GO:0000166 ; nucleotide binding ( potA )
GO:0005215 ; transporter activity ( potA )
GO:0005515 ; protein binding ( potA )
GO:0005524 ; ATP binding ( potA )
GO:0005886 ; plasma membrane ( potA )
GO:0006810 ; transport ( potA )
GO:0015417 ; polyamine-transporting ATPase activity ( potA )
GO:0015846 ; polyamine transport ( potA )
GO:0016020 ; membrane ( potA )
GO:0016787 ; hydrolase activity ( potA )
GO:0016820 ; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances ( potA )
GO:0016887 ; ATPase activity ( potA )
GO:0017111 ; nucleoside-triphosphatase activity ( potA )
GO:0043190 ; ATP-binding cassette (ABC) transporter complex ( potA )
 PID 1787370  
 EC number
  (KEGG Pathway)   		 
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) POTA_ECOLI ( P23858 )
    -  Protein name Spermidine/putrescine transport ATP-binding protein potA.  
    -  Synonyms  
    -  Gene name OrderedLocusNames=b1126, c1477, z1831, ECs1571, SF1128, S1208;  

 Linear View (Whole Mode)
View Location
   1175.0  –  1200.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP potA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of The Atp-Binding Cassette Of Multisugar Transporter From Pyrococcus Horikoshii Ot3 Complexed With Atp  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Spermidine/putrescine import ATP-binding protein potA  
         PSI-BLAST Spermidine/putrescine import ATP-binding protein potA
    nr     (database updated : 2007.02.20 )
         BLAST putrescine/spermidine ABC transporter ATPase [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    ATP_GTP_A    ABC_TRANSPORTER     

Other Cross-Reference
    COG COG3842E 
    EcoCyc potA 

MMBR References
J Biol Chem. 1991;266:20928-20933
Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome.
Furuchi, T., K. Kashiwagi, H. Kobayashi, and K. Igarashi.
J Biol Chem. 1995;270:25377-25382
Spermidine-preferential uptake system in Escherichia coli. ATP hydrolysis by PotA protein and its association with membrane.
Kashiwagi, K., H. Endo, H. Kobayashi, K. Takio, and K. Igarashi.
J Biol Chem. 1993;268:19358-19363
Functions of potA and potD proteins in spermidine-preferential uptake system in Escherichia coli.
Kashiwagi, K., S. Miyamoto, E. Nukui, H. Kobayashi, and K. Igarashi.
J Bacteriol. 1993;175(23):7745-6.
Physical mapping of the Escherichia coli pepT and potABCD genes.
Lombardo MJ, Miller CG, Rudd KE. ( 8244951 )

Sequences
Amino acid
FASTA format 
0001 MGQSKKLNKQ PSSLSPLVQL AGIRKCFDGK EVIPQLDLTI NNGEFLTLLG PSGCGKTTVL RLIAGLETVD 
0071 SGRIMLDNED ITHVPAENRY VNTVFQSYAL FPHMTVFENV AFGLRMQKTP AAEITPRVME ALRMVQLETF 
0141 AQRKPHQLSG GQQQRVAIAR AVVNKPRLLL LDESLSALDY KLRKQMQNEL KALQRKLGIT FVFVTHDQEE 
0211 ALTMSDRIVV MRDGRIEQDG TPREIYEEPK NLFVAGFIGE INMFNATVIE RLDEQRVRAN VEGRECNIYV 
0281 NFAVEPGQKL HVLLRPEDLR VEEINDDNHA EGLIGYVRER NYKGMTLESV VELENGKMVM VSEFFNEDDP 
0351 DFDHSLDQKM AINWVESWEV VLADEEHK
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             ggcgattcaa gcggtttgcc gtagaattac 
-070 cgccctcatt aagagtcacc aaggtggtta accacaaacc ccgcatcggt aagccatccg ttgcgtttac 
0001 atgggacaga gtaaaaaatt gaataaacaa ccgagttcgc tttcaccgct ggtgcaattg gcgggaattc 
0071 gcaaatgctt tgatggtaaa gaggtcattc cccagctgga tctgactatc aacaatggcg agttcctcac 
0141 gctgcttggc ccttctggct gcggtaaaac aaccgttctt cgcctgattg caggtctgga aactgttgat 
0211 tccggacgca tcatgctgga taacgaggac atcacccacg ttccggcgga aaaccgctat gtgaacactg 
0281 ttttccaaag ctacgcactt ttcccccaca tgaccgtgtt cgaaaatgtg gcctttgggt tgcgcatgca 
0351 aaaaaccccc gctgctgaaa ttacgccccg cgtgatggaa gccctgcgga tggtgcagtt ggaaaccttc 
0421 gctcaacgca aaccgcatca gctctctggt ggtcaacagc aacgcgtcgc cattgctcgc gcggtggtta 
0491 acaagcctcg tctgttgttg ctggatgagt cgctctcagc gctggattac aaactgcgta agcaaatgca 
0561 gaacgagctg aaagcgttac agcgtaagct tggcattaca ttcgtctttg tgactcacga ccaggaagaa 
0631 gcactcacca tgtcagacag gattgtggtg atgcgcgatg gtcgcattga gcaagacggc acgccgcgtg 
0701 aaatctacga agagccgaaa aacctgtttg ttgccggctt cattggcgaa atcaatatgt ttaacgccac 
0771 tgtcatcgaa cgtctagacg agcagcgcgt acgcgccaac gttgaaggcc gcgaatgtaa tatctacgtt 
0841 aacttcgccg ttgaaccggg gcaaaaactg catgttctgc tgcgcccgga agacttacgt gttgaagaga 
0911 ttaacgacga caaccacgct gaagggctga ttggttacgt tcgcgagcgt aactacaaag gcatgacgct 
0981 ggagtcggtt gttgaactgg aaaatggcaa gatggtgatg gtcagcgaat tcttcaatga agacgatcct 
1051 gactttgacc actctctcga ccaaaaaatg gccattaatt gggtagaaag ctgggaggtc gtactggctg 
1121 atgaagaaca caagtaagtt ccagaatgta gtgattgtca ctattgtcgg ttggcttgtg ttgtttgtct 
1191 ttctgcccaa cctgatgatc attggcacca gctttttgac ccgcgac