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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Biochemistry. 1997;36(20):6223-9.
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS.
(
9166795
)
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Deletion
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OCR30,31-3 (BK2-1 / AK1-1)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
murF
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Related strains
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Strains Search
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Search strains by gene name:
murF
Search strains by all related name:
murF b0086 ECK0087 JW0084 mra
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General information (Go to Linear View: )
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Gene Name
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murF
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Alternative name
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b0086,ECK0087,JW0084,mra
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Location, Length
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94,650 - 96,008
(
+
)
;
2.04
min
;
1359
(bp) ,
452
(aa)
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Product
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UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase
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Operon Name
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mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC
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Note
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GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0000270 - peptidoglycan metabolic process
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Function
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enzyme; Murein sacculus, peptidoglycan,1.6.7 metabolism; macromolecules (cellular constituent) biosynthesis; peptidoglycan (murein),1.7.34 metabolism; central intermediary metabolism; peptidoglycan (murein),1.7.34 metabolism; central intermediary metaboli
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Gene Ontology
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GO:0000166
;
nucleotide binding ( murF )
GO:0005524
;
ATP binding ( murF )
GO:0005737
;
cytoplasm ( murF )
GO:0007047
;
cell wall organization and biogenesis ( murF )
GO:0007049
;
cell cycle ( murF )
GO:0008360
;
regulation of cell shape ( murF )
GO:0008766
;
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ( murF )
GO:0009058
;
biosynthetic process ( murF )
GO:0009252
;
peptidoglycan biosynthetic process ( murF )
GO:0009273
;
peptidoglycan-based cell wall biogenesis ( murF )
GO:0016874
;
ligase activity ( murF )
GO:0047480
;
UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity ( murF )
GO:0051301
;
cell division ( murF )
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PID
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1786274
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EC number
(KEGG Pathway)
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6.3.2.10
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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murF
(
EG10622
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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MURF_ECOLI
(
P11880
;
O07100; P77636
)
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- Protein name
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UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
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- Synonyms
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EC 6.3.2.10, UDP-MurNAc-pentapeptide synthetase, D-alanyl-D- alanine-adding enzyme
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- Gene name
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Name=murF; Synonyms=mra; OrderedLocusNames=b0086;
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MMBR References
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Biochemistry. 1996;35(50):16264-9.
Kinetic mechanism of the Escherichia coli UDPMurNAc-tripeptide D-alanyl-D-alanine-adding enzyme: use of a glutathione S-transferase fusion.
Anderson MS, Eveland SS, Onishi HR, Pompliano DL.
(
8973200
)
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Biochemistry. 1997;36(20):6223-9.
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS.
(
9166795
)
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J Bacteriol. 1989;171(11):6379-82.
Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes.
Jung HK, Ishino F, Matsuhashi M.
(
2553679
)
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J Bacteriol. 1989;171(11):6126-34.
Organization of the murE-murG region of Escherichia coli: identification of the murD gene encoding the D-glutamic-acid-adding enzyme.
Mengin-Lecreulx D, Parquet C, Desviat LR, Pla J, Flouret B, Ayala JA, van Heijenoort J.
(
2681153
)
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Nucleic Acids Res. 1989;17(13):5379
Nucleotide sequence of the murF gene encoding the UDP-MurNAc-pentapeptide synthetase of Escherichia coli.
Parquet C, Flouret B, Mengin-Lecreulx D, van Heijenoort J.
(
2668880
)
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| Sequences |
Amino acid
FASTA format
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0001 MISVTLSQLT DILNGELQGA DITLDAVTTD TRKLTPGCLF VALKGERFDA HDFADQAKAG GAGALLVSRP
0071 LDIDLPQLIV KDTRLAFGEL AAWVRQQVPA RVVALTGSSG KTSVKEMTAA ILSQCGNTLY TAGNLNNDIG
0141 VPMTLLRLTP EYDYAVIELG ANHQGEIAWT VSLTRPEAAL VNNLAAAHLE GFGSLAGVAK AKGEIFSGLP
0211 ENGIAIMNAD NNDWLNWQSV IGSRKVWRFS PNAANSDFTA TNIHVTSHGT EFTLQTPTGS VDVLLPLPGR
0281 HNIANALAAA ALSMSVGATL DAIKAGLANL KAVPGRLFPI QLAENQLLLD DSYNANVGSM TAAVQVLAEM
0351 PGYRVLVVGD MAELGAESEA CHVQVGEAAK AAGIDRVLSV GKQSHAISTA SGVGEHFADK TALITRLKLL
0421 IAEQQVITIL VKGSRSAAME EVVRALQENG TC
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 TGGTCGCGGG CAAAGGCCAT GAAGATTACC
-070 AGATTGTTGG CAATCAGCGT CTGGACTACT CCGATCGCGT CACGGTGGCG CGTCTGCTGG GGGTGATTGC
0001 atgattagcg taacccttag ccaacttacc gacattctca acggtgaact gcaaggtgca gatatcaccc
0071 ttgatgctgt aaccactgat acccgaaaac tgacgccggg ctgcctgttt gttgccctga aaggcgaacg
0141 ttttgatgcc cacgattttg ccgaccaggc gaaagctggc ggcgcaggcg cactactggt tagccgtccg
0211 ctggacatcg acctgccgca gttaatcgtc aaggatacgc gtctggcgtt tggtgaactg gctgcatggg
0281 ttcgccagca agttccggcg cgcgtggttg ctctgacggg gtcctccggc aaaacctccg ttaaagagat
0351 gacggcggcg attttaagcc agtgcggcaa cacgctttat acggcaggca atctcaacaa cgacatcggt
0421 gtaccgatga cgctgttgcg cttaacgccg gaatacgatt acgcagttat tgaacttggc gcgaaccatc
0491 agggcgaaat agcctggact gtgagtctga ctcgcccgga agctgcgctg gtcaacaacc tggcagcggc
0561 gcatctggaa ggttttggct cgcttgcggg tgtcgcgaaa gcgaaaggtg aaatctttag cggcctgccg
0631 gaaaacggta tcgccattat gaacgccgac aacaacgact ggctgaactg gcagagcgta attggctcac
0701 gcaaagtgtg gcgtttctca cccaatgccg ccaacagcga tttcaccgcc accaatatcc atgtgacctc
0771 gcacggtacg gaatttaccc tacaaacccc aaccggtagc gtcgatgttc tgctgccgtt gccggggcgt
0841 cacaatattg cgaatgcgct ggcagccgct gcgctctcca tgtccgtggg cgcaacgctt gatgctatca
0911 aagcggggct ggcaaatctg aaagctgttc caggccgtct gttccccatc caactggcag aaaaccagtt
0981 gctgctcgac gactcctaca acgccaatgt cggttcaatg actgcagcag tccaggtact ggctgaaatg
1051 ccgggctacc gcgtgctggt ggtgggcgat atggcggaac tgggcgctga aagcgaagcc tgccatgtac
1121 aggtgggcga ggcggcaaaa gctgctggta ttgaccgcgt gttaagcgtg ggtaaacaaa gccatgctat
1191 cagcaccgcc agcggcgttg gcgaacattt tgctgataaa actgcgttaa ttacgcgtct taaattactg
1261 attgctgagc aacaggtaat tacgatttta gttaagggtt cacgtagtgc cgccatggaa gaggtagtac
1331 gcgctttaca ggagaatggg acatgttagT TTGGCTGGCC GAACATTTGG TCAAATATTA TTCCGGCTTT
1401 AACGTCTTTT CCTATCTGAC GTTTCGCGCC ATCGTCAGCC TGCTGACCGC GCTGTTCAT
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