MG1655
W3110
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Gene Report : wcaF
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 16-1-2 (LD)  ,  DE(sbcB-rfb)202  ,  DE(his-dcd)453  ,  OCL74-2,75-5 (LD 16-1-2_CRS) (D)  ,  OCL74-2,75-6 (LD 16-1-2_P/O) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  wcaF
Related strains
     Strains Search Search strains by gene name:  wcaF   Search strains by all related name:  wcaF b2054 ECK2048 f182 JW2039

General information  (Go to Linear View:)
 Gene Name wcaF  
 Alternative name b2054,ECK2048,f182,JW2039  
 Location, Length 2,126,364 - 2,126,912 (  -  ) ;   45.83 min ; 549 (bp) ,   182 (aa) Go to Linear View
 Product putative acyl transferase  
 Operon Name wcaCDEF-gmd-fcl-gmm-wcaI-cpsBG-wcaJ-wzxC  
 Note putative transferase; GO_process: GO:0009242 - colanic acid biosynthetic process  
 Function putative enzyme; Not classified  
 Gene Ontology GO:0008415 ; acyltransferase activity ( wcaF )
GO:0009103 ; lipopolysaccharide biosynthetic process ( wcaF )
GO:0016740 ; transferase activity ( wcaF )
 PID 1788367  
 EC number
  (KEGG Pathway)   		 
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) WCAF_ECOLI ( P71240 ; P76383 )
    -  Protein name Putative colanic acid biosynthesis acetyltransferase wcaF  
    -  Synonyms EC 2.3.1.-  
    -  Gene name Name=wcaF; OrderedLocusNames=b2054, SF2117, S2240;  

 Linear View (Whole Mode)
View Location
   2115.0  –  2140.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP wcaF (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Maltose Transacetylase From Geobacillus Kaustophilus  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Putative colanic acid biosynthesis acetyltransferase wcaF  
         PSI-BLAST Bifunctional protein glmU [Includes: UDP-N-acetylglucosamine pyrophosphorylase
    nr     (database updated : 2007.02.20 )
         BLAST predicted acyl transferase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    C5_MTASE_2    HEXAPEP_TRANSFERASES     

Other Cross-Reference
    COG COG0110R 
    EcoCyc wcaF 

Sequences
Amino acid
FASTA format 
0001 MQDLSGFSVP KGFRGGNAIK VQLWWAVQAT IFAWSPQVLY RWRAFLLRLF GAKIGKNVVI RPSVKITYPW 
0071 KLTLGDYAWV GDDVNLYTLG EITIGAHSVI SQKSYLCTGS HDHASQHFTI NATPIVIGEK CWLATDVFVA 
0141 PGVTIGDGTV VGARSSVFKS LPANVVCRGN PAVVIRERVE TE
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             tgtgcctggc ttttgggctg aattatcctg 
-070 gcatttacgc caacgtacta cctcaaagac gaaagcctta tataacaaag tctgaatata aggaaaacca 
0001 atgcaagatt taagcggttt ctcggtgccg aaagggttcc ggggcggcaa cgctattaaa gtgcaattat 
0071 ggtgggcagt acaggcaaca atatttgcct ggtcgccaca agtattgtat cgctggcggg cttttttatt 
0141 acgtttattc ggagcaaaaa taggaaaaaa cgtagttatt cgtccgtcag taaaaattac ctatccgtgg 
0211 aaattaacct taggtgatta cgcgtgggtc ggcgatgacg tcaatttata taccctcggt gaaataacca 
0281 ttggcgcaca ttcggtgata tcgcaaaaaa gttatttatg caccggtagc cacgaccatg caagtcaaca 
0351 tttcaccatt aacgccacgc ctattgtgat tggcgagaaa tgctggctgg caaccgatgt ctttgttgcc 
0421 ccaggcgtca caatcggcga cggcaccgtc gtgggtgcac gaagcagtgt ttttaaatcg cttccggcaa 
0491 atgtggtttg ccgggggaat cccgcagtgg tgatacgcga acgcgttgaa actgaataaa ttcaaaaata 
0561 cagaggaata atacatgtca aaagtcgctc tcatcaccgg tgtaaccgga caagacggtt cttacctggc 
0631 agagtttctg ctggaaaaa