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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(tonB-cysB)206
,
OCR68,69-1
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
trpA
|
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Related strains
|
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Strains Search
|
Search strains by gene name:
trpA
Search strains by all related name:
trpA b1260 ECK1254 JW1252 try tryp
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General information (Go to Linear View: )
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Gene Name
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trpA
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Alternative name
|
b1260,ECK1254,JW1252,try,tryp
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Location, Length
|
1,314,440 - 1,315,246
(
-
)
;
28.33
min
;
807
(bp) ,
268
(aa)
|
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Product
|
tryptophan synthase, alpha subunit
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Operon Name
|
trpLEDCBA
|
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Note
|
tryptophan synthase, alpha protein; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0000162 - tryptophan biosynthetic process
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Function
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enzyme; Amino acid biosynthesis: Tryptophan
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Gene Ontology
|
GO:0000162
;
tryptophan biosynthetic process ( trpA )
GO:0003824
;
catalytic activity ( trpA )
GO:0004834
;
tryptophan synthase activity ( trpA )
GO:0006568
;
tryptophan metabolic process ( trpA )
GO:0008152
;
metabolic process ( trpA )
GO:0008652
;
amino acid biosynthetic process ( trpA )
GO:0009073
;
aromatic amino acid family biosynthetic process ( trpA )
GO:0016829
;
lyase activity ( trpA )
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PID
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1787514
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EC number
(KEGG Pathway)
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4.2.1.20
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
trpA
(
EG11024
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
TRPA_ECOLI
(
P00928
;
Q47669
)
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- Protein name
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Tryptophan synthase alpha chain
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- Synonyms
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EC 4.2.1.20
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- Gene name
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Name=trpA; OrderedLocusNames=b1260, SF1263, S1349;
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MMBR References
|
Gene. 1980;9(1-2):69-85.
Construction of new cloning vehicles with genes of the tryptophan operon of Escherichia coli as genetic markers.
Enger-Valk BE, Heyneker HL, Oosterbaan RA, Pouwels PH.
(
6247248
)
|
J Mol Biol. 1969;44(1):117-27.
Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.
Gottesman S, Beckwith JR.
(
4897798
)
|
J Biol Chem. 1967;242:5442-5446
The amino acid sequence of the A protein (alpha subunit) of the tryptophan synthetase of Escherichia coli.
Guest, J. R., G. R. Drapeau, B. C. Carlton, and C. Yanofsky.
|
J Mol Biol. 1969;39(1):159-79.
Operator mutants of the tryptophan operon in Escherichia coli.
Hiraga S.
(
4938813
)
|
J Bacteriol. 1971;107(1):8-15.
Regulation of tyrosine biosynthesis in Escherichia coli K-12: isolation and characterization of operator mutants.
Mattern IE, Pittard J.
(
4397929
)
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Proc Natl Acad Sci U S A. 1979;76:5244-5248
Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison.
Nichols, B., and C. Yanofsky.
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Mol Gen Genet. 1992;233(1-2):129-35.
Selection and analysis of non-interactive mutants in the Escherichia coli tryptophan synthase alpha subunit.
Swift S, Kuhn J, Stewart GS.
(
1603055
)
|
Cell. 1980;19(4):829-36.
Deletions of distal sequence after termination of transcription at the end of the tryptophan operon in E. coli.
Wu AM, Chapman AB, Platt T, Guarente LP, Beckwith J.
(
6991123
)
|
Proc Natl Acad Sci U S A. 1981;78:2913-2917
Tandem termination sites in the tryptophan operon of Escherichia coli.
Wu, A. M., G. E. Christie, and T. Platt.
|
Nucleic Acids Res. 1981;9:6647-6668
The complete nucleotide sequence of the tryptophan operon of E. coli.
Yanofsky, C., T. Platt, I. P. Crawford, B. Nichols, G. E. Christie, H. Horowitz, M. VanCleemput, and A. M. Wu.
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| Sequences |
Amino acid
FASTA format
|
0001 MERYESLFAQ LKERKEGAFV PFVTLGDPGI EQSLKIIDTL IEAGADALEL GIPFSDPLAD GPTIQNATLR
0071 AFAAGVTPAQ CFEMLALIRQ KHPTIPIGLL MYANLVFNKG IDEFYAQCEK VGVDSVLVAD VPVEESAPFR
0141 QAALRHNVAP IFICPPNADD DLLRQIASYG RGYTYLLSRA GVTGAENRAA LPLNHLVAKL KEYNAAPPLQ
0211 GFGISAPDQV KAAIDAGAAG AISGSAIVKI IEQHINEPEK MLAALKVFVQ PMKAATRS
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 acccggataa agagcagcta ctggtggtta
-070 acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg aaagcacgag gggaaatctg
0001 atggaacgct acgaatctct gtttgcccag ttgaaggagc gcaaagaagg cgcattcgtt cctttcgtca
0071 cgctcggtga tccgggcatt gagcagtcat tgaaaattat cgatacgcta attgaagccg gtgctgacgc
0141 gctggagtta ggtatcccct tctccgaccc actggcggat ggcccgacga ttcaaaacgc cactctgcgc
0211 gcctttgcgg caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga
0281 ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt tttatgccca
0351 gtgcgaaaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg aagagtccgc gcccttccgc
0421 caggccgcgt tgcgtcataa tgtcgcacct atcttcatct gcccgccaaa tgccgatgac gacctgctgc
0491 gccagatagc ctcttacggt cgtggttaca cctatttgct gtcacgagca ggcgtgaccg gcgcagaaaa
0561 ccgcgccgcg ttacccctca atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag
0631 ggatttggta tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg
0701 gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg cactgaaagt
0771 ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc agttccgctg gcggcatttt
0841 aactttcttt aatgaagccg gaaaaatcct aaattcattt aatatttatc tttttaccgt ttcgctt
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