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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1987;169(2):605-11.
Fatty acid metabolism in sn-glycerol-3-phosphate acyltransferase (plsB) mutants.
Cooper CL, Jackowski S, Rock CO.
(
3542964
)
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|
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Deletion
|
OCR12
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
plsB
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Related strains
|
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Strains Search
|
Search strains by gene name:
plsB
Search strains by all related name:
plsB b4041 ECK4033 f827 JW4001
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General information (Go to Linear View: )
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Gene Name
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plsB
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Alternative name
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b4041,ECK4033,f827,JW4001
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Location, Length
|
4,252,066 - 4,254,489
(
-
)
;
91.65
min
;
2424
(bp) ,
807
(aa)
|
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Product
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glycerol-3-phosphate O-acyltransferase
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Operon Name
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plsB
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Note
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glycerol-3-phosphate acyltransferase; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0008654 - phospholipid biosynthetic process
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Function
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enzyme; Macromolecule synthesis, modification: Phospholipids
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Gene Ontology
|
GO:0004366
;
glycerol-3-phosphate O-acyltransferase activity ( plsB )
GO:0005886
;
plasma membrane ( plsB )
GO:0008152
;
metabolic process ( plsB )
GO:0008415
;
acyltransferase activity ( plsB )
GO:0008654
;
phospholipid biosynthetic process ( plsB )
GO:0016020
;
membrane ( plsB )
GO:0016740
;
transferase activity ( plsB )
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PID
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87082362
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EC number
(KEGG Pathway)
|
2.3.1.15
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
plsB
(
EG10740
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
|
PLSB_ECOLI
(
P00482
;
Q9S683
)
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- Protein name
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Glycerol-3-phosphate acyltransferase
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- Synonyms
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EC 2.3.1.15, GPAT
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- Gene name
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Name=plsB; OrderedLocusNames=b4041, c5011;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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plsB
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain A, Atp Bound Gelsolin
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Glycerol-3-phosphate acyltransferase (GPAT)
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PSI-BLAST
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Glycerol-3-phosphate acyltransferase
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nr
(database updated :
2007.02.20
)
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BLAST
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glycerol-3-phosphate acyltransferase [Escherichia coli CFT073]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
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MMBR References
|
J Biol Chem. 1983;258:10856-10861
The DNA sequences encoding plsB and dgk loci of Escherichia coli.
Lightner, V. A., R. M. Bell, and P. Modrich.
|
J Biol Chem. 1980;255:9413-9420
Membrane phospholipid synthesis in Escherichia coli. Cloning of a structural gene (plsB) of the sn-glycerol-3-phosphate acyltransferase.
Lightner, V. A., T. J. Larsen, P. Tailleur, G. D. Kantor, C. R. H. Raetz, R. M. Bell, and P. Modrich.
|
| Sequences |
Amino acid
FASTA format
|
0001 MSGWPRIYYK LLNLPLSILV KSKSIPADPA PELGLDTSRP IMYVLPYNSK ADLLTLRAQC LAHDLPDPLE
0071 PLEIDGTLLP RYVFIHGGPR VFTYYTPKEE SIKLFHDYLD LHRSNPNLDV QMVPVSVMFG RAPGREKGEV
0141 NPPLRMLNGV QKFFAVLWLG RDSFVRFSPS VSLRRMADEH GTDKTIAQKL ARVARMHFAR QRLAAVGPRL
0211 PARQDLFNKL LASRAIAKAV EDEARSKKIS HEKAQQNAIA LMEEIAANFS YEMIRLTDRI LGFTWNRLYQ
0281 GINVHNAERV RQLAHDGHEL VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA GPIFRRLGAF
0351 FIRRTFKGNK LYSTVFREYL GELFSRGYSV EYFVEGGRSR TGRLLDPKTG TLSMTIQAML RGGTRPITLI
0421 PIYIGYEHVM EVGTYAKELR GATKEKESLP QMLRGLSKLR NLGQGYVNFG EPMPLMTYLN QHVPDWRESI
0491 DPIEAVRPAW LTPTVNNIAA DLMVRINNAG AANAMNLCCT ALLASRQRSL TREQLTEQLN CYLDLMRNVP
0561 YSTDSTVPSA SASELIDHAL QMNKFEVEKD TIGDIIILPR EQAVLMTYYR NNIAHMLVLP SLMAAIVTQH
0631 RHISRDVLME HVNVLYPMLK AELFLRWDRD ELPDVIDALA NEMQRQGLIT LQDDELHINP AHSRTLQLLA
0701 AGARETLQRY AITFWLLSAN PSINRGTLEK ESRTVAQRLS VLHGINAPEF FDKAVFSSLV LTLRDEGYIS
0771 DSGDAEPAET MKVYQLLAEL ITSDVRLTIE SATQGEG
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 agcgccgcgc gaaacatgag cggataccac
-070 agaatttccc atgactttct gctatccttg ccgcgcattt gcattattaa ccagaggctt tacatcgttt
0001 atgtccggct ggccacgaat ttactacaaa ttactgaatt taccattaag catcctggta aaaagcaagt
0071 ctattccggc agatcctgcc ccggaactgg ggctggatac ctctcgtcca attatgtacg ttttaccgta
0141 caactcgaaa gcagatttgc tgacgttgcg cgcccagtgt ctggcacatg acttgcctga cccgttagag
0211 ccgctggaaa tcgacggcac gctactgccg cgctatgtgt tcattcacgg cgggccgcgt gtgttcacct
0281 attacacgcc gaaagaagag tctattaagc tgttccacga ctatctcgat ttgcaccgta gcaacccaaa
0351 tctggatgtg cagatggtgc cagtgtcggt gatgtttggt cgcgcgccgg ggcgtgaaaa aggcgaagtg
0421 aacccgccgc tgcgtatgct taacggcgta cagaaatttt tcgctgtact gtggctcggt cgcgacagtt
0491 ttgtgcgttt ctcgccgtca gtttcgctgc gccgtatggc ggatgaacac ggcacggata aaactatcgc
0561 tcagaaactg gcgcgcgtgg cgcgtatgca ctttgcccgt caacgtctgg ctgccgtagg cccacgtttg
0631 cctgctcgtc aggatctgtt taataagctg ctcgcctccc gcgccattgc caaagcggta gaagatgaag
0701 cgcgcagcaa aaaaatctcc catgaaaaag cgcagcagaa cgcgattgca ctgatggaag agattgcggc
0771 gaatttctct tacgagatga ttcgcctgac tgaccgtatt ctgggcttca cctggaaccg actttaccag
0841 ggcatcaacg tccataacgc tgagcgcgtt cgccagctgg cccacgacgg tcatgagctg gtatatgtgc
0911 cttgccaccg cagtcacatg gactacctgc tgctttctta cgtgctgtat caccaggggc tggtgccgcc
0981 gcatatcgcc gccgggatca acctgaattt ctggcctgcc gggccgattt tccgccgtct gggggcgttc
1051 tttattcgcc gtacgtttaa aggcaataaa ctttattcca ccgttttccg ggagtatctc ggcgaactgt
1121 tcagccgtgg ttattccgtc gagtacttcg tggaaggcgg tcgttcccgt acggggcgtt tgctggatcc
1191 gaaaactggt acgctgtcga tgaccattca ggcgatgctg cgtggcggca cgcgtccgat tacgctgatt
1261 ccgatctata tcggttatga gcacgtcatg gaagtgggta cttacgccaa agaactgcgc ggcgcgacga
1331 aagagaaaga gagcctgccg cagatgctgc gcggtttaag caagctgcgt aatctcggtc agggttacgt
1401 caacttcggt gaaccaatgc cgttgatgac ctaccttaac cagcatgtac ctgactggcg tgaatctatc
1471 gatcccatcg aagcggtgcg tccggcatgg ttaacgccga cggtcaataa tattgctgcc gatctgatgg
1541 tacgcattaa caacgcaggc gcggcaaacg ccatgaacct gtgctgtact gcgctactgg catcacgtca
1611 gcgctcactc acccgcgagc agttaaccga gcaactcaac tgctacctgg atctgatgcg caacgtgccc
1681 tactccacgg actctaccgt tccttcagcc agcgccagcg agcttatcga tcacgcgctg caaatgaaca
1751 agtttgaagt cgagaaagac acaatcggcg acatcatcat tctgccgcgc gagcaagcgg tgctgatgac
1821 ctactatcgc aacaacattg cgcatatgtt ggtgctgcct tcgctgatgg cggcaatcgt cacccagcat
1891 cgccacatct cccgcgacgt attgatggag cacgtcaatg tgctttaccc aatgctgaaa gcggagctgt
1961 tcctgcgctg ggatcgcgac gagttgccgg acgttattga tgcgctggca aatgagatgc aacgtcaggg
2031 gctgattacc ctgcaagatg atgagttgca tatcaacccg gcgcattctc gcacgctaca gctgctggcc
2101 gcaggcgcgc gcgaaacgct gcaacgttat gccatcacct tctggttgtt gagtgccaac ccgtcgatca
2171 accgcggtac gctggagaaa gagagccgca ccgtcgcgca acgtctctcc gtgctgcacg gcatcaacgc
2241 gccggagttc ttcgacaagg cggtgttcag ttctctggtg ctgacactgc gtgatgaagg gtatatcagc
2311 gatagcggcg atgccgaacc ggcagaaacg atgaaggttt atcagttgct ggcggagttg attacatcag
2381 acgtgcgttt gacgattgag agtgcgacgc agggcgaagg gtaatcagag agaattgccg gatgcggcga
2451 aaacgcctta tccggccttc cataatctgc aaattcaata aattgcgatg tgttgtgtag gcctgagaag
2521 cgcc
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