MG1655
W3110
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Gene Report : pdxJ
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL53,54-1 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  pdxJ
Related strains
     Strains Search Search strains by gene name:  pdxJ   Search strains by all related name:  pdxJ b2564 ECK2562 JW2548

General information  (Go to Linear View:)
 Gene Name pdxJ  
 Alternative name b2564,ECK2562,JW2548  
 Location, Length 2,699,020 - 2,699,751 (  -  ) ;   58.17 min ; 732 (bp) ,   243 (aa) Go to Linear View
 Product pyridoxine 5'-phosphate synthase  
 Operon Name rnc-era-recO-pdxJ-acpS  
 Note pyridoxine biosynthesis; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0008615 - pyridoxine biosynthetic process  
 Function enzyme; Biosynthesis of cofactors, carriers: Pyridoxine  
 Gene Ontology GO:0003824 ; catalytic activity ( pdxJ )
GO:0005737 ; cytoplasm ( pdxJ )
GO:0008152 ; metabolic process ( pdxJ )
GO:0008615 ; pyridoxine biosynthetic process ( pdxJ )
GO:0016740 ; transferase activity ( pdxJ )
GO:0016769 ; transferase activity, transferring nitrogenous groups ( pdxJ )
GO:0033856 ; pyridoxine 5'-phosphate synthase activity ( pdxJ )
 PID 1788917  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene pdxJ ( EG10693 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) PDXJ_ECOLI ( P24223 )
    -  Protein name Pyridoxal phosphate biosynthetic protein pdxJ  
    -  Synonyms PNP synthase  
    -  Gene name Name=pdxJ; OrderedLocusNames=b2564, z3845, ECs3430;  

 Linear View (Whole Mode)
View Location
   2690.0  –  2715.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP pdxJ (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, 1.96 A Crystal Structure Of Pyridoxine 5'-Phosphate Synthase In Complex With 1-Deoxy-D-Xylulose Phosphate  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Pyridoxal phosphate biosynthetic protein pdxJ (PNP synthase)  
         PSI-BLAST Pyridoxal phosphate biosynthetic protein pdxJ (PNP synthase)
    nr     (database updated : 2007.02.20 )
         BLAST COG0854: Pyridoxal phosphate biosynthesis protein [Escherichia coli B171]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    EcoCyc pdxJ 

MMBR References
J Bacteriol. 1992;174:1554-1567
Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations.
Lam, H. L., E. Tancula, W. B. Dempsey, and M. E. Winkler.
J Bacteriol. 1992;174:1544-1553
Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon.
Takiff, H. E., T. Baker, T. Copeland, S. M. Chen, and D. L. Court.

Sequences
Amino acid
FASTA format
0001 MAELLLGVNI DHIATLRNAR GTAYPDPVQA AFIAEQAGAD GITVHLREDR RHITDRDVRI LRQTLDTRMN 
0071 LEMAVTEEML AIAVETKPHF CCLVPEKRQE VTTEGGLDVA GQRDKMRDAC KRLADAGIQV SLFIDADEEQ 
0141 IKAAAEVGAP FIEIHTGCYA DAKTDAEQAQ ELARIAKAAT FAASLGLKVN AGHGLTYHNV KAIAAIPEMH 
0211 ELNIGHAIIG RAVMTGLKDA VAEMKRLMLE ARG

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             agccgtatct tggcggtaaa cctttaaaga 
-070 gcagggaact gttccggcag tttatgccta agcgaacggt gaaaacacat tatgaatgat gaggattgtc 
0001 atggctgaat tactgttagg cgtcaacatt gaccatatcg ctacgctgcg caacgcgcgc ggtaccgctt 
0071 acccggatcc ggtgcaggcc gcgtttattg ccgagcaggc gggagcggac ggcattaccg tgcatttacg 
0141 tgaagatcgc cgtcacatta ctgaccgcga cgtgcgcatc ctgcgtcaga cgctggatac ccgcatgaat 
0211 ctggagatgg cggtgaccga agagatgctg gcgatcgccg ttgagacgaa gccacatttt tgctgcctgg 
0281 taccggaaaa gcgtcaggaa gtaacaaccg aaggcggcct ggatgtcgca gggcagcgtg acaaaatgcg 
0351 cgatgcctgc aaacgtctgg cagatgccgg gattcaggtt tctctgttta ttgacgccga tgaagagcag 
0421 atcaaagctg cggcagaggt tggcgcaccg tttatcgaga tccacaccgg ttgctatgct gatgccaaaa 
0491 ctgacgccga acaggcgcaa gagctggcgc gtatcgccaa agccgcgacc tttgccgcaa gcctcggtct 
0561 gaaagttaac gccggacacg gtctgaccta tcacaacgtg aaagccattg ccgccatccc tgagatgcat 
0631 gaactgaata tcggtcatgc cattattggt cgtgcagtga tgaccggact gaaagatgcg gtggcagaaa 
0701 tgaagcgtct gatgctggaa gcgcgtggct aatggcaata ttaggtttag gcacggatat tgtggagatc 
0771 gctcgcatcg aagcggtgat cgcccgatcc ggtgatcgcc tggcacgccg cgtattaagc ga