|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
essential
|
|
References (PMID)
|
J Bacteriol. 1989;171(6):3025-30.
Penicillin-binding protein 2 is essential in wild-type Escherichia coli but not in lov or cya mutants.
Ogura T, Bouloc P, Niki H, D'Ari R, Hiraga S, Jaffe A.
(
2656638
)
|
|
|
Deletion
|
OCR46
(D)
,
OCR46-10
(D)
,
OCR46-11
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
mrdA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
mrdA
Search strains by all related name:
mrdA b0635 ECK0628 JW0630 pbpA
|
General information (Go to Linear View: )
|
|
Gene Name
|
mrdA
|
|
Alternative name
|
b0635,ECK0628,JW0630,pbpA
|
|
Location, Length
|
665,539 - 667,440
(
-
)
;
14.34
min
;
1902
(bp) ,
633
(aa)
|
|
Product
|
transpeptidase involved in peptidoglycan synthesis (penicillin-binding protein 2)
|
|
Operon Name
|
ybeB-rlmH-mrdAB-rlpA
|
|
Note
|
cell elongation, e phase; peptidoglycan synthetase; penicillin-binding protein 2; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0042493 - response to drug
|
|
Function
|
enzyme; Cell division
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( mrdA )
GO:0005886
;
plasma membrane ( mrdA )
GO:0007047
;
cell wall organization and biogenesis ( mrdA )
GO:0008360
;
regulation of cell shape ( mrdA )
GO:0008658
;
penicillin binding ( mrdA )
GO:0009252
;
peptidoglycan biosynthetic process ( mrdA )
GO:0009273
;
peptidoglycan-based cell wall biogenesis ( mrdA )
GO:0016020
;
membrane ( mrdA )
GO:0046677
;
response to antibiotic ( mrdA )
|
|
PID
|
1786854
|
EC number
(KEGG Pathway)
|
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
PBP2_ECOLI
(
P08150
)
|
|
- Protein name
|
Penicillin-binding protein 2
|
|
- Synonyms
|
PBP-2
|
|
- Gene name
|
OrderedLocusNames=b0635, c0726, z0781, ECs0673;
|
|
MMBR References
|
Eur J Biochem. 1986;160:231-238
Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K-12.
Asoh, S., H. Matsuzawa, F. Ishino, J. L. Strominger, M. Matsuhashi, and T. Ohta.
|
J Bacteriol. 1983;154(1):10-6.
Molecular cloning and characterization of the genes (pbpA and rodA) responsible for the rod shape of Escherichia coli K-12: analysis of gene expression with transposon Tn5 mutagenesis and protein synthesis directed by constructed plasmids.
Asoh S, Matsuzawa H, Matsuhashi M, Ohta T.
(
6300030
)
|
J Bacteriol. 1985;163(2):615-22.
Cell shape and division in Escherichia coli: experiments with shape and division mutants.
Begg KJ, Donachie WD.
(
3894330
)
|
J Bacteriol. 1980;143(2):569-81.
Defective and plaque-forming lambda transducing bacteriophage carrying penicillin-binding protein-cell shape genes: genetic and physical mapping and identification of gene products from the lip-dacA-rodA-pbpA-leuS region of the Escherichia coli chromosome.
Spratt BG, Boyd A, Stoker N.
(
6451612
)
|
J Bacteriol. 1983;155(2):847-53.
Organization and subcloning of the dacA-rodA-pbpA cluster of cell shape genes in Escherichia coli.
Stoker NG, Broome-Smith JK, Edelman A, Spratt BG.
(
6348028
)
|
J Bacteriol. 1983;155(2):854-9.
Identification of the rodA gene product of Escherichia coli.
Stoker NG, Pratt JM, Spratt BG.
(
6348029
)
|
J Bacteriol. 1980;141(1):52-7.
Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli.
Tamaki S, Matsuzawa H, Matsuhashi M.
(
6243629
)
|
J Bacteriol. 1993;175:6704-6710
Penicillin-binding protein 2 inactivation in Escherichia coli results in cell division inhibition, which is relieved by FtsZ overexpression.
Vinella, D., D. Joseleau-Petit, D. Thevenet, P. Bouloc, and R. D'Ari.
|
J Bacteriol. 1984;158(1):216-21.
Map location of the pcbA mutation and physiology of the mutant.
Bryan SK, Moses RE.
(
6325387
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKLQNSFRDY TAESALFVRR ALVAFLGILL LTGVLIANLY NLQIVRFTDY QTRSNENRIK LVPIAPSRGI
0071 IYDRNGIPLA LNRTIYQIEM MPEKVDNVQQ TLDALRSVVD LTDDDIAAFR KERARSHRFT SIPVKTNLTE
0141 VQVARFAVNQ YRFPGVEVKG YKRRYYPYGS ALTHVIGYVS KINDKDVERL NNDGKLANYA ATHDIGKLGI
0211 ERYYEDVLHG QTGYEEVEVN NRGRVIRQLK EVPPQAGHDI YLTLDLKLQQ YIETLLAGSR AAVVVTDPRT
0281 GGVLALVSTP SYDPNLFVDG ISSKDYSALL NDPNTPLVNR ATQGVYPPAS TVKPYVAVSA LSAGVITRNT
0351 TLFDPGWWQL PGSEKRYRDW KKWGHGRLNV TRSLEESADT FFYQVAYDMG IDRLSEWMGK FGYGHYTGID
0421 LAEERSGNMP TREWKQKRFK KPWYQGDTIP VGIGQGYWTA TPIQMSKALM ILINDGIVKV PHLLMSTAED
0491 GKQVPWVQPH EPPVGDIHSG YWELAKDGMY GVANRPNGTA HKYFASAPYK IAAKSGTAQV FGLKANETYN
0561 AHKIAERLRD HKLMTAFAPY NNPQVAVAMI LENGGAGPAV GTLMRQILDH IMLGDNNTDL PAENPAVAAA
0631 EDH
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 cgtgctggtc gcagagagtc tgtaccgggc
-070 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa aacgcagcgg
0001 atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg
0071 cctttttggg gattttgctg ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa ttgttcgctt
0141 taccgactac cagacccgct ctaatgaaaa ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt
0211 atctacgatc gtaacggtat ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga
0281 aagtcgataa cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
0351 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa cctgaccgaa
0421 gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga agttaaaggc tataaacgtc
0491 gttactatcc ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg ataaagacgt
0561 cgaacgcctg aataatgacg gcaaactggc caactatgcg gcaacgcatg atatcggtaa gctgggcatt
0631 gagcgttact atgaagatgt gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc
0701 gtgttattcg ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
0771 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg tagtcaccga tccgcgtaca
0841 ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacttgtt tgttgacggt atctccagca
0911 aagattattc cgccttgttg aacgatccga atacaccgct ggtgaaccgc gccacacagg gggtttatcc
0981 tcccgcgtct acagttaaac cctatgtggc ggtttcggca ttgagcgccg gggtgatcac gcgcaatacg
1051 acgctgtttg acccaggctg gtggcaactg ccaggttcgg aaaaacgtta tcgtgactgg aaaaaatggg
1121 gccacgggcg tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
1191 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac cggtatcgac
1261 ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa acgctttaaa aaaccgtggt
1331 atcagggtga caccattccg gttggtatcg gtcagggtta ctggacagcg accccaatcc agatgagtaa
1401 ggcactgatg atcctgatta atgacggtat cgtgaaggtt cctcatttgc tgatgagcac cgccgaagac
1471 ggcaaacagg tgccatgggt acagccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc
1541 tggcgaaaga cggtatgtac ggtgttgcta accgccctaa cggtacggcg cataaatact ttgctagcgc
1611 accgtacaaa attgcggcga aatccggtac cgctcaggtc ttcggtctga aagcgaacga aacctataat
1681 gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt tgcgccatac aacaatccgc
1751 aagtggctgt cgccatgatt ctggagaacg gtggtgcggg tccggcggtt ggtacactga tgcgccagat
1821 cctcgaccac attatgctgg gtgataacaa caccgatctg cctgcggaaa atccagcggt tgccgcagcg
1891 gaggaccatt aatcatgacg gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac
1961 aatgctgctg atcttactgg cattgctggt ttacagcgcc ct
|
