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PEC Original Annotations
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Essentiality
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Class
|
non-essential
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References (PMID)
|
|
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Deletion
|
OCR02-6
(D)
,
DE(ilvE-ilvC)2049
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
ilvA
|
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Related strains
|
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Strains Search
|
Search strains by gene name:
ilvA
Search strains by all related name:
ilvA b3772 ECK3764 JW3745 o514
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General information (Go to Linear View: )
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Gene Name
|
ilvA
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Alternative name
|
b3772,ECK3764,JW3745,o514
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Location, Length
|
3,953,354 - 3,954,898
(
+
)
;
85.21
min
;
1545
(bp) ,
514
(aa)
|
|
Product
|
threonine deaminase
|
|
Operon Name
|
ilvLXG_1G_2MEDA
|
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Note
|
threonine deaminase (dehydratase)
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Function
|
enzyme; Amino acid biosynthesis: Isoleucine, Valine
|
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Gene Ontology
|
GO:0003824
;
catalytic activity ( ilvA )
GO:0004794
;
L-threonine ammonia-lyase activity ( ilvA )
GO:0006520
;
amino acid metabolic process ( ilvA )
GO:0008152
;
metabolic process ( ilvA )
GO:0008652
;
amino acid biosynthetic process ( ilvA )
GO:0009082
;
branched chain family amino acid biosynthetic process ( ilvA )
GO:0009097
;
isoleucine biosynthetic process ( ilvA )
GO:0016829
;
lyase activity ( ilvA )
GO:0030170
;
pyridoxal phosphate binding ( ilvA )
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PID
|
1790207
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EC number
(KEGG Pathway)
|
4.3.1.19
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
ilvA
(
EG10493
)
|
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Number of removed
|
0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
THD1_ECOLI
(
P04968
)
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- Protein name
|
Threonine dehydratase biosynthetic
|
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- Synonyms
|
EC 4.3.1.19, Threonine deaminase
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- Gene name
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Name=ilvA; OrderedLocusNames=b3772;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
ilvA
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
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PSI-BLAST
|
Chain , Threonine Deaminase
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|
SWISS-PROT
(database updated :
2007.02.20
)
|
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BLAST
|
Threonine dehydratase biosynthetic
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|
PSI-BLAST
|
Threonine dehydratase biosynthetic
|
|
nr
(database updated :
2007.02.20
)
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BLAST
|
threonine dehydratase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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|
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PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
PROKAR_LIPOPROTEIN
DEHYDRATASE_SER_THR
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MMBR References
|
J Bacteriol. 1985;162(2):598-606.
Physical analysis of deletion mutations in the ilvGEDA operon of Escherichia coli K-12.
Driver RP, Lawther RP.
(
2985538
)
|
J Bacteriol. 1982;149(3):1071-81.
Physical and genetic localization of ilv regulatory sites in lambda ilv bacteriophages.
Gray JE, Bennett DC, Umbarger HE, Calhoun DH.
(
6277848
)
|
J Bacteriol. 1992;174:4871-4877
Multiple transcripts encoded by the ilvGMEDA gene cluster of Escherichia coli K-12.
Huang, F., G. Coppola, and D. H. Calhoun.
|
Nucleic Acids Res. 1987;15:2137-2155
The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12.
Lawther, R. P., R. C. Wek, J. M. Lopes, R. Pereira, B. E. Taillon, and G. W. Hatfield.
|
J Biol Chem. 1989;264:1224-1231
Overlapping transcription and termination of the convergent ilvA and ilvY genes of Escherichia coli.
Sameshima, J. H., R. C. Wek, and G. W. Hatfield.
|
J Bacteriol. 1972;112(1):142-7.
Isoleucine and valine metabolism in Escherichia coli. XIX. Inhibition of isoleucine biosynthesis by glycyl-leucine.
Vonder Haar RA, Umbarger HE.
(
4562390
)
|
J Mol Biol. 1988;200(3):427-38.
Generation of a detailed physical and genetic map of the ilv-metE-udp region of the Escherichia coli chromosome.
Aldea M, Maples VF, Kushner SR.
(
3294419
)
|
Gene. 1987;56(2-3):185-98.
The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12.
Cox JL, Cox BJ, Fidanza V, Calhoun DH.
(
3315862
)
|
Science. 1992;257(5071):771-8.
Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes.
Daniels DL, Plunkett G 3rd, Burland VD, Blattner FR.
(
1379743
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MADSQPLSGA PEGAEYLRAV LRAPVYEAAQ VTPLQKMEKL SSRLDNVILV KREDRQPVHS FKLRGAYAMM
0071 AGLTEEQKAH GVITASAGNH AQGVAFSSAR LGVKALIVMP TATADIKVDA VRGFGGEVLL HGANFDEAKA
0141 KAIELSQQQG FTWVPPFDHP MVIAGQGTLA LELLQQDAHL DRVFVPVGGG GLAAGVAVLI KQLMPQIKVI
0211 AVEAEDSACL KAALDAGHPV DLPRVGLFAE GVAVKRIGDE TFRLCQEYLD DIITVDSDAI CAAMKDLFED
0281 VRAVAEPSGA LALAGMKKYI ALHNIRGERL AHILSGANVN FHGLRYVSER CELGEQREAL LAVTIPEEKG
0351 SFLKFCQLLG GRSVTEFNYR FADAKNACIF VGVRLSRGLE ERKEILQMLN DGGYSVVDLS DDEMAKLHVR
0421 YMVGGRPSHP LQERLYSFEF PESPGALLRF LNTLGTYWNI SLFHYRSHGT DYGRVLAAFE LGDHEPDFET
0491 RLNELGYDCH DETNNPAFRF FLAG
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GTGAACGTCA GGTCTCCTTT GCCCTGCGTG
-070 CTTATGCCAG CCTGGCAACC AGCGCCGACA AAGGCGCGGT GCGCGATAAA TCGAAACTGG GGGGTTAATA
0001 atggctgact cgcaacccct gtccggtgct ccggaaggtg ccgaatattt aagagcagtg ctgcgcgcgc
0071 cggtttacga ggcggcgcag gttacgccgc tacaaaaaat ggaaaaactg tcgtcgcgtc ttgataacgt
0141 cattctggtg aagcgcgaag atcgccagcc agtgcacagc tttaagctgc gcggcgcata cgccatgatg
0211 gcgggcctga cggaagaaca gaaagcgcac ggcgtgatca ctgcttctgc gggtaaccac gcgcagggcg
0281 tcgcgttttc ttctgcgcgg ttaggcgtga aggccctgat cgttatgcca accgccaccg ccgacatcaa
0351 agtcgacgcg gtgcgcggct tcggcggcga agtgctgctc cacggcgcga actttgatga agcgaaagcc
0421 aaagcgatcg aactgtcaca gcagcagggg ttcacctggg tgccgccgtt cgaccatccg atggtgattg
0491 ccgggcaagg cacgctggcg ctggaactgc tccagcagga cgcccatctc gaccgcgtat ttgtgccagt
0561 cggcggcggc ggtctggctg ctggcgtggc ggtgctgatc aaacaactga tgccgcaaat caaagtgatc
0631 gccgtagaag cggaagactc cgcctgcctg aaagcagcgc tggatgcggg tcatccggtt gatctgccgc
0701 gcgtagggct atttgctgaa ggcgtagcgg taaaacgcat cggtgacgaa accttccgtt tatgccagga
0771 gtatctcgac gacatcatca ccgtcgatag cgatgcgatc tgtgcggcga tgaaggattt attcgaagat
0841 gtgcgcgcgg tggcggaacc ctctggcgcg ctggcgctgg cgggaatgaa aaaatatatc gccctgcaca
0911 acattcgcgg cgaacggctg gcgcatattc tttccggtgc caacgtgaac ttccacggcc tgcgctacgt
0981 ctcagaacgc tgcgaactgg gcgaacagcg tgaagcgttg ttggcggtga ccattccgga agaaaaaggc
1051 agcttcctca aattctgcca actgcttggc gggcgttcgg tcaccgagtt caactaccgt tttgccgatg
1121 ccaaaaacgc ctgcatcttt gtcggtgtgc gcctgagccg cggcctcgaa gagcgcaaag aaattttgca
1191 gatgctcaac gacggcggct acagcgtggt tgatctctcc gacgacgaaa tggcgaagct acacgtgcgc
1261 tatatggtcg gcggacgtcc atcgcatccg ttgcaggaac gcctctacag cttcgaattc ccggaatcac
1331 cgggcgcgct gctgcgcttc ctcaacacgc tgggtacgta ctggaacatt tctttgttcc actatcgcag
1401 ccatggcacc gactacgggc gcgtactggc ggcgttcgaa cttggcgacc atgaaccgga tttcgaaacc
1471 cggctgaatg agctgggcta cgattgccac gacgaaacca ataacccggc gttcaggttc tttttggcgg
1541 gttagGGAAA AATGCCTGAT AGCGCTTCGC TTATCAGGCC TACCCGCGCG ACAACGTCAT TTGTGGTTCG
1611 GCAAAATCTT CCAGAATGCC TCAATTAGCG GCTCA
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