MG1655
W3110
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Gene Report : ddlA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR38 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  ddlA
Related strains
     Strains Search Search strains by gene name:  ddlA   Search strains by all related name:  ddlA b0381 ECK0376 JW0372

General information  (Go to Linear View:)
 Gene Name ddlA  
 Alternative name b0381,ECK0376,JW0372  
 Location, Length 399,053 - 400,147 (  -  ) ;   8.60 min ; 1095 (bp) ,   364 (aa) Go to Linear View
 Product D-alanine-D-alanine ligase A  
 Operon Name ddlA  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009252 - peptidoglycan biosynthetic process  
 Function enzyme; Murein sacculus, peptidoglycan  
 Gene Ontology GO:0000166 ; nucleotide binding ( ddlA )
GO:0003824 ; catalytic activity ( ddlA )
GO:0005524 ; ATP binding ( ddlA )
GO:0005618 ; cell wall ( ddlA )
GO:0005737 ; cytoplasm ( ddlA )
GO:0007047 ; cell wall organization and biogenesis ( ddlA )
GO:0008360 ; regulation of cell shape ( ddlA )
GO:0008716 ; D-alanine-D-alanine ligase activity ( ddlA )
GO:0009252 ; peptidoglycan biosynthetic process ( ddlA )
GO:0016874 ; ligase activity ( ddlA )
GO:0030145 ; manganese ion binding ( ddlA )
GO:0046872 ; metal ion binding ( ddlA )
 PID 1786579  
 EC number
  (KEGG Pathway)   		6.3.2.4  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) DDLA_ECOLI ( P23844 )
    -  Protein name D-alanine--D-alanine ligase A  
    -  Synonyms EC 6.3.2.4, D-alanylalanine synthetase A, D-Ala-D-Ala ligase A  
    -  Gene name Name=ddlA; OrderedLocusNames=b0381, z0477, ECs0431, SF0232, S0254;  

 Linear View (Whole Mode)
View Location
   390.0  –  415.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP ddlA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Allosteric Inhibition Of Staphylococcus Aureus D-Alanine:d- Alanine Ligase Revealed By Crystallographic Studies  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST D-alanine--D-alanine ligase A (D-alanylalanine synthetase A) (D-Ala-D-Ala ligase A)  
         PSI-BLAST D-alanine--D-alanine ligase A
    nr     (database updated : 2007.02.20 )
         BLAST D-alanylalanine synthetase [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    ATP_GTP_A    DALA_DALA_LIGASE_1    DALA_DALA_LIGASE_2     

Other Cross-Reference
    COG COG1181M 
    EcoCyc ddlA 

MMBR References
Biochemistry. 1996;35(32):10464-71.
Gain of D-alanyl-D-lactate or D-lactyl-D-alanine synthetase activities in three active-site mutants of the Escherichia coli D-alanyl-D-alanine ligase B.
Park IS, Lin CH, Walsh CT. ( 8756703 )
Biochemistry. 1991;30(6):1673-82.
Existence of two D-alanine:D-alanine ligases in Escherichia coli: cloning and sequencing of the ddlA gene and purification and characterization of the DdlA and DdlB enzymes.
Zawadzke LE, Bugg TD, Walsh CT. ( 1993184 )

Sequences
Amino acid
FASTA format 
0001 MEKLRVGIVF GGKSAEHEVS LQSAKNIVDA IDKSRFDVVL LGIDKQGQWH VSDASNYLLN ADDPAHIALR 
0071 PSATSLAQVP GKHEHQLIDA QNGQPLPTVD VIFPIVHGTL GEDGSLQGML RVANLPFVGS DVLASAACMD 
0141 KDVTKRLLRD AGLNIAPFIT LTRANRHNIS FAEVESKLGL PLFVKPANQG SSVGVSKVTS EEQYAIAVDL 
0211 AFEFDHKVIV EQGIKGREIE CAVLGNDNPQ ASTCGEIVLT SDFYAYDTKY IDEDGAKVVV PAAIAPEIND 
0281 KIRAIAVQAY QTLGCAGMAR VDVFLTPENE VVINEINTLP GFTNISMYPK LWQASGLGYT DLITRLIELA 
0351 LERHAADNAL KTTM
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             ttgtcagcag aagattgcaa caatctttca 
-070 gaaggccgcg aatcccccgt tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag 
0001 atggaaaaac tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg 
0071 caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg ataaacaagg 
0141 gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc ctgcccatat tgcgttgcgc 
0211 ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg agcatcagct tatcgacgcg caaaacggtc 
0281 agccgttgcc gacggtggat gtcattttcc cgattgtcca cggtacgctg ggcgaagatg gttccttgca 
0351 gggaatgctg cgggtcgcca atttaccgtt tgtaggttct gatgttctgg cttcagcagc ctgtatggac 
0421 aaagatgtca ccaaacgtct gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg 
0491 ccaatcgtca caacattagt tttgccgaag tggagtctaa actggggtta ccactgtttg taaaaccggc 
0561 taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt acgcaattgc cgtcgatctg 
0631 gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga tcaaaggtcg tgagatcgaa tgcgcagttc 
0701 tgggcaacga caatccgcaa gccagcacct gtggagagat cgtactcacc agcgatttct atgcctacga 
0771 caccaagtac attgacgaag atggcgcgaa agtggtagtt ccggcagcca ttgcgccaga aatcaacgat 
0841 aagatccggg cgattgccgt tcaggcttat caaacgttgg gatgcgcagg catggcgcgt gtagacgtgt 
0911 ttttaacccc agagaacgaa gtggtgatca acgagatcaa caccctgcct ggcttcacta atatcagtat 
0981 gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca cacgtttgat tgaactggcg 
1051 ctggagcgtc acgctgcgga taacgcattg aaaaccacaa tgtaatattt taacgcccga taatattctt 
1121 attgggcgtt ttcagtttct tcatcgcgac gacgaataat caaccccgcc agccagaaac taatcaccca 
1191 cgtca