MG1655
W3110
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tmRNA Report : ssrA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Proc Natl Acad Sci U S A. 1994;91(20):9223-7.
A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli.
Komine Y, Kitabatake M, Yokogawa T, Nishikawa K, Inokuchi H. ( 7524073 )
     Deletion OCL51 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  ssrA
Related strains
     Strains Search Search strains by gene name:  ssrA   Search strains by all related name:  ssrA b2621 ECK2617 JWR0055 sipB

General information  (Go to Linear View:)
 tmRNA Name ssrA  
 Alternative name b2621,ECK2617,JWR0055,sipB  
 Location, Length 2,753,615 - 2,753,977 (  +  ) ;   59.35 min ; 363 (bp) , Go to Linear View
 Product tmRNA, 10Sa RNA  
 Operon Name ssrA  
 Note acts as tRNA-Ala and mRNA template for tagging proteins resulting from premature transcription termination for degradation, a process known as trans-translation  
 Function 2.2.6 information transfer; RNA related; rRNA, stable RNA,2.3.6 information transfer; protein related; turnover, degradation,6.6 cell structure; ribosome,7.1 location of gene products; cytoplasm,RNA; Ribosomal and stable RNAs  
 Gene Ontology  
 PID  
 EC number
  (KEGG Pathway)
 
 

 Linear View (Whole Mode)
View Location
   2745.0  –  2770.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP  
    PDB     (database updated : 2007.02.20 )
        PSI-BLAST  
    SWISS-PROT     (database updated : 2007.02.20 )
        BLAST  
        PSI-BLAST
    nr     (database updated : 2007.02.20 )
        BLAST  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE  

Other Cross-Reference
    EcoCyc ssrA 

MMBR References
Genes Genet Syst. 1996;71(1):47-50.
10Sa RNA complements the temperature-sensitive phenotype caused by a mutation in the phosphoribosyl pyrophosphate synthetase (prs) gene in Escherichia coli.
Ando H, Kitabatake M, Inokuchi H. ( 8925474 )
Biochimie. 1996;78(11-12):979-83.
Structural organization of Escherichia coli tmRNA.
Felden B, Himeno H, Muto A, Atkins JF, Gesteland RF. ( 9150875 )
J Bacteriol. 1991;173(17):5252
Physical map locations of the genes that encode small stable RNAs in Escherichia coli.
Komine Y, Inokuchi H. ( 1715853 )
J. Biochem. (Tokyo) 1996;119:463-467
10Sa RNA is associated with 70S ribosome particles in Escherichia coli.
Komine, Y., M. Kitabatake, and H. Inokuchi.
Biochimie. 1996;78(11-12):985-91.
Structure and function of 10Sa RNA: trans-translation system.
Muto A, Sato M, Tadaki T, Fukushima M, Ushida C, Himeno H. ( 9150876 )
J Bacteriol. 1990;172(8):4708-9.
Location of a gene (ssrA) for a small, stable RNA (10Sa RNA) in the Escherichia coli chromosome.
Oh BK, Chauhan AK, Isono K, Apirion D. ( 1695897 )
Mol Gen Genet. 1991;229(1):52-6.
10Sa RNA, a small stable RNA of Escherichia coli, is functional.
Oh BK, Apirion D. ( 1716727 )
Cell. 1995;83(2):227-35.
A role for a small stable RNA in modulating the activity of DNA-binding proteins.
Retallack DM, Friedman DI. ( 7585940 )
J Bacteriol. 1994;176:2082-2089
Role for 10Sa RNA in the growth of lambda-P22 hybrid phage.
Retallack, D. M., L. L. Johnson, and D. I. Friedman.
Mol Microbiol. 1989;3(11):1481-5.
The gene for a small stable RNA (10Sa RNA) of Escherichia coli.
Chauhan AK, Apirion D. ( 2482406 )

Sequences
Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CGAATAAAAA TCAGGCTACA TGGGTGCTAA 
-070 ATCTTTAACG ATAACGCCAT TGAGGCTGGT CATGGCGCTC ATAAATCTGG TATACTTACC TTTACACATT 
0001 ggggctgatt ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa 
0071 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc ttagagccct 
0141 ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca aaagagatcg cgtggaagcc 
0211 ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt tgttagtggc gtgtccgtcc gcagctggca 
0281 agcgaatgta aagactgact aagcatgtag taccgaggat gtaggaattt cggacgcggg ttcaactccc 
0351 gccagctcca ccaAAATTCT CCATCGGTGA TTACCAGAGT CATCCGATGA AGTCCTAAGA GCCCGCACGG 
0421 CGCAAGCCCT GCGGGCTTTT TTGTGCCCTC AATTTGTCCC GCG