MG1655
W3110
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Gene Report : pyrD
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR55 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  pyrD
Related strains
     Strains Search Search strains by gene name:  pyrD   Search strains by all related name:  pyrD b0945 ECK0936 JW0928

General information  (Go to Linear View:)
 Gene Name pyrD  
 Alternative name b0945,ECK0936,JW0928  
 Location, Length 1,003,991 - 1,005,001 (  +  ) ;   21.64 min ; 1011 (bp) ,   336 (aa) Go to Linear View
 Product dihydro-orotate oxidase, FMN-linked  
 Operon Name pyrD  
 Note dihydro-orotate dehydrogenase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006221 - pyrimidine nucleotide biosynthetic process  
 Function enzyme; Pyrimidine ribonucleotide biosynthesis  
 Gene Ontology GO:0003824 ; catalytic activity ( pyrD )
GO:0004152 ; dihydroorotate dehydrogenase activity ( pyrD )
GO:0004158 ; dihydroorotate oxidase activity ( pyrD )
GO:0005886 ; plasma membrane ( pyrD )
GO:0006207 ; 'de novo' pyrimidine base biosynthetic process ( pyrD )
GO:0006221 ; pyrimidine nucleotide biosynthetic process ( pyrD )
GO:0006222 ; UMP biosynthetic process ( pyrD )
GO:0008152 ; metabolic process ( pyrD )
GO:0016020 ; membrane ( pyrD )
GO:0016491 ; oxidoreductase activity ( pyrD )
 PID 1787177  
 EC number
  (KEGG Pathway)   		1.3.5.2  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene pyrD ( EG10807 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) PYRD_ECOLI ( P05021 )
    -  Protein name Dihydroorotate dehydrogenase  
    -  Synonyms EC 1.3.3.1, Dihydroorotate oxidase, DHOdehase, DHODase, DHOD  
    -  Gene name Name=pyrD; OrderedLocusNames=b0945, z1294, ECs1029;  

 Linear View (Whole Mode)
View Location
   995.0  –  1020.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP pyrD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Escherichia Coli Dihydroorotate Dehydrogenase  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Dihydroorotate dehydrogenase (Dihydroorotate oxidase) (DHOdehase) (DHODase) (DHOD)  
         PSI-BLAST Dihydroorotate dehydrogenase (Dihydroorotate oxidase) (DHOdehase) (DHODase) (DHOD)
    nr     (database updated : 2007.02.20 )
         BLAST dihydroorotate dehydrogenase [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    MICROBODIES_CTER    DHODEHASE_1    DHODEHASE_2     

Other Cross-Reference
    COG COG0167F 
    EcoCyc pyrD 

MMBR References
Eur J Biochem. 1984;140:343-352
Studies on the structure and expression of Escherichia coli pyrC, pyrD and pyrF using the cloned genes.
Jensen, K. F., J. N. Larsen, L. Schack, and A. Sivertsen.
Eur J Biochem. 1985;151:59-65
Nucleotide sequence of the pyrD gene of Escherichia coli and characterization of the flavoprotein dihydroorotate dehydrogenase.
Larsen, J. N., and K. F. Jensen.

Sequences
Amino acid
FASTA format 
0001 MYYPFVRKAL FQLDPERAHE FTFQQLRRIT GTPFEALVRQ KVPAKPVNCM GLTFKNPLGL AAGLDKDGEC 
0071 IDALGAMGFG SIEIGTVTPR PQPGNDKPRL FRLVDAEGLI NRMGFNNLGV DNLVENVKKA HYDGVLGINI 
0141 GKNKDTPVEQ GKDDYLICME KIYAYAGYIA INISSPNTPG LRTLQYGEAL DDLLTAIKNK QNDLQAMHHK 
0211 YVPIAVKIAP DLSEEELIQV ADSLVRHNID GVIATNTTLD RSLVQGMKNC DQTGGLSGRP LQLKSTEIIR 
0281 RLSLELNGRL PIIGVGGIDS VIAAREKIAA GASLVQIYSG FIFKGPPLIK EIVTHI
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             GAAAACGTTT GCGTTTTTTT TGCCGCAGGT 
-070 CAATTCCCTT TTGGTCCGAA CTCGCACATA ATACGCCCCC GGTTTGCACA CCGGGAATCC AGGAGAGTTC 
0001 atgtactacc ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc 
0071 agcaattacg ccgtattaca ggaacgccgt ttgaagcact ggtgcggcag aaagtgcctg cgaaacctgt 
0141 taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc ttgataaaga cggggagtgc 
0211 attgacgcgt taggcgcgat gggatttgga tcgatcgaga tcggtaccgt cacgccacgt ccacagccag 
0281 gtaatgacaa gccgcgtctc tttcgtctgg tagatgccga aggtttgatc aaccgtatgg gctttaataa 
0351 tcttggcgtt gataacctcg tagagaacgt aaaaaaggcc cattatgacg gcgtcctggg tattaacatc 
0421 ggcaaaaata aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg 
0491 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc tgcaatatgg 
0561 tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt tgcaagcgat gcaccataaa 
0631 tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg aagaagaatt gatccaggtt gccgatagtt 
0701 tagttcgcca taatattgat ggcgttattg caaccaatac cacactcgat cgttctcttg ttcagggaat 
0771 gaaaaattgc gatcaaaccg gtggcttaag tggtcgtccg cttcagttaa aaagcaccga aattattcgc 
0841 cgcttgtcac tggaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg 
0911 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta aaggtccgcc 
0981 gctgattaaa gaaatcgtta cccatatcta aTTATTTCTT CGAATTCCTC TTTATAAGAT AATCAGGGCT 
1051 TTATTTTCAG CCCTGGTTGT TTTATATTCA TCGCTGTTGC TTATTTAGAC ATTTTGTACT T