MG1655
W3110
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Gene Report : lolA
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
FEBS Lett. 1998;439(1-2):51-4.
Genetic analyses of the in vivo function of LolA, a periplasmic chaperone involved in the outer membrane localization of Escherichia coli lipoproteins.
Tajima T, Yokota N, Matsuyama S, Tokuda H. ( 9849875 )
     Deletion OCR51 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  lolA
Related strains
     Strains Search Search strains by gene name:  lolA   Search strains by all related name:  lolA b0891 ECK0882 JW0874 lplA yzzV

General information  (Go to Linear View:)
 Gene Name lolA  
 Alternative name b0891,ECK0882,JW0874,lplA,yzzV  
 Location, Length 936,595 - 937,206 (  +  ) ;   20.19 min ; 612 (bp) ,   203 (aa) Go to Linear View
 Product lipoprotein chaperone  
 Operon Name lolA-rarA  
 Note periplasmic protein effects translocation of lipoproteins from inner membrane to outer; GO_component: GO:0042597 - periplasmic space  
 Function transport; Protein, peptide secretion  
 Gene Ontology GO:0000166; nucleotide binding ( lplA )
GO:0003824; catalytic activity ( lplA )
GO:0005515; protein binding ( lolA )
GO:0005515; protein binding ( lplA )
GO:0005524; ATP binding ( lplA )
GO:0005737; cytoplasm ( lplA )
GO:0006464; protein modification process ( lplA )
GO:0006810; transport ( lolA )
GO:0008565; protein transporter activity ( lolA )
GO:0015031; protein transport ( lolA )
GO:0016740; transferase activity ( lplA )
GO:0016979; lipoate-protein ligase activity ( lplA )
GO:0018055; peptidyl-lysine lipoylation ( lplA )
GO:0030288; outer membrane-bounded periplasmic space ( lolA )
GO:0042597; periplasmic space ( lolA )
 PID 145693108  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene lolA ( EG12684 )  
    Number of removed 22 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) LOLA_ECOLI ( P61316 ; P39178; Q8X5H8 )
    -  Protein name Outer-membrane lipoprotein carrier protein precursor  
    -  Synonyms P20  
    -  Gene name Name=lolA; Synonyms=lplA; OrderedLocusNames=b0891;  

 Linear View (Whole Mode)
View Location
   925.0  –  950.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP lolA (  homologous genes of other bacterias  )   lplA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of The Lipoprotein Localization Factor, Lola  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Outer-membrane lipoprotein carrier protein precursor (P20)  
         PSI-BLAST Outer-membrane lipoprotein carrier protein precursor (P20)
    nr     (database updated : 2007.02.20 )
         BLAST outer-membrane lipoprotein carrier protein precursor [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG2834M 
    EcoCyc lolA 

MMBR References
EMBO J. 1997;16:6947-6955
A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli.
Matsuyama, S., N. Yokota, and H. Tokuda.
EMBO J. 1995;14:3365-3372
A novel periplasmic carrier protein involved in the sorting and transport of Escherichia coli lipoproteins destined for the outer membrane.
Matsuyama, S., T. Tajima, and H. Tokuda.

Sequences
Amino acid
FASTA format
0001 MKKIAITCAL LSSLVASSVW ADAASDLKSR LDKVSSFHAS FTQKVTDGSG AAVQEGQGDL WVKRPNLFNW 
0071 HMTQPDESIL VSDGKTLWFY NPFVEQATAT WLKDATGNTP FMLIARNQSS DWQQYNIKQN GDDFVLTPKA 
0141 SNGNLKQFTI NVGRDGTIHQ FSAVEQDDQR SSYQLKSQQN GAVDAAKFTF TPPQGVTVDD QRK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             GAAAATTCAG TATTTTCTTC TTTCCTCAAG 
-070 CTGATTATTA GCCTGGAATA GAGAGTAGAG GGAACTCCCG ATCGGGAGTG ACGTAATTTG AGGAATAATG 
0001 atgaaaaaaa ttgccatcac ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg gctgatgccg 
0071 caagcgatct gaaaagccgc ctggataaag tcagcagctt ccacgccagc ttcacacaaa aagtgactga 
0141 cggtagcggc gcggcggtgc aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg 
0211 catatgacac aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg 
0281 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa 
0351 ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac gccgaaagcc 
0421 agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac aatccatcag tttagcgcgg 
0491 tggagcagga cgatcagcgc agcagttatc aactgaaatc ccagcaaaat ggggctgtgg atgcagcgaa 
0561 atttaccttc accccgccgc aaggcgtcac ggtagatgat caacgtaagt agAGGCACCT GAGTGAGCAA 
0631 TCTGTCGCTC GATTTTTCGG ATAATACTTT TCAACCTCTG GCCGCGCGTA TGCGGCCAGA AAATTTAGCA 
0701 CAGTATATCG GC