MG1655
W3110
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Gene Report : murF
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Biochemistry. 1997;36(20):6223-9.
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS. ( 9166795 )
     Deletion OCR30,31-3 (BK2-1 / AK1-1) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  murF
Related strains
     Strains Search Search strains by gene name:  murF   Search strains by all related name:  murF b0086 ECK0087 JW0084 mra

General information  (Go to Linear View:)
 Gene Name murF  
 Alternative name b0086,ECK0087,JW0084,mra  
 Location, Length 94,650 - 96,008 (  +  ) ;   2.04 min ; 1359 (bp) ,   452 (aa) Go to Linear View
 Product UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase  
 Operon Name mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0000270 - peptidoglycan metabolic process  
 Function enzyme; Murein sacculus, peptidoglycan,1.6.7 metabolism; macromolecules (cellular constituent) biosynthesis; peptidoglycan (murein),1.7.34 metabolism; central intermediary metabolism; peptidoglycan (murein),1.7.34 metabolism; central intermediary metaboli  
 Gene Ontology GO:0000166; nucleotide binding ( murF )
GO:0005524; ATP binding ( murF )
GO:0005737; cytoplasm ( murF )
GO:0007047; cell wall organization and biogenesis ( murF )
GO:0007049; cell cycle ( murF )
GO:0008360; regulation of cell shape ( murF )
GO:0008766; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ( murF )
GO:0009058; biosynthetic process ( murF )
GO:0009252; peptidoglycan biosynthetic process ( murF )
GO:0009273; peptidoglycan-based cell wall biogenesis ( murF )
GO:0016874; ligase activity ( murF )
GO:0047480; UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity ( murF )
GO:0051301; cell division ( murF )
 PID 1786274  
 EC number
  (KEGG Pathway)
 
6.3.2.10  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene murF ( EG10622 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) MURF_ECOLI ( P11880 ; O07100; P77636 )
    -  Protein name UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase  
    -  Synonyms EC 6.3.2.10, UDP-MurNAc-pentapeptide synthetase, D-alanyl-D- alanine-adding enzyme  
    -  Gene name Name=murF; Synonyms=mra; OrderedLocusNames=b0086;  

 Linear View (Whole Mode)
View Location
   85.0  –  110.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP murF (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Escherichia Coli Udpmurnac-Tripeptide D-Alanyl-D-Alanine-Adding Enzyme (Murf) At 2.3 Angstrom Resolution  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase  
         PSI-BLAST UDP-N-acetylmuramate--L-alanine ligase
    nr     (database updated : 2007.02.20 )
         BLAST UDP-MurNAc-Tripeptide:D-Ala-D-Ala-Adding Enzyme [Escherichia coli]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AA_TRNA_LIGASE_II_2     

Other Cross-Reference
    COG COG0770M 
    EcoCyc murF 

MMBR References
Biochemistry. 1996;35(50):16264-9.
Kinetic mechanism of the Escherichia coli UDPMurNAc-tripeptide D-alanyl-D-alanine-adding enzyme: use of a glutathione S-transferase fusion.
Anderson MS, Eveland SS, Onishi HR, Pompliano DL. ( 8973200 )
Biochemistry. 1997;36(20):6223-9.
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS. ( 9166795 )
J Bacteriol. 1989;171(11):6379-82.
Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes.
Jung HK, Ishino F, Matsuhashi M. ( 2553679 )
J Bacteriol. 1989;171(11):6126-34.
Organization of the murE-murG region of Escherichia coli: identification of the murD gene encoding the D-glutamic-acid-adding enzyme.
Mengin-Lecreulx D, Parquet C, Desviat LR, Pla J, Flouret B, Ayala JA, van Heijenoort J. ( 2681153 )
Nucleic Acids Res. 1989;17(13):5379
Nucleotide sequence of the murF gene encoding the UDP-MurNAc-pentapeptide synthetase of Escherichia coli.
Parquet C, Flouret B, Mengin-Lecreulx D, van Heijenoort J. ( 2668880 )

Sequences
Amino acid
FASTA format
0001 MISVTLSQLT DILNGELQGA DITLDAVTTD TRKLTPGCLF VALKGERFDA HDFADQAKAG GAGALLVSRP 
0071 LDIDLPQLIV KDTRLAFGEL AAWVRQQVPA RVVALTGSSG KTSVKEMTAA ILSQCGNTLY TAGNLNNDIG 
0141 VPMTLLRLTP EYDYAVIELG ANHQGEIAWT VSLTRPEAAL VNNLAAAHLE GFGSLAGVAK AKGEIFSGLP 
0211 ENGIAIMNAD NNDWLNWQSV IGSRKVWRFS PNAANSDFTA TNIHVTSHGT EFTLQTPTGS VDVLLPLPGR 
0281 HNIANALAAA ALSMSVGATL DAIKAGLANL KAVPGRLFPI QLAENQLLLD DSYNANVGSM TAAVQVLAEM 
0351 PGYRVLVVGD MAELGAESEA CHVQVGEAAK AAGIDRVLSV GKQSHAISTA SGVGEHFADK TALITRLKLL 
0421 IAEQQVITIL VKGSRSAAME EVVRALQENG TC

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             TGGTCGCGGG CAAAGGCCAT GAAGATTACC 
-070 AGATTGTTGG CAATCAGCGT CTGGACTACT CCGATCGCGT CACGGTGGCG CGTCTGCTGG GGGTGATTGC 
0001 atgattagcg taacccttag ccaacttacc gacattctca acggtgaact gcaaggtgca gatatcaccc 
0071 ttgatgctgt aaccactgat acccgaaaac tgacgccggg ctgcctgttt gttgccctga aaggcgaacg 
0141 ttttgatgcc cacgattttg ccgaccaggc gaaagctggc ggcgcaggcg cactactggt tagccgtccg 
0211 ctggacatcg acctgccgca gttaatcgtc aaggatacgc gtctggcgtt tggtgaactg gctgcatggg 
0281 ttcgccagca agttccggcg cgcgtggttg ctctgacggg gtcctccggc aaaacctccg ttaaagagat 
0351 gacggcggcg attttaagcc agtgcggcaa cacgctttat acggcaggca atctcaacaa cgacatcggt 
0421 gtaccgatga cgctgttgcg cttaacgccg gaatacgatt acgcagttat tgaacttggc gcgaaccatc 
0491 agggcgaaat agcctggact gtgagtctga ctcgcccgga agctgcgctg gtcaacaacc tggcagcggc 
0561 gcatctggaa ggttttggct cgcttgcggg tgtcgcgaaa gcgaaaggtg aaatctttag cggcctgccg 
0631 gaaaacggta tcgccattat gaacgccgac aacaacgact ggctgaactg gcagagcgta attggctcac 
0701 gcaaagtgtg gcgtttctca cccaatgccg ccaacagcga tttcaccgcc accaatatcc atgtgacctc 
0771 gcacggtacg gaatttaccc tacaaacccc aaccggtagc gtcgatgttc tgctgccgtt gccggggcgt 
0841 cacaatattg cgaatgcgct ggcagccgct gcgctctcca tgtccgtggg cgcaacgctt gatgctatca 
0911 aagcggggct ggcaaatctg aaagctgttc caggccgtct gttccccatc caactggcag aaaaccagtt 
0981 gctgctcgac gactcctaca acgccaatgt cggttcaatg actgcagcag tccaggtact ggctgaaatg 
1051 ccgggctacc gcgtgctggt ggtgggcgat atggcggaac tgggcgctga aagcgaagcc tgccatgtac 
1121 aggtgggcga ggcggcaaaa gctgctggta ttgaccgcgt gttaagcgtg ggtaaacaaa gccatgctat 
1191 cagcaccgcc agcggcgttg gcgaacattt tgctgataaa actgcgttaa ttacgcgtct taaattactg 
1261 attgctgagc aacaggtaat tacgatttta gttaagggtt cacgtagtgc cgccatggaa gaggtagtac 
1331 gcgctttaca ggagaatggg acatgttagT TTGGCTGGCC GAACATTTGG TCAAATATTA TTCCGGCTTT 
1401 AACGTCTTTT CCTATCTGAC GTTTCGCGCC ATCGTCAGCC TGCTGACCGC GCTGTTCAT