MG1655
W3110
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Gene Report : moaA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Proc Natl Acad Sci U S A. 1990;87(1):360-3.
Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K-12.
Lejeune P, Danchin A. ( 2404280 )
     Deletion 15 (LD)  ,  DE(pgl-grxA)502  ,  15+dps (LD)  ,  OCR48,49-5 (D)  ,  OCR48,50-8 (LD 15D::Cm) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  moaA
Related strains
     Strains Search Search strains by gene name:  moaA   Search strains by all related name:  moaA b0781 bisA chlA ECK0770 JW0764 narA

General information  (Go to Linear View:)
 Gene Name moaA  
 Alternative name b0781,bisA,chlA,ECK0770,JW0764,narA  
 Location, Length 816,267 - 817,256 (  +  ) ;   17.59 min ; 990 (bp) ,   329 (aa) Go to Linear View
 Product molybdopterin biosynthesis protein A  
 Operon Name moaABCDE  
 Note molybdopterin biosynthesis, protein A; GO_process: GO:0006777 - Mo-molybdopterin cofactor biosynthetic process  
 Function enzyme; Biosynthesis of cofactors, carriers: Molybdopterin  
 Gene Ontology GO:0000166; nucleotide binding ( moaA )
GO:0003824; catalytic activity ( moaA )
GO:0005506; iron ion binding ( moaA )
GO:0005525; GTP binding ( moaA )
GO:0006777; Mo-molybdopterin cofactor biosynthetic process ( moaA )
GO:0008152; metabolic process ( moaA )
GO:0019008; molybdopterin synthase complex ( moaA )
GO:0046872; metal ion binding ( moaA )
GO:0051536; iron-sulfur cluster binding ( moaA )
GO:0051539; 4 iron, 4 sulfur cluster binding ( moaA )
 PID 1786999  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene moaA ( EG11595 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) MOAA_ECOLI ( P30745 )
    -  Protein name Molybdenum cofactor biosynthesis protein A.  
    -  Synonyms  
    -  Gene name Name=moaA; Synonyms=chlA1, chlA, narA, bisA; OrderedLocusNames=b0781;  

 Linear View (Whole Mode)
View Location
   805.0  –  830.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP moaA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Structure Of The S-Adenosylmethionine Dependent Enzyme Moaa  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Molybdenum cofactor biosynthesis protein A  
         PSI-BLAST Molybdenum cofactor biosynthesis protein A
    nr     (database updated : 2007.02.20 )
         BLAST molybdenum cofactor biosynthesis protein A [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    MOAA_NIFB_PQQE     

Other Cross-Reference
    COG COG2896H 
    EcoCyc moaA 

MMBR References
J Bacteriol. 1982;149(2):469-78.
Molybdenum cofactor requirement for biotin sulfoxide reduction in Escherichia coli.
del Campillo-Campbell A, Campbell A. ( 6460021 )
J Bacteriol. 1979;138(3):726-30.
Use of chlC-lac fusions to determine regulation of gene chlC in Escherichia coli K-12.
Fimmel AL, Haddock BA. ( 110766 )
Mol Gen Genet. 1972;116(1):1-10.
Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures.
Glaser JH, DeMoss JA. ( 4558444 )
Proc Natl Acad Sci U S A. 1968;61(3):956-62.
A deletion analysis of prophage lambda and adjacent genetic regions.
Adhya S, Cleary P, Campbell A. ( 5246560 )
Microbiol. Rev. 1983;47:180-230.
Linkage map of Escherichia coli K-12, edition 7.
Bachmann, B. J.
J Bacteriol. 1987;169(1):117-25.
Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis.
Johnson ME, Rajagopalan KV. ( 2947896 )
Mol Microbiol. 1991;5(4):901-7.
Regulation of the chlA locus of Escherichia coli K12: involvement of molybdenum cofactor.
Baker KP, Boxer DH. ( 1906967 )
J Gen Microbiol. 1976;93(1):173-6.
A novel method for isolating chlorate-resistant mutants of Escherichia coli K12 by anaerobic selection on a lactate plus fumarate medium.
Lambdren PR, Guest JR. ( 772165 )
Mol Gen Genet. 1987;206(2):352-5.
Cloning of seven differently complementing DNA fragments with chl functions from Escherichia coli K12.
Reiss J, Kleinhofs A, Klingmuller W. ( 3035339 )
Mol Microbiol. 1992;6(22):3452-4.
Proposed nomenclature for the genes involved in molybdenum metabolism in Escherichia coli and Salmonella typhimurium.
Shanmugam KT, Stewart V, Gunsalus RP, Boxer DH, Cole JA, Chippaux M, DeMoss JA, Giordano G, Lin EC, Rajagopalan KV. ( 1484496 )
Mol Gen Genet. 1968;103(2):127-40.
Transduction of nitrate reductase loci of Escherichia coli by phages P-1 and lambda.
Venables WA, Guest JR. ( 4890160 )
J Bacteriol. 1975;121(1):259-66.
Positive selection of mutants with deletions of the gal-chl region of the Salmonella chromosome as a screening procedure for mutagens that cause deletions.
Alper MD, Ames BN. ( 1090571 )

Sequences
Amino acid
FASTA format
0001 MASQLTDAFA RKFYYLRLSI TDVCNFRCTY CLPDGYKPSG VTNKGFLTVD EIRRVTRAFA RLGTEKVRLT 
0071 GGEPSLRRDF TDIIAAVREN DAIRQIAVTT NGYRLERDVA SWRDAGLTGI NVSVDSLDAR QFHAITGQDK 
0141 FNQVMAGIDA AFEAGFEKVK VNTVLMRDVN HHQLDTFLNW IQHRPIQLRF IELMETGEGS ELFRKHHISG 
0211 QVLRDELLRR GWIHQLRQRS DGPAQVFCHP DYAGEIGLIM PYEKDFCATC NRLRVSSIGK LHLCLFGEGG 
0281 VNLRDLLEDD TQQQALEARI SAALREKKQT HFLHQNNTGI TQNLSYIGG

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             GGTCAACTGA TACGGTGCTT TGGCCGTGAC 
-070 AATGCTCGTA AAGATTGCCA CCAGGGCGAA GGAAGAAATG ACTTCGCCTC CCGTATCTGG AAAGGTGTAC 
0001 atggcttcac aactgactga tgcatttgcg cgtaagtttt actacttgcg cctgtcgatt accgatgtgt 
0071 gtaactttcg ttgcacctac tgcctgccgg atggctacaa accgagcggc gtcaccaata aaggctttct 
0141 taccgtcgat gaaattcgcc gggttacgcg cgccttcgcc agactgggca ccgaaaaagt gcgcctgaca 
0211 ggaggagagc cgtctttacg ccgcgacttt accgatatca tcgccgctgt gcgggaaaac gacgctatcc 
0281 gccagattgc ggtcacaacc aatggttacc gtctggaacg cgatgtggcg agctggcgcg atgcgggact 
0351 tactggcatt aacgtcagtg tcgacagtct ggacgcccgc cagtttcacg ctattaccgg gcaggataaa 
0421 ttcaaccagg tcatggcagg gattgatgct gcatttgagg ccggttttga gaaggtcaaa gtcaataccg 
0491 tgctgatgcg tgatgttaat catcaccagc tcgacacctt tctgaactgg atccagcatc gccctatcca 
0561 gctgcgtttc atcgaactga tggaaacggg cgagggcagc gagctcttcc gtaagcatca catctctggt 
0631 caggttctgc gtgacgagct actgcgtcgc ggctggatcc accaattacg tcaacgcagc gacggtcccg 
0701 cgcaagtctt ttgccatcca gattacgccg gagagattgg ccttatcatg ccgtatgaaa aagacttctg 
0771 cgccacttgc aaccgcctgc gcgtttcctc cattggtaaa ctccatctct gcctgtttgg tgaaggcggc 
0841 gttaacctgc gcgatctgct ggaagacgat acccagcaac aggcgctgga agcgcgtatt tcagcggcgc 
0911 tgcgggagaa gaaacagacc catttcctgc atcaaaacaa caccggtatt acgcaaaact tatcgtacat 
0981 tggcggctaa AACGTCAAAA GGAGAGATCA GATGAGTCAG GTAAGCACTG AATTTATCCC GACCCGTATT 
1051 GCTATTCTTA CGGTTTCTAA TCGTCGCGGT GAAGAAGACG