MG1655
W3110
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Gene Report : bioF
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Proc Natl Acad Sci U S A. 1990;87(1):360-3.
Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K-12.
Lejeune P, Danchin A. ( 2404280 )
     Deletion 15 (LD)  ,  DE(pgl-grxA)502  ,  DE(galETK-chlA)  ,  15+dps (LD)  ,  OCR48,49-5 (D)  ,  OCR48,50-8 (LD 15D::Cm) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  bioF
Related strains
     Strains Search Search strains by gene name:  bioF   Search strains by all related name:  bioF b0776 ECK0765 JW0759

General information  (Go to Linear View:)
 Gene Name bioF  
 Alternative name b0776,ECK0765,JW0759  
 Location, Length 809,604 - 810,758 (  +  ) ;   17.45 min ; 1155 (bp) ,   384 (aa) Go to Linear View
 Product 8-amino-7-oxononanoate synthase  
 Operon Name bioBFCD  
 Note GO_process: GO:0009102 - biotin biosynthetic process  
 Function enzyme; Biosynthesis of cofactors, carriers: Biotin  
 Gene Ontology GO:0003824 ; catalytic activity ( bioF )
GO:0008152 ; metabolic process ( bioF )
GO:0008710 ; 8-amino-7-oxononanoate synthase activity ( bioF )
GO:0009058 ; biosynthetic process ( bioF )
GO:0009102 ; biotin biosynthetic process ( bioF )
GO:0016740 ; transferase activity ( bioF )
GO:0016769 ; transferase activity, transferring nitrogenous groups ( bioF )
GO:0030170 ; pyridoxal phosphate binding ( bioF )
 PID 1786993  
 EC number
  (KEGG Pathway)   		2.3.1.47  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) BIOF_ECOLI ( P12998 )
    -  Protein name 8-amino-7-oxononanoate synthase  
    -  Synonyms EC 2.3.1.47, AONS, 8-amino-7- ketopelargonate synthase, 7-keto-8-amino-pelargonic acid synthetase, 7-KAP synthetase, L-alanine--pimelyl CoA ligase  
    -  Gene name Name=bioF; OrderedLocusNames=b0776;  

 Linear View (Whole Mode)
View Location
   800.0  –  825.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP bioF (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of 8-Amino-7-Oxonanoate Synthase (Or 7- Keto-8aminipelargonate Or Kapa Synthase) Complexed With Plp And The Product 8(S)-Amino-7-Oxonanonoate (Or Kapa). The Enzyme Of Biotin Biosynthetic Pathway.  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST 8-amino-7-oxononanoate synthase  
         PSI-BLAST Serine hydroxymethyltransferase
    nr     (database updated : 2007.02.20 )
         BLAST 8-amino-7-oxononanoate synthase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AA_TRANSFER_CLASS_2     

Other Cross-Reference
    COG COG0156H 
    EcoCyc bioF 

MMBR References
Proc Natl Acad Sci U S A. 1968;61(3):956-62.
A deletion analysis of prophage lambda and adjacent genetic regions.
Adhya S, Cleary P, Campbell A. ( 5246560 )
Mol Microbiol. 1996;19(3):645-7.
To be free or not: the fate of pimelate in Bacillus sphaericus and in Escherichia coli.
Lemoine Y, Wach A, Jeltsch JM. ( 8830257 )
J. Microbiol. Serol. 1969;35:505-521
A genetical and biochemical study of chlorate-resistant mutants of Salmonella typhimurium. Antonie Leeuwenhoek.
Stouthamer, A. H.
Gene. 1982;19(1):93-103.
A physical map of the Escherichia coli bio operon.
Szybalski EH, Szybalski W. ( 6292053 )

Sequences
Amino acid
FASTA format 
0001 MSWQEKINAA LDARRAADAL RRRYPVAQGA GRWLVADDRQ YLNFSSNDYL GLSHHPQIIR AWQQGAEQFG 
0071 IGSGGSGHVS GYSVVHQALE EELAEWLGYS RALLFISGFA ANQAVIAAMM AKEDRIAADR LSHASLLEAA 
0141 SLSPSQLRRF AHNDVTHLAR LLASPCPGQQ MVVTEGVFSM DGDSAPLAEI QQVTQQHNGW LMVDDAHGTG 
0211 VIGEQGRGSC WLQKVKPELL VVTFGKGFGV SGAAVLCSST VADYLLQFAR HLIYSTSMPP AQAQALRASL 
0281 AVIRSDEGDA RREKLAALIT RFRAGVQDLP FTLADSCSAI QPLIVGDNSR ALQLAEKLRQ QGCWVTAIRP 
0351 PTVPAGTARL RLTLTAAHEM QDIDRLLEVL HGNG
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             AGCAAACTGC CGTGCTGGCA GGGGATAACG 
-070 AACAACAGCA ACGTCTTGAA CAGGCGCTGA TGACCCCGGA CACCGACGAA TATTACAACG CGGCAGCATT 
0001 atgagctggc aggagaaaat caacgcggcg ctcgatgcgc ggcgtgctgc cgatgccctg cgtcgccgtt 
0071 atccggtggc gcaaggagcc ggacgctggc tggtggcgga tgatcgccag tatctgaact tttccagtaa 
0141 cgattattta ggtttaagcc atcatccgca aattatccgt gcctggcagc agggggcgga gcaatttggc 
0211 atcggtagcg gcggctccgg tcacgtcagc ggttatagcg tggtgcatca ggcactggaa gaagagctgg 
0281 ccgagtggct tggctattcg cgggcactgc tgtttatctc tggtttcgcc gctaatcagg cagttattgc 
0351 cgcgatgatg gcgaaagagg accgtattgc tgccgaccgg cttagccatg cctcattgct ggaagctgcc 
0421 agtttaagcc cgtcgcagct tcgccgtttt gctcataacg atgtcactca tttggcgcga ttgcttgctt 
0491 ccccctgtcc ggggcagcaa atggtggtga cagaaggcgt gttcagcatg gacggcgata gtgcgccact 
0561 ggcggaaatc cagcaggtaa cgcaacagca caatggctgg ttgatggtcg atgatgccca cggcacgggc 
0631 gttatcgggg agcaggggcg cggcagctgc tggctgcaaa aggtaaaacc agaattgctg gtagtgactt 
0701 ttggcaaagg atttggcgtc agcggggcag cggtgctttg ctccagtacg gtggcggatt atctgctgca 
0771 attcgcccgc caccttatct acagcaccag tatgccgccc gctcaggcgc aggcattacg tgcgtcgctg 
0841 gcggtcattc gcagtgatga gggtgatgca cggcgcgaaa aactggcggc actcattacg cgttttcgtg 
0911 ccggagtaca ggatttgccg tttacgcttg ctgattcatg cagcgccatc cagccattga ttgtcggtga 
0981 taacagccgt gcgttacaac tggcagaaaa actgcgtcag caaggctgct gggtcacggc gattcgcccg 
1051 ccaaccgtac ccgctggtac tgcgcgactg cgcttaacgc taaccgctgc gcatgaaatg caggatatcg 
1121 accgtctgct ggaggtgctg catggcaacg gttaaTAAAC AAGCCATTGC AGCGGCATTT GGTCGGGCAG 
1191 CCGCACACTA TGAGCAACAT GCAGATCTAC AGCGCCAGAG TGCTGACGCC TTACTGGCAA TGCTT