MG1655
W3110
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Gene Report : leuA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR30 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  leuA
Related strains
     Strains Search Search strains by gene name:  leuA   Search strains by all related name:  leuA b0074 ECK0076 JW0073

General information  (Go to Linear View:)
 Gene Name leuA  
 Alternative name b0074,ECK0076,JW0073  
 Location, Length 81,958 - 83,529 (  -  ) ;   1.77 min ; 1572 (bp) ,   523 (aa) Go to Linear View
 Product 2-isopropylmalate synthase  
 Operon Name leuLABCD  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009098 - leucine biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Leucine  
 Gene Ontology GO:0003824; catalytic activity ( leuA )
GO:0003852; 2-isopropylmalate synthase activity ( leuA )
GO:0008152; metabolic process ( leuA )
GO:0008652; amino acid biosynthetic process ( leuA )
GO:0009082; branched chain family amino acid biosynthetic process ( leuA )
GO:0009098; leucine biosynthetic process ( leuA )
GO:0016740; transferase activity ( leuA )
GO:0019752; carboxylic acid metabolic process ( leuA )
GO:0046912; transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer ( leuA )
 PID 1786261  
 EC number
  (KEGG Pathway)
 
2.3.3.13  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene leuA ( EG11226 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) LEU1_ECOLI ( P09151 ; P78044 )
    -  Protein name 2-isopropylmalate synthase  
    -  Synonyms EC 2.3.3.13, Alpha-isopropylmalate synthase, Alpha-IPM synthetase  
    -  Gene name Name=leuA; OrderedLocusNames=b0074;  

 Linear View (Whole Mode)
View Location
   70.0  –  95.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP leuA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Leua From Mycobacterium Tuberculosis  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST 2-isopropylmalate synthase  
         PSI-BLAST 2-isopropylmalate synthase (Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
    nr     (database updated : 2007.02.20 )
         BLAST 2-isopropylmalate synthase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    AIPM_HOMOCIT_SYNTH_1    AIPM_HOMOCIT_SYNTH_2     

Other Cross-Reference
    COG COG0119E 
    EcoCyc leuA 

MMBR References
J Mol Biol. 1983;170(1):39-59.
Transcription initiation sites of the leucine operons of Salmonella typhimurium and Escherichia coli.
Gemmill RM, Jones JW, Haughn GW, Calvo JM. ( 6195343 )
J Bacteriol. 1991;173(5):1634-41.
Transcription attenuation-mediated control of leu operon expression: influence of the number of Leu control codons.
Bartkus JM, Tyler B, Calvo JM. ( 1999384 )
J Mol Biol. 1981;149(4):579-97.
Control of leu operon expression in Escherichia coli by a transcription attenuation mechanism.
Wessler SR, Calvo JM. ( 6171647 )
J Bacteriol. 1977;129(2):1078-90.
Isolation and characterization of lambda pleu bacteriophages.
Davis MG, Calvo JM. ( 320178 )

Sequences
Amino acid
FASTA format
0001 MSQQVIIFDT TLRDGEQALQ ASLSVKEKLQ IALALERMGV DVMEVGFPVS SPGDFESVQT IARQVKNSRV 
0071 CALARCVEKD IDVAAESLKV AEAFRIHTFI ATSPMHIATK LRSTLDEVIE RAIYMVKRAR NYTDDVEFSC 
0141 EDAGRTPIAD LARVVEAAIN AGATTINIPD TVGYTMPFEF AGIISGLYER VPNIDKAIIS VHTHDDLGLA 
0211 VGNSLAAVHA GARQVEGAMN GIGERAGNCS LEEVIMAIKV RKDILNVHTA INHQEIWRTS QLVSQICNMP 
0281 IPANKAIVGS GAFAHSSGIH QDGVLKNREN YEIMTPESIG LNQIQLNLTS RSGRAAVKHR MDEMGYKESE 
0351 YNLDNLYDAF LKLADKKGQV FDYDLEALAF IGKQQEEPEH FRLDYFSVQS GSNDIATAAV KLACGEEVKA 
0421 EAANGNGPVD AVYQAINRIT EYNVELVKYS LTAKGHGKDA LGQVDIVANY NGRRFHGVGL ATDIVESSAK 
0491 AMVHVLNNIW RAAEVEKELQ RKAQHNENNK ETV

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             agcattaagc cagcacgcag tcaaacaaaa 
-070 aacccgcgcc attgcgcggg tttttttatg cccgaagcga ggcgctctaa aagagacaag gacccaaacc 
0001 atgagccagc aagtcattat tttcgatacc acattgcgcg acggtgaaca ggcgttacag gcaagcttga 
0071 gtgtgaaaga aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt gacgtgatgg aagtcggttt 
0141 ccccgtctct tcgccgggcg attttgaatc ggtgcaaacc atcgcccgcc aggttaaaaa cagccgcgta 
0211 tgtgcgttag ctcgctgcgt ggaaaaagat atcgacgtgg cggccgaatc cctgaaagtc gccgaagcct 
0281 tccgtattca tacctttatt gccacttcgc caatgcacat cgccaccaag ctgcgcagca cgctggacga 
0351 ggtgatcgaa cgcgctatct atatggtgaa acgcgcccgt aattacaccg atgatgttga attttcttgc 
0421 gaagatgccg ggcgtacacc cattgccgat ctggcgcgag tggtcgaagc ggcgattaat gccggtgcca 
0491 ccaccatcaa cattccggac accgtgggct acaccatgcc gtttgagttc gccggaatca tcagcggcct 
0561 gtatgaacgc gtgcctaaca tcgacaaagc cattatctcc gtacataccc acgacgattt gggcctggcg 
0631 gtcggaaact cactggcggc ggtacatgcc ggtgcacgcc aggtggaagg cgcaatgaac gggatcggcg 
0701 agcgtgccgg aaactgttcc ctggaagaag tcatcatggc gatcaaagtt cgtaaggata ttctcaacgt 
0771 ccacaccgcc attaatcacc aggagatatg gcgcaccagc cagttagtta gccagatttg taatatgccg 
0841 atcccggcaa acaaagccat tgttggcagc ggcgcattcg cacactcctc cggtatacac caggatggcg 
0911 tgctgaaaaa ccgcgaaaac tacgaaatca tgacaccaga atctattggt ctgaaccaaa tccagctgaa 
0981 tctgacctct cgttcggggc gtgcggcggt gaaacatcgc atggatgaga tggggtataa agaaagtgaa 
1051 tataatttag acaatttgta cgatgctttc ctgaagctgg cggacaaaaa aggtcaggtg tttgattacg 
1121 atctggaggc gctggccttc atcggtaagc agcaagaaga gccggagcat ttccgtctgg attacttcag 
1191 cgtgcagtct ggctctaacg atatcgccac cgccgccgtc aaactggcct gtggcgaaga agtcaaagca 
1261 gaagccgcca acggtaacgg tccggtcgat gccgtctatc aggcaattaa ccgcatcact gaatataacg 
1331 tcgaactggt gaaatacagc ctgaccgcca aaggccacgg taaagatgcg ctgggtcagg tggatatcgt 
1401 cgctaactac aacggtcgcc gcttccacgg cgtcggcctg gctaccgata ttgtcgagtc atctgccaaa 
1471 gccatggtgc acgttctgaa caatatctgg cgtgccgcag aagtcgaaaa agagttgcaa cgcaaagctc 
1541 aacacaacga aaacaacaag gaaaccgtgt gatgtcgaag aattaccata ttgccgtatt gccgggggac 
1611 ggtattggtc cggaagtgat gacccaggcg ctgaaagtgc tggatgccgt gcgcaaccgc tt