MG1655
W3110
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Gene Report : leuC
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR30 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  leuC
Related strains
     Strains Search Search strains by gene name:  leuC   Search strains by all related name:  leuC b0072 ECK0074 JW0071

General information  (Go to Linear View:)
 Gene Name leuC  
 Alternative name b0072,ECK0074,JW0071  
 Location, Length 79,464 - 80,864 (  -  ) ;   1.71 min ; 1401 (bp) ,   466 (aa) Go to Linear View
 Product 3-isopropylmalate dehydratase large subunit  
 Operon Name leuLABCD  
 Note 3-isopropylmalate isomerase (dehydratase) subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009098 - leucine biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Leucine  
 Gene Ontology GO:0003861 ; 3-isopropylmalate dehydratase activity ( leuC )
GO:0005506 ; iron ion binding ( leuC )
GO:0008152 ; metabolic process ( leuC )
GO:0008652 ; amino acid biosynthetic process ( leuC )
GO:0009082 ; branched chain family amino acid biosynthetic process ( leuC )
GO:0009098 ; leucine biosynthetic process ( leuC )
GO:0016829 ; lyase activity ( leuC )
GO:0016836 ; hydro-lyase activity ( leuC )
GO:0046872 ; metal ion binding ( leuC )
GO:0051536 ; iron-sulfur cluster binding ( leuC )
GO:0051539 ; 4 iron, 4 sulfur cluster binding ( leuC )
 PID 1786259  
 EC number
  (KEGG Pathway)   		4.2.1.33  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene leuC ( EG11576 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) LEU2_ECOLI ( P30127 ; P78042; Q8FL77 )
    -  Protein name 3-isopropylmalate dehydratase large subunit  
    -  Synonyms EC 4.2.1.33, Isopropylmalate isomerase, Alpha-IPM isomerase, IPMI  
    -  Gene name Name=leuC; OrderedLocusNames=b0072, c0089;  

 Linear View (Whole Mode)
View Location
   70.0  –  95.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP leuC (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain , Aconitase (E.C.4.2.1.3) (Inactive (3fe-4s) Cluster Form)  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST 3-isopropylmalate dehydratase large subunit (Isopropylmalate isomerase) (Alpha-IPM isomerase) (IPMI)  
         PSI-BLAST 3-isopropylmalate dehydratase large subunit
    nr     (database updated : 2007.02.20 )
         BLAST isopropylmalate isomerase large subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    ATP_GTP_A    ACONITASE_1    ACONITASE_2     

Other Cross-Reference
    EcoCyc leuC 

MMBR References
J Bacteriol. 1977;129(2):1078-90.
Isolation and characterization of lambda pleu bacteriophages.
Davis MG, Calvo JM. ( 320178 )

Sequences
Amino acid
FASTA format 
0001 MAKTLYEKLF DAHVVYEAEN ETPLLYIDRH LVHEVTSPQA FDGLRAHGRP VRQPGKTFAT MDHNVSTQTK 
0071 DINACGEMAR IQMQELIKNC KEFGVELYDL NHPYQGIVHV MGPEQGVTLP GMTIVCGDSH TATHGAFGAL 
0141 AFGIGTSEVE HVLATQTLKQ GRAKTMKIEV QGKAAPGITA KDIVLAIIGK TGSAGGTGHV VEFCGEAIRD 
0211 LSMEGRMTLC NMAIEMGAKA GLVAPDETTF NYVKGRLHAP KGKDFDDAVA YWKTLQTDEG ATFDTVVTLQ 
0281 AEEISPQVTW GTNPGQVISV NDNIPDPASF ADPVERASAE KALAYMGLKP GIPLTEVAID KVFIGSCTNS 
0351 RIEDLRAAAE IAKGRKVAPG VQALVVPGSG PVKAQAEAEG LDKIFIEAGF EWRLPGCSMC LAMNNDRLNP 
0421 GERCASTSNR NFEGRQGRGG RTHLVSPAMA AAAAVTGHFA DIRNIK
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             aaggcattcg caccggggat ttagcccgtg 
-070 gcgctgccgc cgttagtacc gatgaaatgg gcgatatcat tgcccgctat gtagcagaag gggtgtaatc 
0001 atggctaaga cgttatacga aaaattgttc gacgctcacg ttgtgtacga agccgaaaac gaaaccccac 
0071 tgttatatat cgaccgccac ctggtgcatg aagtgacctc accgcaggcg ttcgatggtc tgcgcgccca 
0141 cggtcgcccg gtacgtcagc cgggcaaaac cttcgctacc atggatcaca acgtctctac ccagaccaaa 
0211 gacattaatg cctgcggtga aatggcgcgt atccagatgc aggaactgat caaaaactgc aaagaatttg 
0281 gcgtcgaact gtatgacctg aatcacccgt atcaggggat cgtccacgta atggggccgg aacagggcgt 
0351 caccttgccg gggatgacca ttgtctgcgg cgactcgcat accgccaccc acggcgcgtt tggcgcactg 
0421 gcctttggta tcggcacttc cgaagttgaa cacgtactgg caacgcaaac cctgaaacag ggccgcgcaa 
0491 aaaccatgaa aattgaagtc cagggcaaag ccgcgccggg cattaccgca aaagatatcg tgctggcaat 
0561 tatcggtaaa accggtagcg caggcggcac cgggcatgtg gtggagtttt gcggcgaagc aatccgtgat 
0631 ttaagcatgg aaggtcgtat gaccctgtgc aatatggcaa tcgaaatggg cgcaaaagcc ggtctggttg 
0701 caccggacga aaccaccttt aactatgtca aaggccgtct gcatgcgccg aaaggcaaag atttcgacga 
0771 cgccgttgcc tactggaaaa ccctgcaaac cgacgaaggc gcaactttcg ataccgttgt cactctgcaa 
0841 gcagaagaaa tttcaccgca ggtcacctgg ggcaccaatc ccggccaggt gatttccgtg aacgacaata 
0911 ttcccgatcc ggcttcgttt gccgatccgg ttgaacgcgc gtcggcagaa aaagcgctgg cctatatggg 
0981 gctgaaaccg ggtattccgc tgaccgaagt ggctatcgac aaagtgttta tcggttcctg taccaactcg 
1051 cgcattgaag atttacgcgc ggcagcggag atcgccaaag ggcgaaaagt cgcgccaggc gtgcaggcac 
1121 tggtggttcc cggctctggc ccggtaaaag cccaggcgga agcggaaggt ctggataaaa tctttattga 
1191 agccggtttt gaatggcgct tgcctggctg ctcaatgtgt ctggcgatga acaacgaccg tctgaatccg 
1261 ggcgaacgtt gtgcctccac cagcaaccgt aactttgaag gccgccaggg gcgcggcggg cgcacgcatc 
1331 tggtcagccc ggcaatggct gccgctgctg ctgtgaccgg acatttcgcc gacattcgca acattaaata 
1401 aggagcacac catggcagag aaatttatca aacacacagg cctggtggtt ccgctggatg ccgccaatgt 
1471 cgataccgat gcaatcatcc cgaaacagtt t