MG1655
W3110
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Gene Report : leuD
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCR30 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  leuD
Related strains
     Strains Search Search strains by gene name:  leuD   Search strains by all related name:  leuD b0071 ECK0073 JW0070

General information  (Go to Linear View:)
 Gene Name leuD  
 Alternative name b0071,ECK0073,JW0070  
 Location, Length 78,848 - 79,453 (  -  ) ;   1.70 min ; 606 (bp) ,   201 (aa) Go to Linear View
 Product 3-isopropylmalate dehydratase small subunit  
 Operon Name leuLABCD  
 Note isopropylmalate isomerase subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009098 - leucine biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Leucine  
 Gene Ontology GO:0003861; 3-isopropylmalate dehydratase activity ( leuD )
GO:0008152; metabolic process ( leuD )
GO:0008652; amino acid biosynthetic process ( leuD )
GO:0009082; branched chain family amino acid biosynthetic process ( leuD )
GO:0009098; leucine biosynthetic process ( leuD )
GO:0009316; 3-isopropylmalate dehydratase complex ( leuD )
GO:0016829; lyase activity ( leuD )
 PID 1786258  
 EC number
  (KEGG Pathway)
 
4.2.1.33  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene leuD ( EG11575 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) LEUD_ECOLI ( P30126 )
    -  Protein name 3-isopropylmalate dehydratase small subunit  
    -  Synonyms EC 4.2.1.33, Isopropylmalate isomerase, Alpha-IPM isomerase, IPMI  
    -  Gene name Name=leuD; OrderedLocusNames=b0071;  

 Linear View (Whole Mode)
View Location
   70.0  –  95.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP leuD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Smu.1381  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST 3-isopropylmalate dehydratase small subunit (Isopropylmalate isomerase) (Alpha-IPM isomerase) (IPMI)  
         PSI-BLAST 3-isopropylmalate dehydratase small subunit 1
    nr     (database updated : 2007.02.20 )
         BLAST isopropylmalate isomerase small subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0066E 
    EcoCyc leuD 

MMBR References
J Bacteriol. 1977;129(2):1078-90.
Isolation and characterization of lambda pleu bacteriophages.
Davis MG, Calvo JM. ( 320178 )

Sequences
Amino acid
FASTA format
0001 MAEKFIKHTG LVVPLDAANV DTDAIIPKQF LQKVTRTGFG AHLFNDWRFL DEKGQQPNPD FVLNFPQYQG 
0071 ASILLARENF GCGSSREHAP WALTDYGFKV VIAPSFADIF YGNSFNNQLL PVKLSDAEVD ELFALVKANP 
0141 GIHFDVDLEA QEVKAGEKTY RFTIDAFRRH CMMNGLDSIG LTLQHDDAIA AYEAKQPAFM N

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             cgcggcgggc gcacgcatct ggtcagcccg 
-070 gcaatggctg ccgctgctgc tgtgaccgga catttcgccg acattcgcaa cattaaataa ggagcacacc 
0001 atggcagaga aatttatcaa acacacaggc ctggtggttc cgctggatgc cgccaatgtc gataccgatg 
0071 caatcatccc gaaacagttt ttgcagaaag tgacccgtac gggttttggc gcgcatctgt ttaacgactg 
0141 gcgttttctg gatgaaaaag gccaacagcc aaacccggac ttcgtgctga acttcccgca gtatcagggc 
0211 gcttccattt tgctggcacg agaaaacttc ggctgtggct cttcgcgtga gcacgcgccc tgggcattga 
0281 ccgactacgg ttttaaagtg gtgattgcgc cgagttttgc tgacatcttc tacggcaata gctttaacaa 
0351 ccagctgctg ccggtgaaat taagcgatgc agaagtggac gaactgtttg cgctggtgaa agctaatccg 
0421 gggatccatt tcgacgtgga tctggaagcg caagaggtga aagcgggaga gaaaacctat cgctttacca 
0491 tcgatgcctt ccgccgccac tgcatgatga acggtctgga cagtattggg cttaccttgc agcacgacga 
0561 cgccattgcc gcttatgaag caaaacaacc tgcgtttatg aattaatccc cttgcccggt caaatgaccg 
0631 ggctttccgc tatcgtccac gtcatcaaac gtctttaacc tttgcggtta aaaataatgc gacaacagaa 
0701 ataacc