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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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DE(pgl-grxA)502
,
OCR48,49-18
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
sucB
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Related strains
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Strains Search
|
Search strains by gene name:
sucB
Search strains by all related name:
sucB b0727 ECK0715 JW0716
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General information (Go to Linear View: )
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Gene Name
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sucB
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Alternative name
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b0727,ECK0715,JW0716
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Location, Length
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760,745 - 761,962
(
+
)
;
16.40
min
;
1218
(bp) ,
405
(aa)
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Product
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dihydrolipoyltranssuccinase
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Operon Name
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sdhCDAB-sucABCD
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Note
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2-oxoglutarate dehydrogenase (dihydrolipoyltranssuccinase E2 component); GO_process: GO:0006099 - tricarboxylic acid cycle
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Function
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enzyme; Energy metabolism, carbon: TCA cycle
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Gene Ontology
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GO:0004149
;
dihydrolipoyllysine-residue succinyltransferase activity ( sucB )
GO:0005515
;
protein binding ( sucB )
GO:0006099
;
tricarboxylic acid cycle ( sucB )
GO:0008152
;
metabolic process ( sucB )
GO:0008415
;
acyltransferase activity ( sucB )
GO:0016740
;
transferase activity ( sucB )
GO:0031405
;
lipoic acid binding ( sucB )
GO:0045252
;
oxoglutarate dehydrogenase complex ( sucB )
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PID
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1786946
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EC number
(KEGG Pathway)
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2.3.1.61
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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sucB
(
EG10980
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
|
ODO2_ECOLI
(
P07016
)
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- Protein name
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Dihydrolipoyllysine-residue succinyltransferase component of 2- oxoglutarate dehydrogenase complex
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- Synonyms
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EC 2.3.1.61, E2, Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex
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- Gene name
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Name=sucB; OrderedLocusNames=b0727, z0881, ECs0752;
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MMBR References
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J Bacteriol. 1982;151(2):542-52.
Molecular cloning of four tricarboxylic acid cyclic genes of Escherichia coli.
Spencer ME, Guest JR.
(
6284701
)
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Mol Gen Genet. 1985;200(1):145-54.
Transcription analysis of the sucAB, aceEF and lpd genes of Escherichia coli.
Spencer ME, Guest JR.
(
3897791
)
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Eur J Biochem. 1984;141:361-374
Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase.
Spencer, M. E., M. G. Darlison, P. E. Stephens, and J. R. Guest.
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J Gen Microbiol. 1986;132(6):1753-62.
Cloning and expression of the succinyl-CoA synthetase genes of Escherichia coli K12.
Buck D, Spencer ME, Guest JR.
(
3543212
)
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| Sequences |
Amino acid
FASTA format
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0001 MSSVDILVPD LPESVADATV ATWHKKPGDA VVRDEVLVEI ETDKVVLEVP ASADGILDAV LEDEGTTVTS
0071 RQILGRLREG NSAGKETSAK SEEKASTPAQ RQQASLEEQN NDALSPAIRR LLAEHNLDAS AIKGTGVGGR
0141 LTREDVEKHL AKAPAKESAP AAAAPAAQPA LAARSEKRVP MTRLRKRVAE RLLEAKNSTA MLTTFNEVNM
0211 KPIMDLRKQY GEAFEKRHGI RLGFMSFYVK AVVEALKRYP EVNASIDGDD VVYHNYFDVS MAVSTPRGLV
0281 TPVLRDVDTL GMADIEKKIK ELAVKGRDGK LTVEDLTGGN FTITNGGVFG SLMSTPIINP PQSAILGMHA
0351 IKDRPMAVNG QVEILPMMYL ALSYDHRLID GRESVGFLVT IKELLEDPTR LLLDV
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 CCTCCGCCTC TCCGGCGGTA GGGTATATGT
-070 CCGTTCACCA GAAACAGCAA CAAGATCTGG TTAATGACGC GCTGAACGTC GAATAAATAA AGGATACACA
0001 atgagtagcg tagatattct ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc gcaacctggc
0071 ataaaaaacc cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc gaaactgaca aagtggtact
0141 ggaagtaccg gcatcagcag acggcattct ggatgcggtt ctggaagatg aaggtacaac ggtaacgtct
0211 cgtcagatcc ttggtcgcct gcgtgaaggc aacagcgccg gtaaagaaac cagcgccaaa tctgaagaga
0281 aagcgtccac tccggcgcaa cgccagcagg cgtctctgga agagcaaaac aacgatgcgt taagcccggc
0351 gatccgtcgc ctgctggctg aacacaatct cgacgccagc gccattaaag gcaccggtgt gggtggtcgt
0421 ctgactcgtg aagatgtgga aaaacatctg gcgaaagccc cggcgaaaga gtctgctccg gcagcggctg
0491 ctccggcggc gcaaccggct ctggctgcac gtagtgaaaa acgtgtcccg atgactcgcc tgcgtaagcg
0561 tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc atgctgacca cgttcaacga agtcaacatg
0631 aagccgatta tggatctgcg taagcagtac ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct
0701 ttatgtcctt ctacgtgaaa gcggtggttg aagccctgaa acgttacccg gaagtgaacg cttctatcga
0771 cggcgatgac gtggtttacc acaactattt cgacgtcagc atggcggttt ctacgccgcg cggcctggtg
0841 acgccggttc tgcgtgatgt cgataccctc ggcatggcag acatcgagaa gaaaatcaaa gagctggcag
0911 tcaaaggccg tgacggcaag ctgaccgttg aagatctgac cggtggtaac ttcaccatca ccaacggtgg
0981 tgtgttcggt tccctgatgt ctacgccgat catcaacccg ccgcagagcg caattctggg tatgcacgct
1051 atcaaagatc gtccgatggc ggtgaatggt caggttgaga tcctgccgat gatgtacctg gcgctgtcct
1121 acgatcaccg tctgatcgat ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt tgctggaaga
1191 tccgacgcgt ctgctgctgg acgtgtagTA GTTTAAGTTT CACCTGCACT GTAGACCGGA TAAGGCATTA
1261 TCGCCTTCTC CGGCAATTGA AGCCTGATGC GACGCTGACG CGTCTTATCA GGCCTACG
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