MG1655
W3110
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Gene Report : sdhB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(pgl-grxA)502  ,  OCR48,49-12 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  sdhB
Related strains
     Strains Search Search strains by gene name:  sdhB   Search strains by all related name:  sdhB b0724 ECK0713 JW0714

General information  (Go to Linear View:)
 Gene Name sdhB  
 Alternative name b0724,ECK0713,JW0714  
 Location, Length 756,912 - 757,628 (  +  ) ;   16.31 min ; 717 (bp) ,   238 (aa) Go to Linear View
 Product succinate dehydrogenase, FeS subunit  
 Operon Name sdhCDAB-sucABCD  
 Note succinate dehydrogenase, iron sulfur protein; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006099 - tricarboxylic acid cycle; GO_process: GO:0009060 - aerobic respiration  
 Function enzyme; Energy metabolism, carbon: TCA cycle  
 Gene Ontology GO:0000104 ; succinate dehydrogenase activity ( sdhB )
GO:0005506 ; iron ion binding ( sdhB )
GO:0005515 ; protein binding ( sdhB )
GO:0005886 ; plasma membrane ( sdhB )
GO:0006099 ; tricarboxylic acid cycle ( sdhB )
GO:0006118 ; electron transport ( sdhB )
GO:0006810 ; transport ( sdhB )
GO:0009055 ; electron carrier activity ( sdhB )
GO:0016020 ; membrane ( sdhB )
GO:0016491 ; oxidoreductase activity ( sdhB )
GO:0046872 ; metal ion binding ( sdhB )
GO:0051536 ; iron-sulfur cluster binding ( sdhB )
GO:0051537 ; 2 iron, 2 sulfur cluster binding ( sdhB )
GO:0051538 ; 3 iron, 4 sulfur cluster binding ( sdhB )
GO:0051539 ; 4 iron, 4 sulfur cluster binding ( sdhB )
 PID 1786943  
 EC number
  (KEGG Pathway)
 
1.3.99.1  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene sdhB ( EG10932 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) DHSB_ECOLI ( P07014 )
    -  Protein name Succinate dehydrogenase iron-sulfur protein  
    -  Synonyms EC 1.3.99.1  
    -  Gene name Name=sdhB; OrderedLocusNames=b0724;  

 Linear View (Whole Mode)
View Location
   745.0  –  770.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP sdhB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain B, Complex Ii (Succinate Dehydrogenase) From E. Coli With Ubiquinone Bound  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Succinate dehydrogenase iron-sulfur protein  
         PSI-BLAST Succinate dehydrogenase [ubiquinone] iron-sulfur protein, mitochondrial precursor
    nr     (database updated : 2007.02.20 )
         BLAST succinate dehydrogenase, FeS subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    4FE4S_FERREDOXIN     

Other Cross-Reference
    COG COG0479C 
    EcoCyc sdhB 

MMBR References
Mol Microbiol. 1995;15(3):473-82.
Regulation of succinate dehydrogenase (sdhCDAB) operon expression in Escherichia coli in response to carbon supply and anaerobiosis: role of ArcA and Fnr.
Park SJ, Tseng CP, Gunsalus RP. ( 7783618 )
J Gen Microbiol. 1986;132(12):3239-51.
Transcript analysis of the citrate synthase and succinate dehydrogenase genes of Escherichia coli K12.
Wilde RJ, Guest JR. ( 3309132 )
Biochem J. 1984;222(2):519-34.
Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli.
Wood D, Darlison MG, Wilde RJ, Guest JR. ( 6383359 )
J Bacteriol. 1991;173(5):1569-70.
E. coli map. Physical map locations of genes encoding components of the aerobic respiratory chain of Escherichia coli.
Calhoun MW, Newton G, Gennis RB. ( 1847904 )
Biochem J. 1984;223(2):507-17.
Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli.
Darlison MG, Guest JR. ( 6388571 )

Sequences
Amino acid
FASTA format
0001 MRLEFSIYRY NPDVDDAPRM QDYTLEADEG RDMMLLDALI QLKEKDPSLS FRRSCREGVC GSDGLNMNGK 
0071 NGLACITPIS ALNQPGKKIV IRPLPGLPVI RDLVVDMGQF YAQYEKIKPY LLNNGQNPPA REHLQMPEQR 
0141 EKLDGLYECI LCACCSTSCP SFWWNPDKFI GPAGLLAAYR FLIDSRDTET DSRLDGLSDA FSVFRCHSIM 
0211 NCVSVCPKGL NPTRAIGHIK SMLLQRNA

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             AGAGTCGGAA TCCATGACGC GCCGAAGCGT 
-070 CAACATGGAA CCGAAACTGC GCCCGGCATT CCCGCCGAAG ATTCGTACTT ACTAATGCGG AGACAGGAAA 
0001 atgagactcg agttttcaat ttatcgctat aacccggatg ttgatgatgc tccgcgtatg caggattaca 
0071 ccctggaagc ggatgaaggt cgcgacatga tgctgctgga tgcgcttatc cagctaaaag agaaagatcc 
0141 cagcctgtcg ttccgccgct cctgccgtga aggtgtgtgc ggttccgacg gtctgaacat gaacggcaag 
0211 aatggtctgg cctgtattac cccgatttcg gcactcaacc agccgggcaa gaagattgtg attcgcccgc 
0281 tgccaggttt accggtgatc cgcgatttgg tggtagacat gggacaattc tatgcgcaat atgagaaaat 
0351 taagccttac ctgttgaata atggacaaaa tccgccagct cgcgagcatt tacagatgcc agagcagcgc 
0421 gaaaaactcg acgggctgta tgaatgtatt ctctgcgcat gttgttcaac ctcttgtccg tctttctggt 
0491 ggaatcccga taagtttatc ggcccggcag gcttgttagc ggcatatcgt ttcctgattg atagccgtga 
0561 taccgagact gacagccgcc tcgacggttt gagtgatgca ttcagcgtat tccgctgtca cagcatcatg 
0631 aactgcgtca gtgtatgtcc gaaggggctg aacccgacgc gcgccatcgg ccatatcaag tcgatgttgt 
0701 tgcaacgtaa tgcgtaaACC GTAGGCCTGA TAAGACGCGC AAGCGTCGCA TCAGGCAACC AGTGCCGGAT 
0771 GCGGCGTGAA CGCCTTATCC GGCCTACAAG TCATTACCCG TAGGCCT