MG1655
W3110
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Gene Report : thiB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(araA-leu)7697  ,  OCR30 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  thiB
Related strains
     Strains Search Search strains by gene name:  thiB   Search strains by all related name:  thiB b0068 ECK0069 f327 JW0067 sfuA tbpA yabL

General information  (Go to Linear View:)
 Gene Name thiB  
 Alternative name b0068,ECK0069,f327,JW0067,sfuA,tbpA,yabL  
 Location, Length 74,497 - 75,480 (  -  ) ;   1.61 min ; 984 (bp) ,   327 (aa) Go to Linear View
 Product thiamin transporter subunit  
 Operon Name sgrR-sroA-tbpA-thiPQ  
 Note thiamin-binding periplasmic protein; periplasmic-binding component of ABC superfamily; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0009228 - thiamin biosynthetic process  
 Function putative transport; Transport of small molecules: Other  
 Gene Ontology GO:0005215 ; transporter activity ( thiB )
GO:0005524 ; ATP binding ( thiB )
GO:0006810 ; transport ( thiB )
GO:0030288 ; outer membrane-bounded periplasmic space ( thiB )
GO:0042597 ; periplasmic space ( thiB )
GO:0042626 ; ATPase activity, coupled to transmembrane movement of substances ( thiB )
 PID 1786255  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene tbpA ( EG11574 )  
    Number of removed 18 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) TBPA_ECOLI ( P31550 ; P75637 )
    -  Protein name Thiamine-binding periplasmic protein precursor.  
    -  Synonyms  
    -  Gene name Name=tbpA; Synonyms=thiB; OrderedLocusNames=b0068;  

 Linear View (Whole Mode)
View Location
   65.0  –  90.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP tbpA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Ferric Binding Protein From Campylobacter Jejuni  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Thiamine-binding periplasmic protein precursor  
         PSI-BLAST Thiamine-binding periplasmic protein precursor
    nr     (database updated : 2007.02.20 )
         BLAST thiamin binding protein [Escherichia coli]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    SBP_BACTERIAL_1     

Other Cross-Reference
    COG COG4143H 
    EcoCyc thiB 

MMBR References
GenBank submission U09984. 1994
E. coli periplasmic thiamin binding protein: cloning, overexpression, purification, and characterization.
Hollenbach, A. D., K. A. Dickson, and M. W. Washabaugh.
J Bacteriol. 1993;175:982-992
Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli.
Vander Horn, P. B., A. D. Backstrom, V. J. Stewart, and T. P. Begley.

Sequences
Amino acid
FASTA format
0001 MLKKCLPLLL LCTAPVFAKP VLTVYTYDSF AADWGPGPVV KKAFEADCNC ELKLVALEDG VSLLNRLRME 
0071 GKNSKADVVL GLDNNLLDAA SKTGLFAKSG VAADAVNVPG GWNNDTFVPF DYGYFAFVYD KNKLKNPPQS 
0141 LKELVESDQN WRVIYQDPRT STPGLGLLLW MQKVYGDDAP QAWQKLAKKT VTVTKGWSEA YGLFLKGESD 
0211 LVLSYTTSPA YHILEEKKDN YAAANFSEGH YLQVEVAART AASKQPELAQ KFLQFMVSPA FQNAIPTGNW 
0281 MYPVANVTLP AGFEKLTKPA TTLEFTPAEV AAQRQAWISE WQRAVSR

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             tgcgctgaga aaatacccgt cgaacctgat 
-070 ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc actctttttt gaggtgcaaa 
0001 gtgttaaaaa aatgtctgcc cctgctgttg ctgtgcacag cgcccgtttt cgctaaaccc gttctgactg 
0071 tttataccta cgattccttc gccgccgact gggggcctgg tccggtggtt aaaaaagcct ttgaagccga 
0141 ctgtaattgc gaactgaaac tggtggcgct ggaagatggc gtttcgcttc tcaaccgtct acggatggaa 
0211 ggcaaaaaca gtaaagccga tgtggtgctg gggctggata acaacctgtt agacgccgcc agtaaaaccg 
0281 gactgtttgc caaaagcggt gtggcagcgg atgccgttaa cgttcccggc ggctggaata atgacacttt 
0351 cgtaccgttt gattatggct acttcgcctt cgtttatgac aagaacaaac tgaaaaaccc gccacaaagc 
0421 ctgaaagaac tggttgagag cgatcaaaac tggcgggtga tttatcagga tccgcgcacc agtacaccgg 
0491 ggctgggtct gttgctatgg atgcaaaaag tctatggcga tgacgcccca caagcctggc agaaactggc 
0561 gaagaaaacg gtcacggtca ccaaaggctg gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat 
0631 ctggtactga gttacaccac ctctccggct tatcacattc tcgaagagaa gaaagataac tacgccgccg 
0701 cgaacttcag cgaaggtcac tatctgcaag tggaagtcgc cgcccgcacc gctgccagca agcagccgga 
0771 gctggcgcaa aaattcctcc agtttatggt ttctccggct ttccagaatg cgatcccaac cggcaactgg 
0841 atgtatccgg tggcaaacgt cacgctgcct gccggttttg aaaaattgac caaacccgca accacgttgg 
0911 agttcacgcc agccgaagtg gcggcacaac gtcaggcatg gattagcgaa tggcaacgcg ccgtcagccg 
0981 ttaattcccg gctggttaat tccaggtgta agcgccacca cgctggtggt agcggttgcg ctggcggcgt 
1051 ttctcgccct gtggtggaac gcgccgcagg atga