|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Cell. 1994;77(3):413-26.
SeqA: a negative modulator of replication initiation in E. coli.
Lu M, Campbell JL, Boye E, Kleckner N.
(
8011018
)
|
|
|
Deletion
|
OCR48
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
seqA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
seqA
Search strains by all related name:
seqA b0687 ECK0675 hsm-1 JW0674
|
General information (Go to Linear View: )
|
|
Gene Name
|
seqA
|
|
Alternative name
|
b0687,ECK0675,hsm-1,JW0674
|
|
Location, Length
|
712,210 - 712,755
(
+
)
;
15.35
min
;
546
(bp) ,
181
(aa)
|
|
Product
|
regulatory protein for replication initiation
|
|
Operon Name
|
seqA-pgm
|
|
Note
|
negative modulator of initiation of replication; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006261 - DNA-dependent DNA replication
|
|
Function
|
regulator; DNA - replication, repair,restriction/modification
|
|
Gene Ontology
|
GO:0003677
;
DNA binding ( seqA )
GO:0005515
;
protein binding ( seqA )
GO:0008156
;
negative regulation of DNA replication ( seqA )
GO:0032297
;
negative regulation of DNA replication initiation ( seqA )
|
|
PID
|
1786903
|
EC number
(KEGG Pathway)
|
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
seqA
(
EG12197
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
SEQA_ECOLI
(
P36658
)
|
|
- Protein name
|
SeqA protein.
|
|
- Synonyms
|
|
|
- Gene name
|
Name=seqA; OrderedLocusNames=b0687, c0774, SF0606, S0617;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
seqA
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of E. Coli Seqa Complexed With Hemimethylated Dna
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Protein seqA
|
|
PSI-BLAST
|
Protein seqA
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
SeqA protein [Escherichia coli CFT073]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
AMIDATION
|
|
MMBR References
|
Curr Biol. 1994;4(10):945-6.
Replication initiation. A new controller in Escherichia coli.
Baker TA.
(
7850437
)
|
Cell. 1994;77(3):413-26.
SeqA: a negative modulator of replication initiation in E. coli.
Lu M, Campbell JL, Boye E, Kleckner N.
(
8011018
)
|
Cell. 1995;82(6):927-36.
E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration.
Slater S, Wold S, Lu M, Boye E, Skarstad K, Kleckner N.
(
7553853
)
|
Mol Microbiol. 1994;14(4):763-72.
SeqA limits DnaA activity in replication from oriC in Escherichia coli.
von Freiesleben U, Rasmussen KV, Schaechter M.
(
7891562
)
|
Proc Natl Acad Sci U S A. 1996;93:12206-12211
Coordinating DNA replication initiation with cell growth: differential roles for DnaA and SeqA proteins.
Boye, E., T. Stokke, N. Kleckner, and K. Skarstad.
|
| Sequences |
Amino acid
FASTA format
|
0001 MKTIEVDDEL YSYIASHTKH IGESASDILR RMLKFSAASQ PAAPVTKEVR VASPAIVEAK PVKTIKDKVR
0071 AMRELLLSDE YAEQKRAVNR FMLLLSTLYS LDAQAFAEAT ESLHGRTRVY FAADEQTLLK NGNQTKPKHV
0141 PGTPYWVITN TNTGRKCSMI EHIMQSMQFP AELIEKVCGT I
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CGAGTTTTTC TGCAAGCCAG GCTTGACGCT
-070 ATCCGCTGCC GGGATTTATT CATATACTCC TGGCGACTTG TATTCAGCTA AGACACTGCA CTGGATTAAG
0001 atgaaaacga ttgaagttga tgatgaactc tacagctata ttgccagcca cactaagcat atcggcgaga
0071 gcgcatccga cattttacgg cgtatgttga aattttccgc cgcatcacag cctgctgctc cggtgacgaa
0141 agaggttcgc gttgcgtcac ctgctatcgt cgaagcgaag ccggtcaaaa cgattaaaga caaggttcgc
0211 gcaatgcgtg aacttctgct ttcggatgaa tacgcagagc aaaagcgagc ggtcaatcgc tttatgctgc
0281 tgttgtctac actatattct cttgacgccc aggcgtttgc cgaagcaacg gaatcgttgc acggtcgtac
0351 acgcgtttac tttgcggcag atgaacaaac gctgctgaaa aatggtaatc agaccaagcc gaaacatgtg
0421 ccaggcacgc cgtattgggt gatcaccaac accaacaccg gccgtaaatg cagcatgatc gaacacatca
0491 tgcagtcgat gcaattcccg gcggaattga ttgagaaggt ttgcggaact atctaaAACG TTGCAGACAA
0561 AGGACAAAGC AATGGCAATC CACAATCGTG CAGGCCAACC TGCACAACAG AGTGATTTGA TTAACGTCGC
0631 CCAACTGACG GCGCAA
|
