MG1655
W3110
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Gene Report : nagA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR47,48-1 (D)  ,  OCR47-3 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  nagA
Related strains
     Strains Search Search strains by gene name:  nagA   Search strains by all related name:  nagA b0677 ECK0665 JW0663

General information  (Go to Linear View:)
 Gene Name nagA  
 Alternative name b0677,ECK0665,JW0663  
 Location, Length 700,826 - 701,974 (  -  ) ;   15.11 min ; 1149 (bp) ,   382 (aa) Go to Linear View
 Product N-acetylglucosamine-6-phosphate deacetylase  
 Operon Name nagBACD  
 Note GO_process: GO:0046349 - amino sugar biosynthetic process  
 Function enzyme; Central intermediary metabolism: Amino sugars  
 Gene Ontology GO:0005975 ; carbohydrate metabolic process ( nagA )
GO:0006044 ; N-acetylglucosamine metabolic process ( nagA )
GO:0008448 ; N-acetylglucosamine-6-phosphate deacetylase activity ( nagA )
GO:0016787 ; hydrolase activity ( nagA )
GO:0016810 ; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds ( nagA )
 PID 1786892  
 EC number
  (KEGG Pathway)
 
3.5.1.25  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) NAGA_ECOLI ( P15300 )
    -  Protein name N-acetylglucosamine-6-phosphate deacetylase  
    -  Synonyms EC 3.5.1.25, GlcNAc 6-P deacetylase  
    -  Gene name Name=nagA; OrderedLocusNames=b0677, z0824, ECs0707;  

 Linear View (Whole Mode)
View Location
   690.0  –  715.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP nagA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Dihydropyrimidinase From Dictyostelium Discoideum  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST N-acetylglucosamine-6-phosphate deacetylase (GlcNAc 6-P deacetylase)  
         PSI-BLAST Urease alpha subunit
    nr     (database updated : 2007.02.20 )
         BLAST N-acetylglucosamine-6-phosphate deacetylase [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG1820G 
    EcoCyc nagA 

MMBR References
Mol Gen Genet. 1980;179(1):49-54.
Characterisation of mutants of Escherichia coli K12, selected by resistance to streptozotocin.
Lengeler J. ( 6450313 )
Biochem Cell Biol. 1990;68(1):123-37.
Cloning and characterization of the N-acetylglucosamine operon of Escherichia coli.
Peri KG, Goldie H, Waygood EB. ( 2190615 )
Mol Gen Genet. 1987;209(3):618-20.
Organisation of the Escherichia coli chromosome between genes glnS and glnU, V.
Plumbridge J. ( 2828887 )
Mol Microbiol. 1989;3(4):505-15.
Sequence of the nagBACD operon in Escherichia coli K12 and pattern of transcription within the nag regulon.
Plumbridge JA. ( 2668691 )
J Bacteriol. 1990;172(5):2728-35.
Induction of the nag regulon of Escherichia coli by N-acetylglucosamine and glucosamine: role of the cyclic AMP-catabolite activator protein complex in expression of the regulon.
Plumbridge JA. ( 2158978 )
Mol Gen Genet. 1989;219(1-2):97-105.
Analysis of the nag regulon from Escherichia coli K12 and Klebsiella pneumoniae and of its regulation.
Vogler AP, Lengeler JW. ( 2693951 )
Biochem J. 1970;118(1):81-7.
An examination of the inhibitory effects of N-iodoacetylglucosamine on Escherichia coli and isolation of resistant mutants.
White RJ, Kent PW. ( 4919471 )

Sequences
Amino acid
FASTA format
0001 MYALTQGRIF TGHEFLDDHA VVIADGLIKS VCPVAELPPE IEQRSLNGAI LSPGFIDVQL NGCGGVQFND 
0071 TAEAVSVETL EIMQKANEKS GCTNYLPTLI TTSDELMKQG VRVMREYLAK HPNQALGLHL EGPWLNLVKK 
0141 GTHNPNFVRK PDAALVDFLC ENADVITKVT LAPEMVPAEV ISKLANAGIV VSAGHSNATL KEAKAGFRAG 
0211 ITFATHLYNA MPYITGREPG LAGAILDEAD IYCGIIADGL HVDYANIRNA KRLKGDKLCL VTDATAPAGA 
0281 NIEQFIFAGK TIYYRNGLCV DENGTLSGSS LTMIEGVRNL VEHCGIALDE VLRMATLYPA RAIGVEKRLG 
0351 TLAAGKVANL TAFTPDFKIT KTIVNGNEVV TQ

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             atttcaatga attagaagca gaaaatatca 
-070 aaggtctgta attgttatcc ctgccctggc tccttgctca gggcaatatt ttttaaaatc gggggtcaga 
0001 atgtatgcat taacccaggg ccggatcttt accggccacg aatttcttga tgaccacgcg gttgttatcg 
0071 ctgatggcct gattaaaagc gtctgtccgg tagcggaact gccgccagag atcgaacaac gttcactgaa 
0141 cggggccatt ctctcccccg gttttatcga tgtgcagtta aacggctgcg gcggcgtaca gtttaacgac 
0211 accgctgaag cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa tgagaaatca ggctgtacta 
0281 actatctgcc gacgcttatc accaccagcg atgagctgat gaaacagggc gtgcgcgtta tgcgcgagta 
0351 cctggcaaaa catccgaatc aggcgttagg tctgcatctg gaaggtccgt ggctgaatct ggtaaaaaaa 
0421 ggcacccata atccgaattt tgtgcgtaag cctgatgccg cgctggtcga tttcctgtgt gaaaacgccg 
0491 acgtcattac caaagtgacc ctggcaccgg aaatggttcc tgcggaagtc atcagcaaac tggcaaatgc 
0561 cgggattgtg gtttctgccg gtcactccaa cgcgacgttg aaagaagcaa aagccggttt ccgcgcgggg 
0631 attacctttg ccacccatct gtacaacgcg atgccgtata ttaccggtcg tgaacctggc ctggcgggcg 
0701 cgatcctcga cgaagctgac atttattgcg gtattattgc tgatggcctg catgttgatt acgccaacat 
0771 tcgcaacgct aaacgtctga aaggcgacaa actgtgtctg gttactgacg ccaccgcgcc agcaggtgcc 
0841 aacattgaac agttcatttt tgcgggtaaa acaatatact accgtaacgg actttgtgtg gatgagaacg 
0911 gtacgttaag cggttcatcc ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt gcggtatcgc 
0981 actggatgaa gtgctacgta tggcgacgct ctatccggcg cgtgcgattg gcgttgagaa acgtctcggc 
1051 acactcgccg caggtaaagt agccaacctg actgcattca cacctgattt taaaatcacc aagaccatcg 
1121 ttaacggtaa cgaggtcgta actcaataag agaaagtatg acaccaggcg gacaagctca gataggtaat 
1191 gttgatctcg taaaacagct taacagcgcg gcggtttatc gcctgattga ccagtacgg