MG1655
W3110
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Gene Report : araB
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion DE(araA-leu)7697  ,  OCR29 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  araB
Related strains
     Strains Search Search strains by gene name:  araB   Search strains by all related name:  araB b0063 ECK0064 JW0062

General information  (Go to Linear View:)
 Gene Name araB  
 Alternative name b0063,ECK0064,JW0062  
 Location, Length 68,348 - 70,048 (  -  ) ;   1.47 min ; 1701 (bp) ,   566 (aa) Go to Linear View
 Product L-ribulokinase  
 Operon Name araBAD  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function enzyme; Degradation of small molecules: Carbon compounds  
 Gene Ontology GO:0005515; protein binding ( araB )
GO:0005975; carbohydrate metabolic process ( araB )
GO:0008741; ribulokinase activity ( araB )
GO:0016301; kinase activity ( araB )
GO:0016740; transferase activity ( araB )
GO:0019568; arabinose catabolic process ( araB )
GO:0019572; L-arabinose catabolic process ( araB )
 PID 1786249  
 EC number
  (KEGG Pathway)
 
2.7.1.16  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene araB ( EG10053 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) ARAB_ECOLI ( P08204 ; P78041 )
    -  Protein name Ribulokinase  
    -  Synonyms EC 2.7.1.16  
    -  Gene name Name=araB; OrderedLocusNames=b0063;  

 Linear View (Whole Mode)
View Location
   60.0  –  85.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP araB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain O, Enterococcus Casseliflavus Glycerol Kinase  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Ribulokinase  
         PSI-BLAST Glycerol kinase 2
    nr     (database updated : 2007.02.20 )
         BLAST ribulokinase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION     

Other Cross-Reference
    COG COG1069C 
    EcoCyc araB 

MMBR References
J Mol Biol. 1984;180(1):201-4.
Deletion analysis of the Escherichia coli ara PC and PBAD promoters.
Dunn TM, Schleif R. ( 6392566 )
J Bacteriol. 1970;102(2):593-5.
Selection of araB and araC mutants of Escherichia coli B-r by resistance to ribitol.
Katz L. ( 4911550 )
Gene. 1986;47(2-3):231-44.
The organization of the araBAD operon of Escherichia coli.
Lee N, Gielow W, Martin R, Hamilton E, Fowler A. ( 3549454 )
Proc Natl Acad Sci U S A. 1981;78:752-756
Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and Pbad, in the L-arabinose regulatory region of Escherichia coli.
Lee, N., W. Gielow, and R. G. Wallace.
Gene. 1982;17(2):167-77.
The use of synthetic oligodeoxyribonucleotides to produce specific deletions in the araBAD promoter of Escherichia coli B/r.
Miyada CG, Soberon X, Itakura K, Wilcox G. ( 7044899 )
Proc Natl Acad Sci U S A. 1980;77:3346-3350
The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation.
Ogden, S., D. Haggerty, C. M. Stoner, D. Kolodrubetz, and R. Schleif.
J Biol Chem. 1978;253:6931-6933
Nucleotide sequence of the L-arabinose regulatory region of Escherichia coli K12.
Smith, B. R., and R. Schleif.

Sequences
Amino acid
FASTA format
0001 MAIAIGLDFG SDSVRALAVD CATGEEIATS VEWYPRWQKG QFCDAPNNQF RHHPRDYIES MEAALKTVLA 
0071 ELSVEQRAAV VGIGVDSTGS TPAPIDADGN VLALRPEFAE NPNAMFVLWK DHTAVEEAEE ITRLCHAPGN 
0141 VDYSRYIGGI YSSEWFWAKI LHVTRQDSAV AQSAASWIEL CDWVPALLSG TTRPQDIRRG RCSAGHKSLW 
0211 HESWGGLPPA SFFDELDPIL NRHLPSPLFT DTWTADIPVG TLCPEWAQRL GLPESVVISG GAFDCHMGAV 
0281 GAGAQPNALV KVIGTSTCDI LIADKQSVGE RAVKGICGQV DGSVVPGFIG LEAGQSAFGD IYAWFGRVLG 
0351 WPLEQLAAQH PELKTQINAS QKQLLPALTE AWAKNPSLDH LPVVLDWFNG RRTPNANQRL KGVITDLNLA 
0421 TDAPLLFGGL IAATAFGARA IMECFTDQGI AVNNVMALGG IARKNQVIMQ ACCDVLNRPL QIVASDQCCA 
0491 LGAAIFAAVA AKVHADIPSA QQKMASAVEK TLQPCSEQAQ RFEQLYRRYQ QWAMSAEQHY LPTSAPAQAA 
0561 QAVATL

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             atagcatttt tatccataag attagcggat 
-070 cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg 
0001 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggac tgcgctaccg 
0071 gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg caattttgtg atgccccgaa 
0141 taaccagttc cgtcatcatc cgcgtgacta cattgagtca atggaagcgg cactgaaaac cgtgcttgca 
0211 gagcttagcg tcgaacagcg cgcagctgtg gtcgggattg gcgttgacag taccggctcg acgcccgcac 
0281 cgattgatgc cgacggaaac gtgctggcgc tgcgcccgga gtttgccgaa aacccgaacg cgatgttcgt 
0351 attgtggaaa gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac 
0421 gttgactact cccgctacat tggtggtatt tattccagcg aatggttctg ggcaaaaatc ctgcatgtga 
0491 ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg tgcgactggg tgccagctct 
0561 gctttccggt accacccgcc cgcaggatat tcgtcgcgga cgttgcagcg ccgggcataa atctctgtgg 
0631 cacgaaagct ggggcggcct gccgccagcc agtttctttg atgagctgga cccgatcctc aatcgccatt 
0701 tgccttcccc gctgttcact gacacttgga ctgccgatat tccggtgggc accttatgcc cggaatgggc 
0771 gcagcgtctc ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt 
0841 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt ctgattgccg 
0911 acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt gatggcagcg tggtgcctgg 
0981 atttatcggt ctggaagcag gccaatcggc gtttggtgat atctacgcct ggtttggtcg cgtactcggc 
1051 tggccgctgg aacagcttgc cgcccagcat ccggaactga aaacgcaaat caacgccagc cagaaacaac 
1121 tgcttccggc gctgaccgaa gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg 
1191 gtttaacggc cgccgcacac cgaacgctaa ccaacgcctg aaaggggtga ttaccgatct taacctcgct 
1261 accgacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca atcatggagt 
1331 gctttaccga tcaggggatc gccgttaata acgtgatggc actgggcggc atcgcgcgga aaaaccaggt 
1401 cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg caaattgttg cctctgacca gtgctgtgcg 
1471 ctcggtgcgg cgatttttgc tgccgtcgcc gcgaaagtgc acgcagacat cccatcagct cagcaaaaaa 
1541 tggccagtgc ggtagagaaa accctgcaac cgtgcagcga gcaggcacaa cgctttgaac agctttatcg 
1611 ccgctatcag caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc 
1681 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg gtttgtcatt 
1751 ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca t