MG1655
W3110
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Gene Report : holA
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Annu Rev Biochem. 1995;64:171-200.
DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.
Kelman Z, O'Donnell M. ( 7574479 )
     Deletion OCR46-11 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  holA
Related strains
     Strains Search Search strains by gene name:  holA   Search strains by all related name:  holA b0640 ECK0633 JW0635

General information  (Go to Linear View:)
 Gene Name holA  
 Alternative name b0640,ECK0633,JW0635  
 Location, Length 669,797 - 670,828 (  -  ) ;   14.44 min ; 1032 (bp) ,   343 (aa) Go to Linear View
 Product DNA polymerase III, delta subunit  
 Operon Name leuS-lptE-holA-nadD-cobC  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication  
 Function enzyme; DNA - replication, repair,restriction/modification  
 Gene Ontology GO:0003677; DNA binding ( holA )
GO:0003887; DNA-directed DNA polymerase activity ( holA )
GO:0003891; delta DNA polymerase activity ( holA )
GO:0005515; protein binding ( holA )
GO:0005634; nucleus ( holA )
GO:0006260; DNA replication ( holA )
GO:0009360; DNA polymerase III complex ( holA )
GO:0016740; transferase activity ( holA )
GO:0016779; nucleotidyltransferase activity ( holA )
GO:0043625; delta DNA polymerase complex ( holA )
 PID 1786859  
 EC number
  (KEGG Pathway)
 
2.7.7.7  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene holA ( EG11412 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) HOLA_ECOLI ( P28630 )
    -  Protein name DNA polymerase III, delta subunit  
    -  Synonyms EC 2.7.7.7  
    -  Gene name Name=holA; OrderedLocusNames=b0640;  

 Linear View (Whole Mode)
View Location
   660.0  –  685.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP holA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain D, Crystal Structure Of The Processivity Clamp Loader Gamma Complex Of E. Coli Dna Polymerase Iii  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST DNA polymerase III subunit delta  
         PSI-BLAST Holliday junction ATP-dependent DNA helicase ruvB
    nr     (database updated : 2007.02.20 )
         BLAST DNA polymerase III subunit delta [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG1466L 
    EcoCyc holA 

MMBR References
J Biol Chem. 1993;268:11758-11765
DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'.
Dong, Z., R. Onrust, M. Skangalis, and M. E. O'Donnell.
Annu Rev Biochem. 1995;64:171-200.
DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.
Kelman Z, O'Donnell M. ( 7574479 )
J Biol Chem. 1993;268:11766-11772
DNA polymerase III accessory proteins. II. Characterization of delta and delta'.
Onrust, R., and M. O'Donnell.
J Bacteriol. 1992;174:7013-7025
Molecular cloning, sequencing, and overexpression of the structural gene encoding the subunit of Escherichia coli DNA polymerase III holoenzyme.
Carter, J. R., M. A. Franden, R. Aebersold, and C. S. McHenry.

Sequences
Amino acid
FASTA format
0001 MIRLYPEQLR AQLNEGLRAA YLLLGNDPLL LQESQDAVRQ VAAAQGFEEH HTFSIDPNTD WNAIFSLCQA 
0071 MSLFASRQTL LLLLPENGPN AAINEQLLTL TGLLHDDLLL IVRGNKLSKA QENAAWFTAL ANRSVQVTCQ 
0141 TPEQAQLPRW VAARAKQLNL ELDDAANQVL CYCYEGNLLA LAQALERLSL LWPDGKLTLP RVEQAVNDAA 
0211 HFTPFHWVDA LLMGKSKRAL HILQQLRLEG SEPVILLRTL QRELLLLVNL KRQSAHTPLR ALFDKHRVWQ 
0281 NRRGMMGEAL NRLSQTQLRQ AVQLLTRTEL TLKQDYGQSV WAELEGLSLL LCHKPLADVF IDG

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gcatccgtgc tgcggatatt cgttccgacg 
-070 aagaacagac gtcgaccaca acggatactc cggcaacgcc tgcacgcgtc tccaccacgc tgggtaactg 
0001 atgattcggt tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg tatcttttac 
0071 ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt 
0141 cgaagaacac cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct 
0211 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat gcggcgatca 
0281 atgagcaact tctcacactc accggacttc tgcatgacga cctgctgttg atcgtccgcg gtaataaatt 
0351 aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt gcgaatcgca gcgtgcaggt gacctgtcag 
0421 acaccggagc aggctcagct tccccgctgg gttgctgcgc gcgcaaaaca gctcaactta gaactggatg 
0491 acgcggcaaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg 
0561 tttatcgctg ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg 
0631 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcgcattg catattcttc 
0701 agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta caacgtgaac tgttgttact 
0771 ggttaacctg aaacgccagt ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag 
0841 aaccgccggg gcatgatggg cgaggcgtta aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac 
0911 tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt 
0981 atctcttctg ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc 
1051 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct ggcgaatttg 
1121 attggtctga cg